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Image Search Results
Journal: EJNMMI Research
Article Title: Evaluation of retinol binding protein 4 and carbamoylated haemoglobin as potential renal toxicity biomarkers in adult mice treated with 177 Lu-octreotate
doi: 10.1186/s13550-014-0059-x
Figure Lengend Snippet: Mean absolute urinary RBP4 and creatinine concentrations and RBP4/creatinine concentration ratios
Article Snippet: RBP4 in urine samples (50 μL diluted to 100 μL) was analysed using the
Techniques: Concentration Assay
Journal: EJNMMI Research
Article Title: Evaluation of retinol binding protein 4 and carbamoylated haemoglobin as potential renal toxicity biomarkers in adult mice treated with 177 Lu-octreotate
doi: 10.1186/s13550-014-0059-x
Figure Lengend Snippet: Analysis of urinary RBP4. Mean differences between the log 10 -transformed mean values (from three consecutive days) and the log 10 -transformed baseline mean values (from three consecutive days) for (A) creatinine and (B) RBP4 and (C) RBP4/creatinine, in non-treated mice (blue circle, n = 4 to 6), and in mice treated with 60 MBq (red square, n = 5 to 6) or 120 MBq 177 Lu-octreotate (green diamond, n = 5 to 6) vs. time after study start. Log 10 -transformation was done since the values are skewed (see ‘ ’). All values are shown as mean ± SEM. Upward arrows indicate that the mean RBP4 value is higher and downward arrows that it is lower than presented (see text). The heavy asterisk indicates data statistically significant different from baseline ( p <0.05).
Article Snippet: RBP4 in urine samples (50 μL diluted to 100 μL) was analysed using the
Techniques: Transformation Assay
Journal: The Journal of Biological Chemistry
Article Title: The retinol-binding protein receptor STRA6 regulates diurnal insulin responses
doi: 10.1074/jbc.M117.782334
Figure Lengend Snippet: Serum RBP4 and TTR levels display circadian rhythmicity. A, serum RBP4 levels over a 24-h period in WT and STRA6-null mice. * indicates significant difference of at least p < 0.05 between WT at ZT10 and ZT14. B, serum TTR levels over a 24-h period in WT and STRA6-null mice. * indicates significant difference in WT between ZT2 and ZT10. ** indicates significant difference in KO between ZT2 and ZT10. C, liver Rbp4 mRNA expression over a 24-h period in WT and STRA6-null mice. D, WAT Rbp4 mRNA expression over a 24-h period in WT and STRA6-null mice. Data shown are double-plotted data. Data are represented as mean ± S.E.
Article Snippet: Serum RBP4 was measured using a
Techniques: Expressing
Journal: International Journal of Proteomics
Article Title: The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
doi: 10.1155/2011/628787
Figure Lengend Snippet: (a) Magnified views of 2-D gel images and western blotting analysis of ApoA-IV, GC, plasma retinol binding protein 4 RBP4, and CLEC3B in serum samples. Coomassie blue-stained 2-D gel images from pooled control and pancreatic cancer displaying the protein spots for ApoA-IV, GC, RBP4, and CLEC3B are shown in left panels (a1), (a2), (a3) and (a4). Western blotting of these four proteins are shown in the right panel (b1), (b2), (b3) and (b4). (b) Quantitation of differentially expressed serum proteins in pancreatic cancer and healthy volunteers by Western blot analysis. Intensities of each band were calculated by TotalLab TL 120 software. Closed circles indicate healthy volunteers and closed squares indicate patients with pancreatic cancer. Significance of the differences were calculated by using Wilcoxon Mann-Whitney test. Panel A: ApoA-IV levels of serum were significantly lower in the depleted sera of pancreatic cancer when compared with the depleted sera of healthy volunteers ( P < 0.0001). Panel (B, C and D) are for proteins GC, RBP4, and CLEC3B and their serum levels were likewise lower in the pancreatic cancer patients. Their P values are lower than 0.0001, 0.001, and 0.009, respectively.
Article Snippet: The following antibodies commercially available were used as primary antibodies; mouse anti-human ApoA-IV antibody (BML Inc., Tokyo, Japan), mouse anti-human GC antibody (LifeSpan, Inc., UK),
Techniques: Western Blot, Binding Assay, Staining, Quantitation Assay, Software, MANN-WHITNEY
Journal: International Journal of Proteomics
Article Title: The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
doi: 10.1155/2011/628787
Figure Lengend Snippet: Quantitation of differentially expressed proteins in pancreatic cancer by ELISA. ELISA was performed using human ApoA-IV ELISA kit vitamin D binding protein ELISA kit and RBP4 ELISA kit, in serum samples obtained from 15 patients with pancreatic cancers and 15 control subjects. Analysis was performed by using Wilcoxon Mann-Whitney test. Closed circles indicate control and closed squares indicate cancer. (a): ApoA-IV levels of serum of patient with pancreatic cancer group (107.76 ± 25.8 AU) were lower than those in healthy group (185.27 ± 16.0 AU, P = 0.01); (b): GC levels were significantly lower in the patient group with pancreatic cancer (25.35 ± 9.8 UA) when compared with healthy group (34.40 ± 10.2 AU, P = 0.03); (c): RBP4 levels were lower in the pancreatic cancer group (42.99 ± 1.5 AU) than healthy group (50.7 ± 1.00 AU, P < 0.001).
Article Snippet: The following antibodies commercially available were used as primary antibodies; mouse anti-human ApoA-IV antibody (BML Inc., Tokyo, Japan), mouse anti-human GC antibody (LifeSpan, Inc., UK),
Techniques: Quantitation Assay, Enzyme-linked Immunosorbent Assay, Binding Assay, MANN-WHITNEY
Journal: International Journal of Proteomics
Article Title: The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
doi: 10.1155/2011/628787
Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for CA19-9, CEA, ApoA-IV, GC, RBP4 Their AUCs are described in the text.
Article Snippet: The following antibodies commercially available were used as primary antibodies; mouse anti-human ApoA-IV antibody (BML Inc., Tokyo, Japan), mouse anti-human GC antibody (LifeSpan, Inc., UK),
Techniques:
Journal: International Journal of Proteomics
Article Title: The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
doi: 10.1155/2011/628787
Figure Lengend Snippet: (a) Serum tumor marker levels in patients with pancreatic cancer.
Article Snippet: The following antibodies commercially available were used as primary antibodies; mouse anti-human ApoA-IV antibody (BML Inc., Tokyo, Japan), mouse anti-human GC antibody (LifeSpan, Inc., UK),
Techniques: Marker
Journal: International Journal of Proteomics
Article Title: The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
doi: 10.1155/2011/628787
Figure Lengend Snippet: (b) Serum tumor marker levels in healthy controls.
Article Snippet: The following antibodies commercially available were used as primary antibodies; mouse anti-human ApoA-IV antibody (BML Inc., Tokyo, Japan), mouse anti-human GC antibody (LifeSpan, Inc., UK),
Techniques: Marker