Journal: The Journal of Neuroscience

Article Title: A Novel Role for Protein Tyrosine Phosphatase SHP1 in Controlling Glial Activation in the Normal and Injured Nervous System

doi: 10.1523/JNEUROSCI.21-03-00865.2001

Figure Lengend Snippet: Astrocytic and microglial reaction in the intact brain during adulthood, in the intact (Con) and axotomized (Ax) facial nucleus in normal, wild-type littermates (WT) and SHP1 mutant, moth-eaten viable mice (MEV). A, GFAP-immunoreactivity in the brainstem of WT and MEV mice at postnatal days 4 (P4) and 14 (P14). Note that GFAP-IR is increased in MEV mice compared with the same brain regions of WT littermates. B, GFAP-IR in the brainstem of WT and MEV mice 3 d after facial nerve transection. Note that GFAP-IR is increased on the control, unoperated side in MEV mice (Con-MEV) (as well as in the whole brain) compared with the same region of normal littermates (Con-WT). C, The morphology of microglia, detected by αMβ2-integrin immunoreactivity 2 and 3 d after facial nerve axotomy. Note that after facial nerve axotomy, the microglial cells in MEV mice are characterized by rounded, ameboid morphology with delayed and decreased ramification. D, Combination of [3H]-thymidine autoradiography (white points) with either GFAP-immunoreactivity (green) or αMβ2-integrin-IR (red) 3 d after facial nerve axotomy. Note that the proliferating cells in MEV mice are exclusively αMβ2-integrin-positive microglia. Scale bars, 100 μm.

Article Snippet: Mouse brain sections (20 μm) from normal littermates and mutant me v /me v mice were incubated with either rat monoclonal antibodies against α M β 2 -integrin (1:6000, Serotec) or rat monoclonal antibodies against GFAP (Zymed).

Techniques: Mutagenesis, Control, Autoradiography

Journal: The Journal of Neuroscience

Article Title: A Novel Role for Protein Tyrosine Phosphatase SHP1 in Controlling Glial Activation in the Normal and Injured Nervous System

doi: 10.1523/JNEUROSCI.21-03-00865.2001

Figure Lengend Snippet: Quantitative effects of reduced SHP1 activity on astrocytic (A) and microglial reaction (B) in the intact and injured brain as well as on axonal outgrowth (C). A, Quantification of GFAP-IR (pixel number) in the intact brain (random brainstem area, nonaxotomized facial nucleus) and 3 d after facial nerve axotomy in wild-type (WT) littermates and MEV mice. Note that only in the uninjured brain regions there is a statistically significant increase for GFAP-IR in MEV mice compared with WT mice (6 sections per each animal; n = 4 animals). B1, Quantification of αMβ2-integrin-positive microglia in intact brain regions (random brainstem area and nonaxotomized facial nucleus). Note that the number of resting microglia is similar in WT and MEV mice. B2, Reduction in the number of proliferating ([3H]-thymidine-labeled) αMβ2-positive microglia in MEV mice 2 d after direct cortical lesion. B3, Reduction in the number of αMβ2-positive microglia and the proliferation rate ([3H]-thymidine-labeled cells) in MEV mice 2 and 3 d after facial nerve axotomy compared with wild-type littermates. The microglial reaction was always quantified in six sections per animal (n = 4). C, Four days after crush near the foramen stylomastoideum, the facial nerve was cut longitudinally and stained for galanin or CGRP, which accumulate in the terminals of the elongating neurites. The average distance between the most distal-labeled growth cone and the crush side was determined for each axonal marker in five tissue sections per animal (n = 6). Both the galanin- and CGRP-positive axonal populations show the same regeneration distance of ∼6 mm at day 4 in the WT littermates and MEV mice. All statistical analyses were performed using a paired, two-tailed Student's t test. Statistically significant changes are indicated byasterisks (*p < 0.05, ***p < 0.001).

Article Snippet: Mouse brain sections (20 μm) from normal littermates and mutant me v /me v mice were incubated with either rat monoclonal antibodies against α M β 2 -integrin (1:6000, Serotec) or rat monoclonal antibodies against GFAP (Zymed).

Techniques: Activity Assay, Labeling, Staining, Marker, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: Genetic Background and Kinetics Define Wound Bed Extracellular Vesicles in a Mouse Model of Cutaneous Injury

doi: 10.3390/ijms22073551

Figure Lengend Snippet: Antibodies used for analysis of EV surface markers using vesicle flow cytometry.

Article Snippet: CD18 , M18/2 , APC , 130-104-019 , Miltenyi Biotec.

Techniques: Cytometry