Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17B and IL-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis.

doi: 10.4049/jimmunol.179.10.7128

Figure Lengend Snippet: FIGURE 1. The expression of IL-17 family members and IL-17R genes in the arthritic paws of CIA mice. A, The expressions of IL-17 family genes and IL-17R genes were examined in the arthritic paws of CIA mice (f; n 3) and in control mice (; n 3) by quantitative PCR. B, The expressions of inflammatory cy- tokines. C, The expressions of IL-17 family members in the sorted cell populations of the arthritic paws of CIA mice. The data are representative of three independent experiments.

Article Snippet: Mice exhibited the first clinical signs of arthritis (arthritis score between 1 and 2) and were injected i.p. with 100 g of polyclonal anti-mouse IL-17B Abs (R&D Systems).

Techniques: Expressing, Control, Real-time Polymerase Chain Reaction

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17B and IL-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis.

doi: 10.4049/jimmunol.179.10.7128

Figure Lengend Snippet: FIGURE 2. The proinflammatory effects of IL-17 family members on mouse fibroblasts and macrophages. A, Relative expression of the cytokine genes in 3T3 cell. The mouse fibroblast cell line 3T3 was cultured with each of mIL-17A, mIL-17B, mIL-17C, or mIL-17F for 24 h, and the expressions of inflammatory cytokines were measured by quantitative PCR. B, Relative expression of the cytokine genes in mouse thioglycolate-elicited PECs. PECs were cultured with each of mIL-17A, mIL-17B, mIL-17C, or mIL-17F for 24 h, and the expressions of inflammatory cytokines were measured by quantitative PCR. C, The secreted IL-6 and TNF- levels in the supernatants of 3T3 and PECs were measured by ELISA. Error bars indicate SD. The data are representative of three independent experiments. Significance of differences between control (medium) and each IL-17 family was determined; , p 0.05.

Article Snippet: Mice exhibited the first clinical signs of arthritis (arthritis score between 1 and 2) and were injected i.p. with 100 g of polyclonal anti-mouse IL-17B Abs (R&D Systems).

Techniques: Expressing, Cell Culture, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17B and IL-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis.

doi: 10.4049/jimmunol.179.10.7128

Figure Lengend Snippet: FIGURE 3. The effects of transfer of IL-17 family-transduced CD4 T cells on CIA. A, Intracellular IL-17 family expressions in Ba/F3 cells ret- rovirally transduced with each IL-17 family member. GFP-gated IL-17 family-transduced (mIL-17A, mIL- 17B, mIL-17C, or mIL-17F) Ba/F3 cells were analyzed for IL-17A, IL- 17B, IL-17C, or IL-17F expression compared with GFP-gated empty vector (pMIG)-transduced Ba/F3 cells. B, Representative FACS analy- sis of IL-17 family-transduced CD4

Article Snippet: Mice exhibited the first clinical signs of arthritis (arthritis score between 1 and 2) and were injected i.p. with 100 g of polyclonal anti-mouse IL-17B Abs (R&D Systems).

Techniques: Transduction, Expressing, Plasmid Preparation

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17B and IL-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis.

doi: 10.4049/jimmunol.179.10.7128

Figure Lengend Snippet: FIGURE 4. Generation of IL-17 family chimeric mice by BM trans- plantation of gene-transduced BM cells. Each of IL-17 family genes was transduced to BM cells with retrovirus vector and transferred to lethally irradiated mice. A, The intracellular expression of IL-17A protein in the spleen of IL-17A BM chimeric mice 8 wk after BM transplantation. The percentage of GFP cells expressing IL-17A is indicated. The data are representative of three independent experiments. B, The concentration of IL-17A protein in the serum of IL-17A BM chimeric mice (n 6) and control mice (pMIG BM chimeric mice) (n 6). The levels of IL-17A were measured by ELISA.

Article Snippet: Mice exhibited the first clinical signs of arthritis (arthritis score between 1 and 2) and were injected i.p. with 100 g of polyclonal anti-mouse IL-17B Abs (R&D Systems).

Techniques: Plasmid Preparation, Irradiation, Expressing, Transplantation Assay, Concentration Assay, Control, Enzyme-linked Immunosorbent Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17B and IL-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis.

doi: 10.4049/jimmunol.179.10.7128

Figure Lengend Snippet: FIGURE 5. Incidence of CIA and arthritis scores in IL-17 family BM chimeric mice. Incidence of CIA and arthritis scores in IL-17A and IL-17F BM chimeric mice (A and B), and in IL-17B and IL-17C BM chimeric mice (C and D). Mice were immu- nized with BCII 8 wk after the BM transplantation. Values are the mean of experiments for IL-17A and IL-17F BM chimeric mice (n 20 per group) and experiments for IL-17B and IL-17C BM chimeric mice (n 30 per group). Significance of differences between control (pMIG) and each IL-17 family BM chimeric mice was determined; , p 0.005; , p 0.05. E, The mRNA expression of inflammatory cytokines in the spleen of BM chi- meric mice of IL-17B and IL-17C, which were immunized with BCII (f; n 15 per group) or nonimmunized controls (; n 6 per group). Sig- nificance of differences between con- trol (pMIG) and each IL-17 family BM chimeric mice was determined; , p 0.005; , p 0.05. F, The secreted TNF- and IL-6 levels in the serum of IL-17 family BM chimeric mice that were immunized with BCII (f; n 15) or nonimmunized con- trols (; n 6). Significance of dif- ferences between control (pMIG) and each IL-17 family BM chimeric mice was determined; , p 0.005; , p 0.05.

Article Snippet: Mice exhibited the first clinical signs of arthritis (arthritis score between 1 and 2) and were injected i.p. with 100 g of polyclonal anti-mouse IL-17B Abs (R&D Systems).

Techniques: Transplantation Assay, Control, Expressing

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17B and IL-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis.

doi: 10.4049/jimmunol.179.10.7128

Figure Lengend Snippet: FIGURE 6. Effect of anti-IL-17B Ab treatment in CIA mice. A, CIA mice received i.p. injection of anti-mouse IL-17B Abs after the first clinical signs of arthritis (arthritis score between 1 and 2). As a control, PBS was injected. The arthritis score was shown. B, Histological score of the inflammatory joints of CIA mice treated with anti-IL-17B Abs was evaluated at 10 days after the onset of arthritis. Cellular infiltration and pannus invasion were graded in all four paws of the mice. Values are the mean of arthritis scores for anti-IL-17B Ab-treated mice and control mice (n 5 per group). Significance of differences between control and anti-IL-17B Ab-treated mice was shown.

Article Snippet: Mice exhibited the first clinical signs of arthritis (arthritis score between 1 and 2) and were injected i.p. with 100 g of polyclonal anti-mouse IL-17B Abs (R&D Systems).

Techniques: Injection, Control

Journal: bioRxiv

Article Title: Endothelial CXCL5 negatively regulates myelination and repair after white matter stroke

doi: 10.1101/664953

Figure Lengend Snippet: Schematic representation of IL-17/CXCL5 signaling in chronically injured cerebral endothelia (A). Human brain microvascular endothelial cells were stimulated with IL-17 ligands A-E (250 ng/mL) and CXCL5 levels measured in conditioned media 48 hrs after stimulation (* p =0.0372 by Kruskal-Wallis H test; **post-hoc comparison for IL-17B vs. no ligand, adjusted p =0.0178) (B). Weight adjusted-ELISA values (pg/mL) for murine CXCL5 in retro-orbital blood samples from CFD (black) and HFD (red) animals ( n =4/grp, p =0.0355) (C). Immunofluorescence labeling for IL-17Rb (green, D) and CXCL5 (green, E) is absent in white matter vasculature of Tie2-Cre;tdTomato mice on CFD (left panels) and abundant in white matter vasculature of Tie2-Cre;tdTomato mice on HFD (right panels). Single channel labeling for IL17Rb (lower panels, D) and CXCL5 (lower panels, E) show heterogenous endothelial expression. CXCL5 is detected in human frontal white matter endothelia from aged subjects. Bar represents percentage of cases (8/10) with any CXCL5 staining with points indicating the individual percentage of CXCL5-positive vessel segments in individuals with (blue) and without (red) vascular dementia (F). Scale bars = 50 μm in F, 20 μm in D, 10 μm in E.

Article Snippet: Anti-mIL-17B function blocking antibody (R&D, AF1709) was diluted with 0.9% saline to a concentration of 1mg/ml.

Techniques: Enzyme-linked Immunosorbent Assay, Immunofluorescence, Labeling, Expressing, Staining

Journal: bioRxiv

Article Title: Endothelial CXCL5 negatively regulates myelination and repair after white matter stroke

doi: 10.1101/664953

Figure Lengend Snippet: Association of PDGFRα+ OPCs (green) with the vasculature (blue) in CFD (upper panel) and CXCL5-positive (red) HFD animals (lower panel) (A). Phalloidin-positive cellular area in O4+ OPCs grown in vitro exposed to vehicle (upper panel) or recombinant CXCL5 (lower panel) for 48 h ( p <0.0001, F=9.82 by one-way ANOVA). Experimental approach for CXCL5 transgenic-viral gain of function (upper panel, C). PDGFRα+ OPC (green) labeling in GFP-transduced Tie2-Cre;tdTomato mice (red, left panel) and CXCL5-GFP-transduced Tie2-Cre;tdTomato mice (right panel). Representative masked cellular profiles of PDGFRα+ cell area (lower panels). Schematic of anti-IL-17B antibody treatment (upper panel, D). PDGFRα+ OPC (green) labeling in control IgG-treated Tie2-Cre:tdT mice (left panel) and anti-IL-17B IgG-treated Tie2-Cre:tdT mice (right panel). Representative masked cellular profiles of PDGFRα+ cell area (lower panels). Proportion of OPCs per unit distance from vessel (0-35 μm) in each condition (total measured cell number per condition in parentheses) (E). Average distance of OPCs to vessel (*** p =0.0005, F=6.06 by one-way ANOVA; **adjusted p =0.0039; *adjusted p =0.0168) (F). Average in vivo PDGFRα+ OPC cell area (** p =0.0068, F=7.38 by one-way ANOVA; **adjusted p =0.002) (G). Graph of co-localized CXCL5+/GLUT-1+ voxels in anti-IL-17B IgG-treated animals ( n= 4/grp; *p =0.018). (H). Scale bars = 10 μm.

Article Snippet: Anti-mIL-17B function blocking antibody (R&D, AF1709) was diluted with 0.9% saline to a concentration of 1mg/ml.

Techniques: In Vitro, Recombinant, Transgenic Assay, Labeling, In Vivo

Journal: bioRxiv

Article Title: Endothelial CXCL5 negatively regulates myelination and repair after white matter stroke

doi: 10.1101/664953

Figure Lengend Snippet: Subjects presenting for evaluation of acute neurologic deficits were enrolled in the ASPIRE study cohort (A). In all subjects, serum levels of CXCL5 were elevated in those subjects with detectable serum IL-17B ( n =32; median 1043.0 pg/mL) compared to those with undetectable serum IL-17B ( n =99; median 515.3 pg/mL; * p <0.0001; Mann-Whitney). Among those subjects with MRI-confirmed acute microvascular ischemia, serum CXCL5 levels were higher in IL-17B+ subjects ( n =9; 978.2 pg/mL) vs. IL-17B-subjects ( n =24; 539.7 pg/mL) (** p =0.0157; Mann Whitney) (B). Box and violin plot of modified Fazekas score in IL-17B+ vs. IL-17B-subjects (dashed line = median) ( p =0.42; Mann-Whitney U-test) (C).

Article Snippet: Anti-mIL-17B function blocking antibody (R&D, AF1709) was diluted with 0.9% saline to a concentration of 1mg/ml.

Techniques: MANN-WHITNEY, Modification

Journal: Bioengineered

Article Title: MicroRNA-155-5p modulates the progression of acute respiratory distress syndrome by targeting interleukin receptors

doi: 10.1080/21655979.2022.2071020

Figure Lengend Snippet: Identification of IL17RB, IL18R1, and IL22RA2 as potential targets of miR-155-5p. IL17RB, IL18R1, and IL22RA2 were identified as potential targets of miR-155-5p by the databases (a); this was confirmed by the dual-luciferase report assay (b). The levels of IL17RB, IL18R1, and IL22RA2 were measured by qRT-PCR (c). *p < 0.05 and **p < 0.01, ANOVA.

Article Snippet: In brief, LPS reagent was injected into the medium for 24 hours and the relative levels of human IL17RB (EK0785), IL18R1 (EK1260), IL22RA2, IL-1β, IL-6, IL-8, and TNF-α were measured in the treated cells using an ELISA kit (Boster, Wuhan, China) in accordance with the manufacturer’s protocol [ ].

Techniques: Luciferase, Quantitative RT-PCR

Journal: Bioengineered

Article Title: MicroRNA-155-5p modulates the progression of acute respiratory distress syndrome by targeting interleukin receptors

doi: 10.1080/21655979.2022.2071020

Figure Lengend Snippet: Upregulated levels of the inflammatory cytokines in patients with ARDS. The levels of IL17RB, IL18R1, and IL22RA2 in serum samples from patients with ARDS (a). Serum concentrations of IL17RB, IL18R1, and IL22RA2 (b), and IL-1β, IL-6, IL-8, and TNF-α (c) in patients with ARDS were analyzed by ELISA. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control.

Article Snippet: In brief, LPS reagent was injected into the medium for 24 hours and the relative levels of human IL17RB (EK0785), IL18R1 (EK1260), IL22RA2, IL-1β, IL-6, IL-8, and TNF-α were measured in the treated cells using an ELISA kit (Boster, Wuhan, China) in accordance with the manufacturer’s protocol [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Bioengineered

Article Title: MicroRNA-155-5p modulates the progression of acute respiratory distress syndrome by targeting interleukin receptors

doi: 10.1080/21655979.2022.2071020

Figure Lengend Snippet: miR-155-5p inhibited the NF-kB signaling pathway. The expression levels of the inflammatory cytokine receptors (IL17RB, IL18R1, and IL22R2) and NF-kB-related proteins (NF-kB, STAT1, and STAT3) were measured by western blotting analysis with β-actin used as the internal control. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control.

Article Snippet: In brief, LPS reagent was injected into the medium for 24 hours and the relative levels of human IL17RB (EK0785), IL18R1 (EK1260), IL22RA2, IL-1β, IL-6, IL-8, and TNF-α were measured in the treated cells using an ELISA kit (Boster, Wuhan, China) in accordance with the manufacturer’s protocol [ ].

Techniques: Expressing, Western Blot, Control