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  • 99
    Thermo Fisher gene exp il6 mm00446190 m1
    Chronic CS exposure increases BAL fluid cellularity and enhances both lung pro‐inflammatory and oxidative stress mediator gene expression. The lungs of mice exposed to CS were lavaged for the assessment of total cells (a), macrophages (b), neutrophils (c), and lymphocytes (d) ( n = 10). Whole lungs excised from mice were then used to measure mRNA expression by RT‐qPCR of TNFα (e) ( n = 10), <t>IL‐6</t> (f) ( n = 10), and NOX‐2 (g) ( n = 9). Gene expression data are expressed as fold change relative to the sham group. All data are expressed as mean + SEM. * P
    Gene Exp Il6 Mm00446190 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp il6 mm00446190 m1/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp il6 mm00446190 m1 - by Bioz Stars, 2021-05
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    99
    R&D Systems mouse il 6
    Lack of effect of <t>IL‐6</t> on GLP‐1 Secretion in the Perfused Mouse Small Intestine and in GLP‐1 producing GLUTag cells. (A) GLP‐1 secretion (pmol/L) in perfused mouse small intestines ( n = 6). Infusion of IL‐6 (100 ng/mL) (10–20 min), followed by a period in the absence of IL‐6 (21–39 min), and finally, a period in the presence of 10 nmol/L of bombesin (positive control; 40–45 min). (B) Hormone output (secretion) calculated as area under the curve, with (gray) or without (white) IL‐6 (100 ng/mL), was calculated for each mouse and illustrated collectively as bar and whiskers (Tukey distribution). (C) IL‐6 bioactivity in perfusion samples (#1‐#4) was determined by the ability of these perfusates to induce STAT3 phosphorylation (P‐STAT3) as analyzed by SDS‐PAGE and Western blotting. One‐hundred ng/mL of mIL‐6 was used as positive control (pos.) and Krebs‐Ringer bicarbonate buffer as negative control (neg.). Total STAT3 and α ‐tubulin were used as loading controls. (D) GLP‐1 levels in cell media after 2 h incubation with buffer, 100 or 1000 ng/mL IL‐6, or 10 mmol/L glucose ( n = 4). IL‐6 had no effect on GLP‐1 secretion compared to basal levels in either of these experimental models. Data in panel A and D are shown as mean ± SEM and in panel B as box and whisker (Tukey distribution).
    Mouse Il 6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse il 6/product/R&D Systems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse il 6 - by Bioz Stars, 2021-05
    99/100 stars
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    97
    PeproTech rmil 6
    Lack of effect of <t>IL‐6</t> on GLP‐1 Secretion in the Perfused Mouse Small Intestine and in GLP‐1 producing GLUTag cells. (A) GLP‐1 secretion (pmol/L) in perfused mouse small intestines ( n = 6). Infusion of IL‐6 (100 ng/mL) (10–20 min), followed by a period in the absence of IL‐6 (21–39 min), and finally, a period in the presence of 10 nmol/L of bombesin (positive control; 40–45 min). (B) Hormone output (secretion) calculated as area under the curve, with (gray) or without (white) IL‐6 (100 ng/mL), was calculated for each mouse and illustrated collectively as bar and whiskers (Tukey distribution). (C) IL‐6 bioactivity in perfusion samples (#1‐#4) was determined by the ability of these perfusates to induce STAT3 phosphorylation (P‐STAT3) as analyzed by SDS‐PAGE and Western blotting. One‐hundred ng/mL of mIL‐6 was used as positive control (pos.) and Krebs‐Ringer bicarbonate buffer as negative control (neg.). Total STAT3 and α ‐tubulin were used as loading controls. (D) GLP‐1 levels in cell media after 2 h incubation with buffer, 100 or 1000 ng/mL IL‐6, or 10 mmol/L glucose ( n = 4). IL‐6 had no effect on GLP‐1 secretion compared to basal levels in either of these experimental models. Data in panel A and D are shown as mean ± SEM and in panel B as box and whisker (Tukey distribution).
    Rmil 6, supplied by PeproTech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
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    94
    Thermo Fisher mouse il 6
    Anti-IL-6R reduces the expression of IFN-γ in activated Xiap −/− Treg cells. a , b Anti-IL-6R decreases IFN-γ expression in re-stimulated Xiap −/− Treg cells. WT and Xiap −/− iTreg cells were stimulated with anti-CD3/CD28 and IL-2, or with additional IL-12 as indicated, in the absence or presence of anti-IL-6R (50 μg ml −1 ) for 4 days. iTreg cells were re-stimulated with TPA/A23187 for 24 h and IFN-γ production was determined by ELISA ( a ), or reactivated with TPA/A23187 for 5 h and the expressions of Foxp3 and IFN-γ were determined by intracellular staining ( b ). c , d Anti-IL-6R inhibits IFN-γ expression in human Treg cells. Control and human XIAP-knockdown iTreg cells were stimulated as in ( a , b ), with additional <t>IL-6</t> as indicated, and secretion ( c ) or intracellular expression ( d ) of IFN-γ was determined. e Inability of anti-TNF or anti-IL-1R to inhibit the production of IFN-γ in activated Xiap −/− Treg cells. WT and Xiap −/− iTreg cells were stimulated, as described in ( a ), in the presence or absence of anti-TNF or anti-IL-1R (50 μg ml −1 each) for 4 days. Treg cells were re-stimulated with TPA/A23187 for 24 h and the secreted IFN-γ was determined by ELISA. f Anti-IL-6R rescues the impaired suppressive activity of Xiap −/− tTreg cells in vivo. CD45.2 + WT or Xiap −/− tTreg cells were co-transferred with CD45.1 + CD4 + CD25 - effector T cells into male CD45.1 + RagI −/− mice. Anti-IL-6R antibody (500 μg per mouse) was intraperitoneally administrated at day 0, followed by weekly dosing of 500 μg. The body weights of mice were monitored at the indicated time-points. *** P
    Mouse Il 6, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Interleukin 6 IL 6 is an alpha helical cytokine with a wide variety of biological functions including inducement of acute phase reactions inflammation hematopoiesis bone metabolism and cancer progression It
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    Category Kits CLIA Kits Mouse IL 6 Interleukin 6 CLIA Kit Size 96T Price 682 Reactivity Mouse
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    Image Search Results


    Chronic CS exposure increases BAL fluid cellularity and enhances both lung pro‐inflammatory and oxidative stress mediator gene expression. The lungs of mice exposed to CS were lavaged for the assessment of total cells (a), macrophages (b), neutrophils (c), and lymphocytes (d) ( n = 10). Whole lungs excised from mice were then used to measure mRNA expression by RT‐qPCR of TNFα (e) ( n = 10), IL‐6 (f) ( n = 10), and NOX‐2 (g) ( n = 9). Gene expression data are expressed as fold change relative to the sham group. All data are expressed as mean + SEM. * P

    Journal: British Journal of Pharmacology

    Article Title: Ebselen reduces cigarette smoke‐induced endothelial dysfunction in mice, et al. Ebselen reduces cigarette smoke‐induced endothelial dysfunction in mice

    doi: 10.1111/bph.15400

    Figure Lengend Snippet: Chronic CS exposure increases BAL fluid cellularity and enhances both lung pro‐inflammatory and oxidative stress mediator gene expression. The lungs of mice exposed to CS were lavaged for the assessment of total cells (a), macrophages (b), neutrophils (c), and lymphocytes (d) ( n = 10). Whole lungs excised from mice were then used to measure mRNA expression by RT‐qPCR of TNFα (e) ( n = 10), IL‐6 (f) ( n = 10), and NOX‐2 (g) ( n = 9). Gene expression data are expressed as fold change relative to the sham group. All data are expressed as mean + SEM. * P

    Article Snippet: 2.9 Materials The suppliers of the following compounds are as follows: Winfield red cigarettes (Phillip Morris, Australia); ebselen (Sapphire Bioscience, Australia); CM‐cellulose (Sigma‐Aldrich, USA); sodium pentabarbitone (Virbac, Australia); acridine orange/ethidium bromide (Invitrogen, USA); Kwik‐Diff® reagent 1, fixative (Thermo Fisher Scientific, USA); Hemacolour® rapid staining of blood smear (eosin solution) (Merck, USA); Hemacolour® rapid staining of blood smear (thiazine solution) (Merck, USA); RNeasy Mini Kit (Qiagen, Germany); High Capacity RNA‐to‐cDNA kit (Thermo Fisher Scientific, USA); pre‐developed TaqMan primers to: TNF‐α (ID: Mm00443258_m), IL‐6 (ID: Mm00446190_m1), NOX‐2/CYBB (ID: Mm01287743_m1), GPx‐1 (ID: Mm00656767_g1) (Thermo Fisher Scientific, USA); U46619 (Cayman Chemical, USA); ACh (Thermo Fisher, USA); sodium nitroprusside (Thermo Fisher, USA); eNOS/NOS3 RRID: AB_2533121 (Thermo Fisher Scientific, USA), 3‐NT RRID: AB110282 (Abcam, USA); Goat anti‐mouse IgG (H + L) secondary antibody, Alexa Flour Plus 488 RRID: AB_2633275 (Thermo Fisher Scientific, USA); Fluoromount‐G™, with DAPI (Thermo Fisher Scientific, USA).

    Techniques: Expressing, Mouse Assay, Quantitative RT-PCR

    Lack of effect of IL‐6 on GLP‐1 Secretion in the Perfused Mouse Small Intestine and in GLP‐1 producing GLUTag cells. (A) GLP‐1 secretion (pmol/L) in perfused mouse small intestines ( n = 6). Infusion of IL‐6 (100 ng/mL) (10–20 min), followed by a period in the absence of IL‐6 (21–39 min), and finally, a period in the presence of 10 nmol/L of bombesin (positive control; 40–45 min). (B) Hormone output (secretion) calculated as area under the curve, with (gray) or without (white) IL‐6 (100 ng/mL), was calculated for each mouse and illustrated collectively as bar and whiskers (Tukey distribution). (C) IL‐6 bioactivity in perfusion samples (#1‐#4) was determined by the ability of these perfusates to induce STAT3 phosphorylation (P‐STAT3) as analyzed by SDS‐PAGE and Western blotting. One‐hundred ng/mL of mIL‐6 was used as positive control (pos.) and Krebs‐Ringer bicarbonate buffer as negative control (neg.). Total STAT3 and α ‐tubulin were used as loading controls. (D) GLP‐1 levels in cell media after 2 h incubation with buffer, 100 or 1000 ng/mL IL‐6, or 10 mmol/L glucose ( n = 4). IL‐6 had no effect on GLP‐1 secretion compared to basal levels in either of these experimental models. Data in panel A and D are shown as mean ± SEM and in panel B as box and whisker (Tukey distribution).

    Journal: Physiological Reports

    Article Title: Acute administration of interleukin‐6 does not increase secretion of glucagon‐like peptide‐1 in mice. Acute administration of interleukin‐6 does not increase secretion of glucagon‐like peptide‐1 in mice

    doi: 10.14814/phy2.13788

    Figure Lengend Snippet: Lack of effect of IL‐6 on GLP‐1 Secretion in the Perfused Mouse Small Intestine and in GLP‐1 producing GLUTag cells. (A) GLP‐1 secretion (pmol/L) in perfused mouse small intestines ( n = 6). Infusion of IL‐6 (100 ng/mL) (10–20 min), followed by a period in the absence of IL‐6 (21–39 min), and finally, a period in the presence of 10 nmol/L of bombesin (positive control; 40–45 min). (B) Hormone output (secretion) calculated as area under the curve, with (gray) or without (white) IL‐6 (100 ng/mL), was calculated for each mouse and illustrated collectively as bar and whiskers (Tukey distribution). (C) IL‐6 bioactivity in perfusion samples (#1‐#4) was determined by the ability of these perfusates to induce STAT3 phosphorylation (P‐STAT3) as analyzed by SDS‐PAGE and Western blotting. One‐hundred ng/mL of mIL‐6 was used as positive control (pos.) and Krebs‐Ringer bicarbonate buffer as negative control (neg.). Total STAT3 and α ‐tubulin were used as loading controls. (D) GLP‐1 levels in cell media after 2 h incubation with buffer, 100 or 1000 ng/mL IL‐6, or 10 mmol/L glucose ( n = 4). IL‐6 had no effect on GLP‐1 secretion compared to basal levels in either of these experimental models. Data in panel A and D are shown as mean ± SEM and in panel B as box and whisker (Tukey distribution).

    Article Snippet: Test substances consisted of mouse IL‐6 (100 and 1000 ng/mL, R & D Systems, Minneapolis, USA, cat.no.

    Techniques: Positive Control, SDS Page, Western Blot, Negative Control, Incubation, Whisker Assay

    Anti-IL-6R reduces the expression of IFN-γ in activated Xiap −/− Treg cells. a , b Anti-IL-6R decreases IFN-γ expression in re-stimulated Xiap −/− Treg cells. WT and Xiap −/− iTreg cells were stimulated with anti-CD3/CD28 and IL-2, or with additional IL-12 as indicated, in the absence or presence of anti-IL-6R (50 μg ml −1 ) for 4 days. iTreg cells were re-stimulated with TPA/A23187 for 24 h and IFN-γ production was determined by ELISA ( a ), or reactivated with TPA/A23187 for 5 h and the expressions of Foxp3 and IFN-γ were determined by intracellular staining ( b ). c , d Anti-IL-6R inhibits IFN-γ expression in human Treg cells. Control and human XIAP-knockdown iTreg cells were stimulated as in ( a , b ), with additional IL-6 as indicated, and secretion ( c ) or intracellular expression ( d ) of IFN-γ was determined. e Inability of anti-TNF or anti-IL-1R to inhibit the production of IFN-γ in activated Xiap −/− Treg cells. WT and Xiap −/− iTreg cells were stimulated, as described in ( a ), in the presence or absence of anti-TNF or anti-IL-1R (50 μg ml −1 each) for 4 days. Treg cells were re-stimulated with TPA/A23187 for 24 h and the secreted IFN-γ was determined by ELISA. f Anti-IL-6R rescues the impaired suppressive activity of Xiap −/− tTreg cells in vivo. CD45.2 + WT or Xiap −/− tTreg cells were co-transferred with CD45.1 + CD4 + CD25 - effector T cells into male CD45.1 + RagI −/− mice. Anti-IL-6R antibody (500 μg per mouse) was intraperitoneally administrated at day 0, followed by weekly dosing of 500 μg. The body weights of mice were monitored at the indicated time-points. *** P

    Journal: Nature Communications

    Article Title: IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells

    doi: 10.1038/s41467-018-02862-4

    Figure Lengend Snippet: Anti-IL-6R reduces the expression of IFN-γ in activated Xiap −/− Treg cells. a , b Anti-IL-6R decreases IFN-γ expression in re-stimulated Xiap −/− Treg cells. WT and Xiap −/− iTreg cells were stimulated with anti-CD3/CD28 and IL-2, or with additional IL-12 as indicated, in the absence or presence of anti-IL-6R (50 μg ml −1 ) for 4 days. iTreg cells were re-stimulated with TPA/A23187 for 24 h and IFN-γ production was determined by ELISA ( a ), or reactivated with TPA/A23187 for 5 h and the expressions of Foxp3 and IFN-γ were determined by intracellular staining ( b ). c , d Anti-IL-6R inhibits IFN-γ expression in human Treg cells. Control and human XIAP-knockdown iTreg cells were stimulated as in ( a , b ), with additional IL-6 as indicated, and secretion ( c ) or intracellular expression ( d ) of IFN-γ was determined. e Inability of anti-TNF or anti-IL-1R to inhibit the production of IFN-γ in activated Xiap −/− Treg cells. WT and Xiap −/− iTreg cells were stimulated, as described in ( a ), in the presence or absence of anti-TNF or anti-IL-1R (50 μg ml −1 each) for 4 days. Treg cells were re-stimulated with TPA/A23187 for 24 h and the secreted IFN-γ was determined by ELISA. f Anti-IL-6R rescues the impaired suppressive activity of Xiap −/− tTreg cells in vivo. CD45.2 + WT or Xiap −/− tTreg cells were co-transferred with CD45.1 + CD4 + CD25 - effector T cells into male CD45.1 + RagI −/− mice. Anti-IL-6R antibody (500 μg per mouse) was intraperitoneally administrated at day 0, followed by weekly dosing of 500 μg. The body weights of mice were monitored at the indicated time-points. *** P

    Article Snippet: Recombinant mouse IL-2, human IL-2 and mouse IL-6 were purchased from eBioScience.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Staining, Activity Assay, In Vivo, Mouse Assay