Article Title: IL-27 enhances the lymphocyte mediated innate resistance to primary hookworm infection in the lungs
Figure Lengend Snippet: IL-27 regulates inflammatory mediators during primary pulmonary N. brasiliensis infection. ( A ) In vivo comparison of selected cytokines and chemokines of Il27ra −/− mice and wild type control (C57BL/6NJ) mice to primary N. brasiliensis infection (L3 n=500/mouse) in the lungs (BALF) at 2 days after infection. ( B ) C57BL/6J mice administered with rmIL-27 (100 ng/mouse i.p., once daily on day 0 and day 1) or mock control (0.1% BSA in PBS) during N. brasiliensis infection and analyzed for the presence of inflammatory mediators after 2 days p.i in BALF. All data were obtained by multiplexed bead-based assay (Luminex-200). Graphs (A-B) are presented as mean ± SEM, were analyzed by two-tailed t-test and each circle indicates an individual mouse, * P
Article Snippet: A multiplex bead-based immunoassay (Cytokine & Chemokine 26-Plex Mouse ProcartaPlex™ Panel 1, Thermo Fisher Scientific) was used for simultaneous quantification of the following cytokines/chemokines: IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-18, IL-22, IL-23, IL-27, GROα (CXCL-1), IP-10 (CXCL-10), MCP-1 (CCL-2), MCP-3 (CCL-7), MIP-1α (CCL-3), MIP-1β (CCL-4), MIP-2 (CXCL-2), RANTES (CCL-5), Eotaxin (CCL-11), GM-CSF, IFN gamma, and TNF alpha ( ).
Techniques: Infection, In Vivo, Mouse Assay, Bead-based Assay, Luminex, Two Tailed Test