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    r&d systems il 27
    <t>IL-27</t> regulates inflammatory mediators during primary pulmonary N. brasiliensis infection. ( A ) In vivo comparison of selected cytokines and chemokines of Il27ra −/− mice and wild type control (C57BL/6NJ) mice to primary N. brasiliensis infection (L3 n=500/mouse) in the lungs (BALF) at 2 days after infection. ( B ) C57BL/6J mice administered with rmIL-27 (100 ng/mouse i.p., once daily on day 0 and day 1) or mock control (0.1% BSA in PBS) during N. brasiliensis infection and analyzed for the presence of inflammatory mediators after 2 days p.i in BALF. All data were obtained by multiplexed bead-based assay (Luminex-200). Graphs (A-B) are presented as mean ± SEM, were analyzed by two-tailed t-test and each circle indicates an individual mouse, * P
    Il 27, supplied by r&d systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems rmil 27
    <t>IL-27</t> regulates inflammatory mediators during primary pulmonary N. brasiliensis infection. ( A ) In vivo comparison of selected cytokines and chemokines of Il27ra −/− mice and wild type control (C57BL/6NJ) mice to primary N. brasiliensis infection (L3 n=500/mouse) in the lungs (BALF) at 2 days after infection. ( B ) C57BL/6J mice administered with rmIL-27 (100 ng/mouse i.p., once daily on day 0 and day 1) or mock control (0.1% BSA in PBS) during N. brasiliensis infection and analyzed for the presence of inflammatory mediators after 2 days p.i in BALF. All data were obtained by multiplexed bead-based assay (Luminex-200). Graphs (A-B) are presented as mean ± SEM, were analyzed by two-tailed t-test and each circle indicates an individual mouse, * P
    Rmil 27, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rmil 27/product/R&D Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rmil 27 - by Bioz Stars, 2021-05
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    Boster Bio anti mouse
    <t>IL-27</t> regulates inflammatory mediators during primary pulmonary N. brasiliensis infection. ( A ) In vivo comparison of selected cytokines and chemokines of Il27ra −/− mice and wild type control (C57BL/6NJ) mice to primary N. brasiliensis infection (L3 n=500/mouse) in the lungs (BALF) at 2 days after infection. ( B ) C57BL/6J mice administered with rmIL-27 (100 ng/mouse i.p., once daily on day 0 and day 1) or mock control (0.1% BSA in PBS) during N. brasiliensis infection and analyzed for the presence of inflammatory mediators after 2 days p.i in BALF. All data were obtained by multiplexed bead-based assay (Luminex-200). Graphs (A-B) are presented as mean ± SEM, were analyzed by two-tailed t-test and each circle indicates an individual mouse, * P
    Anti Mouse, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore il 27
    IL-27Ralpha expression. ( A ) Volcano plot of differentially expressed genes in T1D patients compared to healthy controls. Analysis of gene arrays was performed on sorted myeloid dendritic cells (mDCs) from T1D patients (n = 20) and healthy controls (n = 10). ( B ) IL-27Ralpha expression validation by RT PCR normalized to GADPH . Analysis was performed on sorted mDCs from 14 T1D patients and 7 healthy donors (HD). Surface expression of IL-27Ralpha on mDC expressed as ( C ) MFI and (D) percentage of IL-27Ralpha + CD11c + mDCs detected by flow cytometry in 9 T1D patients and 6 HD. ( E ) Serum levels of <t>IL-27</t> in 30 T1D patients and 23 healthy donors detected by Luminex. ( F ) Serum levels of soluble IL-27Ralpha in T1D patients (n = 40) and healthy donors (n = 32) detected by ELISA. Statistical analysis was performed using the Mann-Whitney unpaired t -test. Values of p
    Il 27, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 27/product/Millipore
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    N/A
    mIL 27 Recombinant Mouse Interleukin 27 Recombinant Mouse Interleukin 27
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    N/A
    IL 27 A stands for interleukin 27 subunit alpha Mouse IL 27 subunit alpha is a recombinant protein optimized for use in cell culture differentiation studies and functional assays ApplicationsMouse
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    Image Search Results


    IL-27 regulates inflammatory mediators during primary pulmonary N. brasiliensis infection. ( A ) In vivo comparison of selected cytokines and chemokines of Il27ra −/− mice and wild type control (C57BL/6NJ) mice to primary N. brasiliensis infection (L3 n=500/mouse) in the lungs (BALF) at 2 days after infection. ( B ) C57BL/6J mice administered with rmIL-27 (100 ng/mouse i.p., once daily on day 0 and day 1) or mock control (0.1% BSA in PBS) during N. brasiliensis infection and analyzed for the presence of inflammatory mediators after 2 days p.i in BALF. All data were obtained by multiplexed bead-based assay (Luminex-200). Graphs (A-B) are presented as mean ± SEM, were analyzed by two-tailed t-test and each circle indicates an individual mouse, * P

    Journal: bioRxiv

    Article Title: IL-27 enhances the lymphocyte mediated innate resistance to primary hookworm infection in the lungs

    doi: 10.1101/2020.08.12.248021

    Figure Lengend Snippet: IL-27 regulates inflammatory mediators during primary pulmonary N. brasiliensis infection. ( A ) In vivo comparison of selected cytokines and chemokines of Il27ra −/− mice and wild type control (C57BL/6NJ) mice to primary N. brasiliensis infection (L3 n=500/mouse) in the lungs (BALF) at 2 days after infection. ( B ) C57BL/6J mice administered with rmIL-27 (100 ng/mouse i.p., once daily on day 0 and day 1) or mock control (0.1% BSA in PBS) during N. brasiliensis infection and analyzed for the presence of inflammatory mediators after 2 days p.i in BALF. All data were obtained by multiplexed bead-based assay (Luminex-200). Graphs (A-B) are presented as mean ± SEM, were analyzed by two-tailed t-test and each circle indicates an individual mouse, * P

    Article Snippet: A multiplex bead-based immunoassay (Cytokine & Chemokine 26-Plex Mouse ProcartaPlex™ Panel 1, Thermo Fisher Scientific) was used for simultaneous quantification of the following cytokines/chemokines: IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-18, IL-22, IL-23, IL-27, GROα (CXCL-1), IP-10 (CXCL-10), MCP-1 (CCL-2), MCP-3 (CCL-7), MIP-1α (CCL-3), MIP-1β (CCL-4), MIP-2 (CXCL-2), RANTES (CCL-5), Eotaxin (CCL-11), GM-CSF, IFN gamma, and TNF alpha ( ).

    Techniques: Infection, In Vivo, Mouse Assay, Bead-based Assay, Luminex, Two Tailed Test

    IL-27 enhances innate resistance to primary N. brasiliensis infection in the lungs. ( A-C ) In vivo comparison of susceptibility of Il27ra −/− mice and wild type (WT; C57BL/6NJ) mice to primary N. brasiliensis infection (L3 n=500/mouse) in the lungs at 2 days after infection. ( A ) Alveolar parasite burden, ( B ) alveolar hemorrhage, and ( C ) alveolar total protein in BALF. ( D - F ) In vivo comparison of susceptibility of WT mice (C57BL/6J) administered with rmIL-27 (100 ng/mouse i.p., once daily on days 0-1) or mock control (0.1% BSA in PBS) during N. brasiliensis infection and analyzed after 2 days p.i. ( D ) Comparison of alveolar parasite burden, ( E ) alveolar hemorrhage, and ( F ) alveolar total protein in BALF. Data (A-F) are shown as mean ± SEM and each circle indicates an individual mouse, * P

    Journal: bioRxiv

    Article Title: IL-27 enhances the lymphocyte mediated innate resistance to primary hookworm infection in the lungs

    doi: 10.1101/2020.08.12.248021

    Figure Lengend Snippet: IL-27 enhances innate resistance to primary N. brasiliensis infection in the lungs. ( A-C ) In vivo comparison of susceptibility of Il27ra −/− mice and wild type (WT; C57BL/6NJ) mice to primary N. brasiliensis infection (L3 n=500/mouse) in the lungs at 2 days after infection. ( A ) Alveolar parasite burden, ( B ) alveolar hemorrhage, and ( C ) alveolar total protein in BALF. ( D - F ) In vivo comparison of susceptibility of WT mice (C57BL/6J) administered with rmIL-27 (100 ng/mouse i.p., once daily on days 0-1) or mock control (0.1% BSA in PBS) during N. brasiliensis infection and analyzed after 2 days p.i. ( D ) Comparison of alveolar parasite burden, ( E ) alveolar hemorrhage, and ( F ) alveolar total protein in BALF. Data (A-F) are shown as mean ± SEM and each circle indicates an individual mouse, * P

    Article Snippet: A multiplex bead-based immunoassay (Cytokine & Chemokine 26-Plex Mouse ProcartaPlex™ Panel 1, Thermo Fisher Scientific) was used for simultaneous quantification of the following cytokines/chemokines: IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-18, IL-22, IL-23, IL-27, GROα (CXCL-1), IP-10 (CXCL-10), MCP-1 (CCL-2), MCP-3 (CCL-7), MIP-1α (CCL-3), MIP-1β (CCL-4), MIP-2 (CXCL-2), RANTES (CCL-5), Eotaxin (CCL-11), GM-CSF, IFN gamma, and TNF alpha ( ).

    Techniques: Infection, In Vivo, Mouse Assay

    Cytokines and chemokines in brochoalveolar lavage fluids after treatment with recombinant mouse IL-27. In vivo comparison of mediators in BALF from WT mice (C57BL/6J) administered with rmIL-27 (100 ng/mouse i.p.) or mock control (0.1% BSA in PBS) on day 0 and day 1 of N. brasiliensis infection and analyzed after 2 days p.i. Data are shown as mean ± SEM and each circle indicates an individual mouse, two-tailed t-test, * P

    Journal: bioRxiv

    Article Title: IL-27 enhances the lymphocyte mediated innate resistance to primary hookworm infection in the lungs

    doi: 10.1101/2020.08.12.248021

    Figure Lengend Snippet: Cytokines and chemokines in brochoalveolar lavage fluids after treatment with recombinant mouse IL-27. In vivo comparison of mediators in BALF from WT mice (C57BL/6J) administered with rmIL-27 (100 ng/mouse i.p.) or mock control (0.1% BSA in PBS) on day 0 and day 1 of N. brasiliensis infection and analyzed after 2 days p.i. Data are shown as mean ± SEM and each circle indicates an individual mouse, two-tailed t-test, * P

    Article Snippet: A multiplex bead-based immunoassay (Cytokine & Chemokine 26-Plex Mouse ProcartaPlex™ Panel 1, Thermo Fisher Scientific) was used for simultaneous quantification of the following cytokines/chemokines: IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-18, IL-22, IL-23, IL-27, GROα (CXCL-1), IP-10 (CXCL-10), MCP-1 (CCL-2), MCP-3 (CCL-7), MIP-1α (CCL-3), MIP-1β (CCL-4), MIP-2 (CXCL-2), RANTES (CCL-5), Eotaxin (CCL-11), GM-CSF, IFN gamma, and TNF alpha ( ).

    Techniques: Recombinant, In Vivo, Mouse Assay, Infection, Two Tailed Test

    IL-27Ralpha expression. ( A ) Volcano plot of differentially expressed genes in T1D patients compared to healthy controls. Analysis of gene arrays was performed on sorted myeloid dendritic cells (mDCs) from T1D patients (n = 20) and healthy controls (n = 10). ( B ) IL-27Ralpha expression validation by RT PCR normalized to GADPH . Analysis was performed on sorted mDCs from 14 T1D patients and 7 healthy donors (HD). Surface expression of IL-27Ralpha on mDC expressed as ( C ) MFI and (D) percentage of IL-27Ralpha + CD11c + mDCs detected by flow cytometry in 9 T1D patients and 6 HD. ( E ) Serum levels of IL-27 in 30 T1D patients and 23 healthy donors detected by Luminex. ( F ) Serum levels of soluble IL-27Ralpha in T1D patients (n = 40) and healthy donors (n = 32) detected by ELISA. Statistical analysis was performed using the Mann-Whitney unpaired t -test. Values of p

    Journal: Scientific Reports

    Article Title: Enhanced STAT3 phosphorylation and PD-L1 expression in myeloid dendritic cells indicate impaired IL-27Ralpha signaling in type 1 diabetes

    doi: 10.1038/s41598-020-57507-8

    Figure Lengend Snippet: IL-27Ralpha expression. ( A ) Volcano plot of differentially expressed genes in T1D patients compared to healthy controls. Analysis of gene arrays was performed on sorted myeloid dendritic cells (mDCs) from T1D patients (n = 20) and healthy controls (n = 10). ( B ) IL-27Ralpha expression validation by RT PCR normalized to GADPH . Analysis was performed on sorted mDCs from 14 T1D patients and 7 healthy donors (HD). Surface expression of IL-27Ralpha on mDC expressed as ( C ) MFI and (D) percentage of IL-27Ralpha + CD11c + mDCs detected by flow cytometry in 9 T1D patients and 6 HD. ( E ) Serum levels of IL-27 in 30 T1D patients and 23 healthy donors detected by Luminex. ( F ) Serum levels of soluble IL-27Ralpha in T1D patients (n = 40) and healthy donors (n = 32) detected by ELISA. Statistical analysis was performed using the Mann-Whitney unpaired t -test. Values of p

    Article Snippet: IL-27 and soluble IL-27Ralpha serum levels The concentration of sIL-27Ralpha in T1D sera was determined by ELISA (Sigma Aldrich, St. Luis, USA) following the manufacturer’s instructions.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Flow Cytometry, Cytometry, Luminex, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    IL-27 signaling. ( A ) STAT1 (Tyr701) and STAT3 (Tyr705) phosphorylation in myeloid dendritic cells (mDCs) was analyzed by phosphoflow after whole blood from T1D patients (n = 11), and healthy donors (HD, n = 11) was stimulated with 100 ng/ml rhIL-27 for 15 minutes. Values are expressed as MFI (mean fluorescence intensity). ( B ) Expression of STAT1-induced CXCL10 and STAT3-induced SOCS3 after rhIL-27 (100 ng/ml) exposure in T1D patients (n = 16) and healthy donors PBMCs (n = 9) was normalized to GADPH and determined by RT PCR. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s multiple comparisons, Wilcoxon paired or Mann-Whitney unpaired t -test. Values of p

    Journal: Scientific Reports

    Article Title: Enhanced STAT3 phosphorylation and PD-L1 expression in myeloid dendritic cells indicate impaired IL-27Ralpha signaling in type 1 diabetes

    doi: 10.1038/s41598-020-57507-8

    Figure Lengend Snippet: IL-27 signaling. ( A ) STAT1 (Tyr701) and STAT3 (Tyr705) phosphorylation in myeloid dendritic cells (mDCs) was analyzed by phosphoflow after whole blood from T1D patients (n = 11), and healthy donors (HD, n = 11) was stimulated with 100 ng/ml rhIL-27 for 15 minutes. Values are expressed as MFI (mean fluorescence intensity). ( B ) Expression of STAT1-induced CXCL10 and STAT3-induced SOCS3 after rhIL-27 (100 ng/ml) exposure in T1D patients (n = 16) and healthy donors PBMCs (n = 9) was normalized to GADPH and determined by RT PCR. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s multiple comparisons, Wilcoxon paired or Mann-Whitney unpaired t -test. Values of p

    Article Snippet: IL-27 and soluble IL-27Ralpha serum levels The concentration of sIL-27Ralpha in T1D sera was determined by ELISA (Sigma Aldrich, St. Luis, USA) following the manufacturer’s instructions.

    Techniques: Fluorescence, Expressing, Reverse Transcription Polymerase Chain Reaction, MANN-WHITNEY