mouse il-23 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    R&D Systems il 23
    SR3335 modulates human memory T H 17 and T H 17.1 cells. a Naive human CD4 + T cells were differentiated under T H 17 polarizing conditions (anti-CD3 + <t>IL-23+IL-1β)</t> and treated with vehicle (DMSO) or SR3335. IL-17A and IFNγ expression were analyzed from live, CD45RO + cells by flow cytometry on Day 6. Graphs indicate percent IL-17A + cells and frequency of live cells in cultures with compound treatment. b FACS sorted memory cell (CD4 + CD25 − CD45RO + ) subsets from healthy adult donor peripheral blood were stimulated with PMA and ionomycin to determine cytokine production by flow cytometry after 6 days in culture in the presence of vehicle (V, DMSO) or SR3335 (SR, 5μM). Graphs depict percentage cytokine expression (IL-17A, IL-13, and IFNγ) in the cultures for each subset/sample. FACS analysis of T H 17 and T H 17.1 sorted memory cells to determine c IL-17A and IL-22 expression and d TNFα expression. e Graphs summarizing the percentage TNFα expression in sorted T H 1 and T H 2 memory cells treated with vehicle (V, DMSO) or SR3335 (SR, 5 μM) for 6 days from four different donors. f Nanostring data demonstrating the SR3335-mediated changes in gene expression in T H 17 and T H 17.1 cultures on day 6 from the combined data of three donors described in panel e . Data are presented as mean values ± s.e.m. Student’s two-tailed t tests were performed for statistical analysis. Source data are provided as a Source Data file.
    Il 23, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 23/product/R&D Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 23 - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson il 12p40
    <t>IL-12p40</t> production by BM-DCs stimulated with synthesized ODNs based on the URA5 gene. There were several ODNs of 18 to 24 bp found in URA5 DNA that induced IL-12p40 production by BM-DCs. (A and B) BM-DCs were cultured with Cap67 DNA (10 μg/ml), the URA5 PCR product from Cap67 (10 μg/ml), various ODNs (10 μg/ml each of ODN1, ODN2, and ODN3 and 30 μg/ml each of ODN11, ODN12, and ODN13), CpG1826 (1 μg/ml), or LPS (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. (C) BM-DCs from WT (solid columns) or TLR9KO (open columns) mice were cultured with Cap67 DNA (10 μg/ml), the URA5 PCR product from Cap67 (10 μg/ml), ODN111, -112, -113, -121, -122, or -123 (30 μg/ml), CpG1826 (1 μg/ml), or LPS (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. Cn-DNA, Cap67 DNA; ODN, synthesized oligodeoxynucleotide; CpG, CpG1826. *, P
    Il 12p40, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 12p40/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 12p40 - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    98
    R&D Systems recombinant mouse il 23
    <t>IL-12p40</t> production by BM-DCs stimulated with synthesized ODNs based on the URA5 gene. There were several ODNs of 18 to 24 bp found in URA5 DNA that induced IL-12p40 production by BM-DCs. (A and B) BM-DCs were cultured with Cap67 DNA (10 μg/ml), the URA5 PCR product from Cap67 (10 μg/ml), various ODNs (10 μg/ml each of ODN1, ODN2, and ODN3 and 30 μg/ml each of ODN11, ODN12, and ODN13), CpG1826 (1 μg/ml), or LPS (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. (C) BM-DCs from WT (solid columns) or TLR9KO (open columns) mice were cultured with Cap67 DNA (10 μg/ml), the URA5 PCR product from Cap67 (10 μg/ml), ODN111, -112, -113, -121, -122, or -123 (30 μg/ml), CpG1826 (1 μg/ml), or LPS (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. Cn-DNA, Cap67 DNA; ODN, synthesized oligodeoxynucleotide; CpG, CpG1826. *, P
    Recombinant Mouse Il 23, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant mouse il 23/product/R&D Systems
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant mouse il 23 - by Bioz Stars, 2021-05
    98/100 stars
      Buy from Supplier

    95
    PeproTech il 23
    <t>IL-12p40</t> production by BM-DCs stimulated with synthesized ODNs based on the URA5 gene. There were several ODNs of 18 to 24 bp found in URA5 DNA that induced IL-12p40 production by BM-DCs. (A and B) BM-DCs were cultured with Cap67 DNA (10 μg/ml), the URA5 PCR product from Cap67 (10 μg/ml), various ODNs (10 μg/ml each of ODN1, ODN2, and ODN3 and 30 μg/ml each of ODN11, ODN12, and ODN13), CpG1826 (1 μg/ml), or LPS (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. (C) BM-DCs from WT (solid columns) or TLR9KO (open columns) mice were cultured with Cap67 DNA (10 μg/ml), the URA5 PCR product from Cap67 (10 μg/ml), ODN111, -112, -113, -121, -122, or -123 (30 μg/ml), CpG1826 (1 μg/ml), or LPS (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. Cn-DNA, Cap67 DNA; ODN, synthesized oligodeoxynucleotide; CpG, CpG1826. *, P
    Il 23, supplied by PeproTech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 23/product/PeproTech
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 23 - by Bioz Stars, 2021-05
    95/100 stars
      Buy from Supplier


    N/A
    Interleukin 23 IL 23 is a heterodimeric cytokine composed of the IL 12 p40 “soluble receptor subunit and a novel cytokine like subunit related to IL 12 p35 termed p19
      Buy from Supplier


    N/A
    Category Kits ELISA Kits Mouse IL 23 Interleukin 23 ELISA Kit Size 96T Price 609 Reactivity Mouse Uniprot Id Q9EQ14
      Buy from Supplier

    Image Search Results


    SR3335 modulates human memory T H 17 and T H 17.1 cells. a Naive human CD4 + T cells were differentiated under T H 17 polarizing conditions (anti-CD3 + IL-23+IL-1β) and treated with vehicle (DMSO) or SR3335. IL-17A and IFNγ expression were analyzed from live, CD45RO + cells by flow cytometry on Day 6. Graphs indicate percent IL-17A + cells and frequency of live cells in cultures with compound treatment. b FACS sorted memory cell (CD4 + CD25 − CD45RO + ) subsets from healthy adult donor peripheral blood were stimulated with PMA and ionomycin to determine cytokine production by flow cytometry after 6 days in culture in the presence of vehicle (V, DMSO) or SR3335 (SR, 5μM). Graphs depict percentage cytokine expression (IL-17A, IL-13, and IFNγ) in the cultures for each subset/sample. FACS analysis of T H 17 and T H 17.1 sorted memory cells to determine c IL-17A and IL-22 expression and d TNFα expression. e Graphs summarizing the percentage TNFα expression in sorted T H 1 and T H 2 memory cells treated with vehicle (V, DMSO) or SR3335 (SR, 5 μM) for 6 days from four different donors. f Nanostring data demonstrating the SR3335-mediated changes in gene expression in T H 17 and T H 17.1 cultures on day 6 from the combined data of three donors described in panel e . Data are presented as mean values ± s.e.m. Student’s two-tailed t tests were performed for statistical analysis. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Genetic and pharmacological inhibition of the nuclear receptor RORα regulates TH17 driven inflammatory disorders

    doi: 10.1038/s41467-020-20385-9

    Figure Lengend Snippet: SR3335 modulates human memory T H 17 and T H 17.1 cells. a Naive human CD4 + T cells were differentiated under T H 17 polarizing conditions (anti-CD3 + IL-23+IL-1β) and treated with vehicle (DMSO) or SR3335. IL-17A and IFNγ expression were analyzed from live, CD45RO + cells by flow cytometry on Day 6. Graphs indicate percent IL-17A + cells and frequency of live cells in cultures with compound treatment. b FACS sorted memory cell (CD4 + CD25 − CD45RO + ) subsets from healthy adult donor peripheral blood were stimulated with PMA and ionomycin to determine cytokine production by flow cytometry after 6 days in culture in the presence of vehicle (V, DMSO) or SR3335 (SR, 5μM). Graphs depict percentage cytokine expression (IL-17A, IL-13, and IFNγ) in the cultures for each subset/sample. FACS analysis of T H 17 and T H 17.1 sorted memory cells to determine c IL-17A and IL-22 expression and d TNFα expression. e Graphs summarizing the percentage TNFα expression in sorted T H 1 and T H 2 memory cells treated with vehicle (V, DMSO) or SR3335 (SR, 5 μM) for 6 days from four different donors. f Nanostring data demonstrating the SR3335-mediated changes in gene expression in T H 17 and T H 17.1 cultures on day 6 from the combined data of three donors described in panel e . Data are presented as mean values ± s.e.m. Student’s two-tailed t tests were performed for statistical analysis. Source data are provided as a Source Data file.

    Article Snippet: Other cytokines used for various TH 17 conditions: IL-1β (10 ng/ml, R & D Systems) and IL-23 (20 ng/ml, R & D Systems).

    Techniques: Expressing, Flow Cytometry, FACS, Two Tailed Test

    IL-12p40 production by BM-DCs stimulated with synthesized ODNs based on the URA5 gene. There were several ODNs of 18 to 24 bp found in URA5 DNA that induced IL-12p40 production by BM-DCs. (A and B) BM-DCs were cultured with Cap67 DNA (10 μg/ml), the URA5 PCR product from Cap67 (10 μg/ml), various ODNs (10 μg/ml each of ODN1, ODN2, and ODN3 and 30 μg/ml each of ODN11, ODN12, and ODN13), CpG1826 (1 μg/ml), or LPS (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. (C) BM-DCs from WT (solid columns) or TLR9KO (open columns) mice were cultured with Cap67 DNA (10 μg/ml), the URA5 PCR product from Cap67 (10 μg/ml), ODN111, -112, -113, -121, -122, or -123 (30 μg/ml), CpG1826 (1 μg/ml), or LPS (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. Cn-DNA, Cap67 DNA; ODN, synthesized oligodeoxynucleotide; CpG, CpG1826. *, P

    Journal: Infection and Immunity

    Article Title: Toll-Like Receptor 9-Dependent Activation of Bone Marrow-Derived Dendritic Cells by URA5 DNA from Cryptococcus neoformans

    doi: 10.1128/IAI.05570-11

    Figure Lengend Snippet: IL-12p40 production by BM-DCs stimulated with synthesized ODNs based on the URA5 gene. There were several ODNs of 18 to 24 bp found in URA5 DNA that induced IL-12p40 production by BM-DCs. (A and B) BM-DCs were cultured with Cap67 DNA (10 μg/ml), the URA5 PCR product from Cap67 (10 μg/ml), various ODNs (10 μg/ml each of ODN1, ODN2, and ODN3 and 30 μg/ml each of ODN11, ODN12, and ODN13), CpG1826 (1 μg/ml), or LPS (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. (C) BM-DCs from WT (solid columns) or TLR9KO (open columns) mice were cultured with Cap67 DNA (10 μg/ml), the URA5 PCR product from Cap67 (10 μg/ml), ODN111, -112, -113, -121, -122, or -123 (30 μg/ml), CpG1826 (1 μg/ml), or LPS (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. Cn-DNA, Cap67 DNA; ODN, synthesized oligodeoxynucleotide; CpG, CpG1826. *, P

    Article Snippet: The concentrations of IL-12p40 in the culture supernatants were measured by an enzyme-linked immunosorbent assay (ELISA) using capture and biotinylated developing antibodies (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Synthesized, Cell Culture, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Effects of phosphorothioate modification and DOTAP on BM-DC production of IL-12p40. ODN112 and CpG1826 were distinct in terms of the effects of PS modification and DOTAP treatment on their ability to induce production of IL-12p40 by BM-DCs, whereas uptake of these ODNs by BM-DCs was equivalent. (A) BM-DCs were cultured with PO-ODN112 (30 μg/ml), PS-ODN112 (30 μg/ml), PO-CpG1826 (1 μg/ml), or PS-CpG1826 (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. (B) BM-DCs were cultured with PO-ODN112 (30 μg/ml), PO-ODN123 (30 μg/ml), PS-ODN112 (30 μg/ml), PO-ODN122 (30 μg/ml), PO-ODN122-GC (30 μg/ml), or PS-CpG1826 (0.01, 0.1, or 1 μg/ml) in the presence (open columns) or absence (solid columns) of DOTAP for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. PO, phosphodiester; PS, phosphorothioate; ODN, synthesized oligodeoxynucleotide DNA; CpG, CpG1826. *, P

    Journal: Infection and Immunity

    Article Title: Toll-Like Receptor 9-Dependent Activation of Bone Marrow-Derived Dendritic Cells by URA5 DNA from Cryptococcus neoformans

    doi: 10.1128/IAI.05570-11

    Figure Lengend Snippet: Effects of phosphorothioate modification and DOTAP on BM-DC production of IL-12p40. ODN112 and CpG1826 were distinct in terms of the effects of PS modification and DOTAP treatment on their ability to induce production of IL-12p40 by BM-DCs, whereas uptake of these ODNs by BM-DCs was equivalent. (A) BM-DCs were cultured with PO-ODN112 (30 μg/ml), PS-ODN112 (30 μg/ml), PO-CpG1826 (1 μg/ml), or PS-CpG1826 (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. (B) BM-DCs were cultured with PO-ODN112 (30 μg/ml), PO-ODN123 (30 μg/ml), PS-ODN112 (30 μg/ml), PO-ODN122 (30 μg/ml), PO-ODN122-GC (30 μg/ml), or PS-CpG1826 (0.01, 0.1, or 1 μg/ml) in the presence (open columns) or absence (solid columns) of DOTAP for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. PO, phosphodiester; PS, phosphorothioate; ODN, synthesized oligodeoxynucleotide DNA; CpG, CpG1826. *, P

    Article Snippet: The concentrations of IL-12p40 in the culture supernatants were measured by an enzyme-linked immunosorbent assay (ELISA) using capture and biotinylated developing antibodies (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Modification, Cell Culture, Enzyme-linked Immunosorbent Assay, Synthesized

    CD40 expression by BM-DCs stimulated with synthesized ODNs based on the URA5 gene. ODNs with the ability to induce IL-12p40 production were also active in the expression of CD40 by BM-DCs. BM-DCs were cultured with Cap67 DNA (10 μg/ml), ODN111, -112, -113, -121, -122, or -123 (30 μg/ml), or CpG1826 (1 μg/ml) for 24 h, and CD40 expression by BM-DCs was measured using a flow cytometer. Representative data for three independent experiments are shown. The percentages indicate the proportions of positive cells. Cn-DNA, Cap67; ODN, synthesized oligodeoxynucleotide; CpG, CpG1826.

    Journal: Infection and Immunity

    Article Title: Toll-Like Receptor 9-Dependent Activation of Bone Marrow-Derived Dendritic Cells by URA5 DNA from Cryptococcus neoformans

    doi: 10.1128/IAI.05570-11

    Figure Lengend Snippet: CD40 expression by BM-DCs stimulated with synthesized ODNs based on the URA5 gene. ODNs with the ability to induce IL-12p40 production were also active in the expression of CD40 by BM-DCs. BM-DCs were cultured with Cap67 DNA (10 μg/ml), ODN111, -112, -113, -121, -122, or -123 (30 μg/ml), or CpG1826 (1 μg/ml) for 24 h, and CD40 expression by BM-DCs was measured using a flow cytometer. Representative data for three independent experiments are shown. The percentages indicate the proportions of positive cells. Cn-DNA, Cap67; ODN, synthesized oligodeoxynucleotide; CpG, CpG1826.

    Article Snippet: The concentrations of IL-12p40 in the culture supernatants were measured by an enzyme-linked immunosorbent assay (ELISA) using capture and biotinylated developing antibodies (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Expressing, Synthesized, Cell Culture, Flow Cytometry, Cytometry

    IL-12p40 production by BM-DCs stimulated with URA5 PCR products. PCR-amplified URA5 DNA and its 5′ 129-bp fragment induce IL-12p40 production by BM-DCs. (A) The PCR product of the URA5 gene was amplified from Cap67 and subjected to electrophoresis. The arrow indicates the 345-bp product. (B) BM-DCs from WT (solid columns) or TLR9KO (open columns) mice were cultured with Cap67 DNA (10 μg/ml), the URA5 PCR product from Cap67 (10 μg/ml), CpG1826 (1 μg/ml), or LPS (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. (C) BM-DCs from WT (solid columns) or TLR9KO (open columns) mice were cultured with URA5 PCR products from strains Cap67, YC-11, and YC-13 (10 μg/ml for each) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. (D) PCR products of 5′, 3′, and intervening fragments of the URA5 gene were amplified from Cap67 and subjected to electrophoresis. Their sizes were 129, 122, and 133 bp, respectively. Each arrow indicates a PCR product. (E) BM-DCs were cultured with PCR products of the URA5 gene from Cap67 (10 μg/ml) and each fragment (10 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. M, 100-bp DNA size marker; 5′-129bp, 5′ PCR product of 129 bp; 3′-133bp, 3′ PCR product of 133 bp; Int-122bp, intervening PCR product of 122 bp; Cn-DNA, Cap67 DNA; CpG, CpG1826. *, P

    Journal: Infection and Immunity

    Article Title: Toll-Like Receptor 9-Dependent Activation of Bone Marrow-Derived Dendritic Cells by URA5 DNA from Cryptococcus neoformans

    doi: 10.1128/IAI.05570-11

    Figure Lengend Snippet: IL-12p40 production by BM-DCs stimulated with URA5 PCR products. PCR-amplified URA5 DNA and its 5′ 129-bp fragment induce IL-12p40 production by BM-DCs. (A) The PCR product of the URA5 gene was amplified from Cap67 and subjected to electrophoresis. The arrow indicates the 345-bp product. (B) BM-DCs from WT (solid columns) or TLR9KO (open columns) mice were cultured with Cap67 DNA (10 μg/ml), the URA5 PCR product from Cap67 (10 μg/ml), CpG1826 (1 μg/ml), or LPS (1 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. (C) BM-DCs from WT (solid columns) or TLR9KO (open columns) mice were cultured with URA5 PCR products from strains Cap67, YC-11, and YC-13 (10 μg/ml for each) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. (D) PCR products of 5′, 3′, and intervening fragments of the URA5 gene were amplified from Cap67 and subjected to electrophoresis. Their sizes were 129, 122, and 133 bp, respectively. Each arrow indicates a PCR product. (E) BM-DCs were cultured with PCR products of the URA5 gene from Cap67 (10 μg/ml) and each fragment (10 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. M, 100-bp DNA size marker; 5′-129bp, 5′ PCR product of 129 bp; 3′-133bp, 3′ PCR product of 133 bp; Int-122bp, intervening PCR product of 122 bp; Cn-DNA, Cap67 DNA; CpG, CpG1826. *, P

    Article Snippet: The concentrations of IL-12p40 in the culture supernatants were measured by an enzyme-linked immunosorbent assay (ELISA) using capture and biotinylated developing antibodies (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Polymerase Chain Reaction, Amplification, Electrophoresis, Mouse Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Marker

    Effect of inactive ODN on BM-DC production of IL-12p40. Excessive doses of ODN123 inhibited IL-12p40 production by BM-DCs stimulated with ODN112 but not by BM-DCs stimulated with CpG1826. BM-DCs were cultured with ODN112 (30 μg/ml) or CpG1826 (1 μg/ml) in the presence or absence of ODN123 (300 μg/ml), and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. ODN, synthesized oligodeoxynucleotide DNA; CpG, CpG1826. *, P

    Journal: Infection and Immunity

    Article Title: Toll-Like Receptor 9-Dependent Activation of Bone Marrow-Derived Dendritic Cells by URA5 DNA from Cryptococcus neoformans

    doi: 10.1128/IAI.05570-11

    Figure Lengend Snippet: Effect of inactive ODN on BM-DC production of IL-12p40. Excessive doses of ODN123 inhibited IL-12p40 production by BM-DCs stimulated with ODN112 but not by BM-DCs stimulated with CpG1826. BM-DCs were cultured with ODN112 (30 μg/ml) or CpG1826 (1 μg/ml) in the presence or absence of ODN123 (300 μg/ml), and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. ODN, synthesized oligodeoxynucleotide DNA; CpG, CpG1826. *, P

    Article Snippet: The concentrations of IL-12p40 in the culture supernatants were measured by an enzyme-linked immunosorbent assay (ELISA) using capture and biotinylated developing antibodies (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Synthesized

    IL-12p40 production by BM-DCs stimulated with altered ODNs of the URA5 gene. Some modified ODN derivatives lost the ability to induce IL-12p40 production by BM-DCs. BM-DCs were cultured with various altered ODNs (30 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. ODN, synthesized oligodeoxynucleotide. *, P

    Journal: Infection and Immunity

    Article Title: Toll-Like Receptor 9-Dependent Activation of Bone Marrow-Derived Dendritic Cells by URA5 DNA from Cryptococcus neoformans

    doi: 10.1128/IAI.05570-11

    Figure Lengend Snippet: IL-12p40 production by BM-DCs stimulated with altered ODNs of the URA5 gene. Some modified ODN derivatives lost the ability to induce IL-12p40 production by BM-DCs. BM-DCs were cultured with various altered ODNs (30 μg/ml) for 24 h, and IL-12p40 concentrations in the culture supernatants were measured by ELISA. Data are means ± SD for triplicate cultures. ODN, synthesized oligodeoxynucleotide. *, P

    Article Snippet: The concentrations of IL-12p40 in the culture supernatants were measured by an enzyme-linked immunosorbent assay (ELISA) using capture and biotinylated developing antibodies (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Modification, Cell Culture, Enzyme-linked Immunosorbent Assay, Synthesized