Journal: Cells

Article Title: Hypothalamic GHR—SIRT1 Axis in Fasting

doi: 10.3390/cells10040891

Figure Lengend Snippet: Weight and hormonal levels of agouti-related protein (AgRP) EYFP∆GHR mice. ( A ) Lean mass, ( B ) fat mass, and ( C ) food intake of control and AgRP EYFP∆GHR male mice. ( D ) Lean mass, ( E ) fat mass, and ( F ) food intake of control and AgRP EYFP∆GHR female mice. ( G ) Serum growth hormone (GH), ( H ) serum IGF-1, and ( I ) fasted blood glucose levels of control and AgRP EYFP∆GHR male mice, n = 7–8 per group. ( J ) Representative image of STAT5-IR (red) in the ARC of control and AgRP EYFP∆GHR male mice induced by saline or GH (ip). ( K ) Quantification of pSTAT5 + cells in the ARC of control and AgRP EYFP∆GHR mice. Mean ± SEM. *, p < 0.05. n = 4–5. The 3V, third ventricle.

Article Snippet: GH levels were determined on fasted mice by the Rat/Mouse Growth Hormone ELISA kit (Millipore, Burlington, MA, USA), and IGF-1 levels were determined by Mouse/Rat IGF-1 ELISA kit (22IG1MSE01, Alpco, Salem, NH, USA).

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Insulin-like growth factor-II overexpression accelerates parthenogenetic stem cell differentiation into cardiomyocytes and improves cardiac function after acute myocardial infarction in mice

doi: 10.1186/s13287-020-1575-4

Figure Lengend Snippet: IGF-II overexpression promotes cardiomyocyte maturation in vivo. ESC- and PSC-derived cardiomyocytes at 1 × 10 5 cells/recipient mouse were injected into the heart at 1 week after acute myocardial infarction (MI). The IGF-II and VEGF levels in the injection sites ( a ) and the serum ( b ) were measured by ELISA at 4 weeks post-transplant. Data are expressed as the mean ± SD. * p < 0.05 vs. any other group. n = 12. c Immunofluorescence staining was performed to observe the cardiomyocyte morphology and to detect α-actinin expression (red staining) in the injection sites at 4 weeks post-transplant. Blue staining, Hoechst 33342. Green staining, eGFP. Scale bars, 10 μm

Article Snippet: IGF-II levels in serum (Quantikine ELISA Kit, cat# MG200, R&D Systems, Minneapolis, MN, USA), heart tissue (DuoSet ELISA, cat# DY792, R&D Systems), vascular endothelial growth factor (VEGF) levels in serum (cat# ab100751; Abcam), and heart tissue (cat# ab209882; Abcam) were determined with ELISA kits following the manufacturer’s instructions.

Techniques: Over Expression, In Vivo, Derivative Assay, Injection, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Expressing