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  • 99
    Thermo Fisher ifn γ
    Cytokine production from human whole blood stimulated by liposomes containing GLA, 3M-052, both TLR ligands, or neither (empty). Stimulated whole blood was analyzed for production of Th1-associated cytokines a IL-12p70 and b <t>IFN-γ,</t> as well as chemokines c Mip-1β and d MCP-1. The x -axes represent serial dilutions with starting concentrations of 4 µg/ml for GLA and 1.5 µg/ml 3M-052. The y -axes represent concentrations of target analytes secreted post whole blood stimulation with error bars representing the standard error of the mean from three blood donors with each individual value averaged from duplicate wells
    Ifn γ, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    BioLegend igg1
    Cytokine production from human whole blood stimulated by liposomes containing GLA, 3M-052, both TLR ligands, or neither (empty). Stimulated whole blood was analyzed for production of Th1-associated cytokines a IL-12p70 and b <t>IFN-γ,</t> as well as chemokines c Mip-1β and d MCP-1. The x -axes represent serial dilutions with starting concentrations of 4 µg/ml for GLA and 1.5 µg/ml 3M-052. The y -axes represent concentrations of target analytes secreted post whole blood stimulation with error bars representing the standard error of the mean from three blood donors with each individual value averaged from duplicate wells
    Igg1, supplied by BioLegend, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore ifn γ
    C5aRA reduces the levels of IL-4, <t>IFN-γ,</t> histamine and IgE in mouse serum. Following DNCB application onto the dorsal skin of BALB/c mice for 2 weeks with or without C5aRA (1 μ g) intracutaneous injection, blood was sampled from the posterior vena cava of mice. Levels of IL-4, IFN-γ, histamine and IgE in serum were determined using ELISA kits. Levels of (A) IL-4, (B) IFN-γ, (C) histamine and (D) IgE were significantly increased. The increases in levels of IL-4, IFN-γ, histamine and IgE in serum were significantly attenuated in the mice receiving C5aRA intracutaneous injection. Values are expressed as the mean ± standard deviation (n=5). ** P
    Ifn γ, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
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    99
    Thermo Fisher anti ifn γ
    Anti-CEACAM1 mAb treatment enhances CD8 + T cell expansion and viral control. a Experimental setup. b , c . One group of wild-type (WT) and Ceacam1 –/– mice received anti-CEACAM1 mAb, and another group received an equal amount of isotype antibody on day –1 and day 3, followed by infection with 2 × 10 4 PFU of LCMV-Docile on day 0. b , c Graphs showing percentage of virus-specific Tet-GP33 + CD8 + T cells in blood ( b ) and viral titers in serum ( c ) in indicated groups over the indicated times. Statistical significance is shown between the WT + IgG-treated groups and the WT + α-CC1 mAb-treated groups ( n = 3–6 per group). d Experimental setup. e Wild-type (WT) mice were infected with 2 × 10 6 PFU of LCMV-Docile on day 0. On day 16 after infection, mice were treated with 200 µg anti-CEACAM1 mAb per mouse or with an isotype antibody. e Viral titers in serum (left panel) over the indicated times and in indicated organs (right panel) on day 60 after infection ( n = 4–8 per group). f Experimental setup. g – i Ceacam1 –/– mice were given 1 × 10 4 P14 × CD45.1 WT splenocytes on day –2. One group of these mice was additionally treated with anti-CEACAM1 mAb, and another group was treated with an equal amount of an isotype antibody on day –1 and on day 3, followed by infection with 2 × 10 4 PFU of LCMV-Docile on day 0. Mice were analyzed on day 8 after infection. g Absolute number of transferred Tet-GP33 + CD45.1 + CD8 + T cells in blood and spleen of host Ceacam1 –/– (CD45.2) mice ( n = 5–7 per group). h Intracellular cytokine <t>IFN-γ</t> secretion from transferred P14 × CD45.1 WT CD8 + T cells ( n = 5–7 per group). i Viral titers in the indicated organs of the recipient mice ( n = 5–7 per group). * P
    Anti Ifn γ, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Mouse IFN gamma Antibody from R D Systems is a rat monoclonal antibody to IFN gamma This antibody reacts with mouse The Mouse IFN gamma Antibody has been validated
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    Cytokine production from human whole blood stimulated by liposomes containing GLA, 3M-052, both TLR ligands, or neither (empty). Stimulated whole blood was analyzed for production of Th1-associated cytokines a IL-12p70 and b IFN-γ, as well as chemokines c Mip-1β and d MCP-1. The x -axes represent serial dilutions with starting concentrations of 4 µg/ml for GLA and 1.5 µg/ml 3M-052. The y -axes represent concentrations of target analytes secreted post whole blood stimulation with error bars representing the standard error of the mean from three blood donors with each individual value averaged from duplicate wells

    Journal: NPJ Vaccines

    Article Title: Adjuvant composition and delivery route shape immune response quality and protective efficacy of a recombinant vaccine for Entamoeba histolytica

    doi: 10.1038/s41541-018-0060-x

    Figure Lengend Snippet: Cytokine production from human whole blood stimulated by liposomes containing GLA, 3M-052, both TLR ligands, or neither (empty). Stimulated whole blood was analyzed for production of Th1-associated cytokines a IL-12p70 and b IFN-γ, as well as chemokines c Mip-1β and d MCP-1. The x -axes represent serial dilutions with starting concentrations of 4 µg/ml for GLA and 1.5 µg/ml 3M-052. The y -axes represent concentrations of target analytes secreted post whole blood stimulation with error bars representing the standard error of the mean from three blood donors with each individual value averaged from duplicate wells

    Article Snippet: Samples were incubated for 24 h at 37 °C/5% CO2 in a humidified incubator and plasma supernatants assayed by ELISA for the selected cytokines (Mip-1β [R & D Systems, catalog #DY271]; IL-12p70 [eBioscience, catalog #88-7126-86]; IFN-γ [eBioscience, catalog #88-7316-86]; MCP-1 [eBioscience, catalog #88-7399-88]).

    Techniques:

    Biological activity of adjuvant formulation: importance of PEG length and complementary roles for GLA and 3M-052. Five mice per group were immunized three times with a 2-week interval between immunizations using a mixed intranasal/subcutaneous regimen. Mice were euthanized a week after third immunization and samples collected. a Stool supernatants were diluted 250-fold and anti-LecA IgA titer was determined by ELISA. b Plasma samples were diluted 100,000-fold and titers of anti-LecA IgG1 (black circles) and IgG2a (red circles) subtypes were determined by ELISA. c Intracellular IFN-γ levels were measured using flow cytometry as described (negative values obtained after subtracting values from unstimulated cells were considered zero; the outlier test described below was conducted prior to this data transformation). Error bars represent standard error of the mean. For the analysis of the data in plot a , data were analyzed by one-way ANOVA with Sidak’s correction for selected comparisons. For the analysis of the data in plot b , data were log-transformed with a small offset if necessary and Sidak’s correction for selected comparisons was employed. All formulations in plot b elicited statistically increased ( p

    Journal: NPJ Vaccines

    Article Title: Adjuvant composition and delivery route shape immune response quality and protective efficacy of a recombinant vaccine for Entamoeba histolytica

    doi: 10.1038/s41541-018-0060-x

    Figure Lengend Snippet: Biological activity of adjuvant formulation: importance of PEG length and complementary roles for GLA and 3M-052. Five mice per group were immunized three times with a 2-week interval between immunizations using a mixed intranasal/subcutaneous regimen. Mice were euthanized a week after third immunization and samples collected. a Stool supernatants were diluted 250-fold and anti-LecA IgA titer was determined by ELISA. b Plasma samples were diluted 100,000-fold and titers of anti-LecA IgG1 (black circles) and IgG2a (red circles) subtypes were determined by ELISA. c Intracellular IFN-γ levels were measured using flow cytometry as described (negative values obtained after subtracting values from unstimulated cells were considered zero; the outlier test described below was conducted prior to this data transformation). Error bars represent standard error of the mean. For the analysis of the data in plot a , data were analyzed by one-way ANOVA with Sidak’s correction for selected comparisons. For the analysis of the data in plot b , data were log-transformed with a small offset if necessary and Sidak’s correction for selected comparisons was employed. All formulations in plot b elicited statistically increased ( p

    Article Snippet: Samples were incubated for 24 h at 37 °C/5% CO2 in a humidified incubator and plasma supernatants assayed by ELISA for the selected cytokines (Mip-1β [R & D Systems, catalog #DY271]; IL-12p70 [eBioscience, catalog #88-7126-86]; IFN-γ [eBioscience, catalog #88-7316-86]; MCP-1 [eBioscience, catalog #88-7399-88]).

    Techniques: Activity Assay, Mouse Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Cytometry, Transformation Assay

    C5aRA reduces the levels of IL-4, IFN-γ, histamine and IgE in mouse serum. Following DNCB application onto the dorsal skin of BALB/c mice for 2 weeks with or without C5aRA (1 μ g) intracutaneous injection, blood was sampled from the posterior vena cava of mice. Levels of IL-4, IFN-γ, histamine and IgE in serum were determined using ELISA kits. Levels of (A) IL-4, (B) IFN-γ, (C) histamine and (D) IgE were significantly increased. The increases in levels of IL-4, IFN-γ, histamine and IgE in serum were significantly attenuated in the mice receiving C5aRA intracutaneous injection. Values are expressed as the mean ± standard deviation (n=5). ** P

    Journal: Molecular Medicine Reports

    Article Title: Role of the complement anaphylatoxin C5a-receptor pathway in atopic dermatitis in mice

    doi: 10.3892/mmr.2015.3301

    Figure Lengend Snippet: C5aRA reduces the levels of IL-4, IFN-γ, histamine and IgE in mouse serum. Following DNCB application onto the dorsal skin of BALB/c mice for 2 weeks with or without C5aRA (1 μ g) intracutaneous injection, blood was sampled from the posterior vena cava of mice. Levels of IL-4, IFN-γ, histamine and IgE in serum were determined using ELISA kits. Levels of (A) IL-4, (B) IFN-γ, (C) histamine and (D) IgE were significantly increased. The increases in levels of IL-4, IFN-γ, histamine and IgE in serum were significantly attenuated in the mice receiving C5aRA intracutaneous injection. Values are expressed as the mean ± standard deviation (n=5). ** P

    Article Snippet: Production of IL-4 and IFN-γ in skin tissue and IL-4, IFN-γ, histamine and IgE levels in serum were determined using ELISA kits (Mouse IL-4, Mouse IFN-γ, Porcine Histamine HIS and Mouse IgE ELISA kits; Sigma-Aldrich) according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Inhibitory mechanism of C5aRA on AD. Following DNCB application onto mouse dorsal skin for 2 weeks, C5aR expression in skin tissue was significantly increased and the infiltration of mast cells was significantly enhanced as well. The activated mast cells released polarizing cytokines to increase IL-4 production from Th2 and IFN-γ production from mixed Th1/Th2. In addition, the increased C5aR expression on mast cells enhanced IgE in B cells. At the same time, mast cells released histamine to generate pruritus symptoms. Intracutaneous injection of C5aRA directly prevented the binding of C5a to C5aR on mast cells, then decreased the skin-fold thickness, number of infiltrating leukocytes and mast cells as well as levels of IL-4, IFN-γ, histamine and IgE, and thereby inhibited the symptoms of AD. AD, atopic dermatitis; DNCB, 2,4-dinitrochlorobenzene; Th, T helper; IL, interleukin; IFN, interferon; IgE, immunoglobulin E; C5aRA, C5a receptor antagonist.

    Journal: Molecular Medicine Reports

    Article Title: Role of the complement anaphylatoxin C5a-receptor pathway in atopic dermatitis in mice

    doi: 10.3892/mmr.2015.3301

    Figure Lengend Snippet: Inhibitory mechanism of C5aRA on AD. Following DNCB application onto mouse dorsal skin for 2 weeks, C5aR expression in skin tissue was significantly increased and the infiltration of mast cells was significantly enhanced as well. The activated mast cells released polarizing cytokines to increase IL-4 production from Th2 and IFN-γ production from mixed Th1/Th2. In addition, the increased C5aR expression on mast cells enhanced IgE in B cells. At the same time, mast cells released histamine to generate pruritus symptoms. Intracutaneous injection of C5aRA directly prevented the binding of C5a to C5aR on mast cells, then decreased the skin-fold thickness, number of infiltrating leukocytes and mast cells as well as levels of IL-4, IFN-γ, histamine and IgE, and thereby inhibited the symptoms of AD. AD, atopic dermatitis; DNCB, 2,4-dinitrochlorobenzene; Th, T helper; IL, interleukin; IFN, interferon; IgE, immunoglobulin E; C5aRA, C5a receptor antagonist.

    Article Snippet: Production of IL-4 and IFN-γ in skin tissue and IL-4, IFN-γ, histamine and IgE levels in serum were determined using ELISA kits (Mouse IL-4, Mouse IFN-γ, Porcine Histamine HIS and Mouse IgE ELISA kits; Sigma-Aldrich) according to the manufacturer’s instructions.

    Techniques: Expressing, Injection, Binding Assay

    C5aRA reduces the increased IL-4 and IFN-γ levels in mouse skin tissue. BALB/c mice were treated with DNCB on dorsal skin for 2 weeks with or without C5aRA (1 μ g) intracutaneous injection. Following DNCB application, the levels of IL-4 and IFN-γ were determined using ELISA kits. (A) Levels of IL-4 in skin tissue were significantly increased. This increase in IL-4 was significantly attenuated in the mice receiving C5aRA intracutaneous injection. (B) Levels of IFN-γ in skin tissue was significantly increased. The increases in IFN-γ levels were significantly attenuated in the mice receiving C5aRA intracutaneous injection. Values are expressed as the mean ± standard deviation (n=5). ** P

    Journal: Molecular Medicine Reports

    Article Title: Role of the complement anaphylatoxin C5a-receptor pathway in atopic dermatitis in mice

    doi: 10.3892/mmr.2015.3301

    Figure Lengend Snippet: C5aRA reduces the increased IL-4 and IFN-γ levels in mouse skin tissue. BALB/c mice were treated with DNCB on dorsal skin for 2 weeks with or without C5aRA (1 μ g) intracutaneous injection. Following DNCB application, the levels of IL-4 and IFN-γ were determined using ELISA kits. (A) Levels of IL-4 in skin tissue were significantly increased. This increase in IL-4 was significantly attenuated in the mice receiving C5aRA intracutaneous injection. (B) Levels of IFN-γ in skin tissue was significantly increased. The increases in IFN-γ levels were significantly attenuated in the mice receiving C5aRA intracutaneous injection. Values are expressed as the mean ± standard deviation (n=5). ** P

    Article Snippet: Production of IL-4 and IFN-γ in skin tissue and IL-4, IFN-γ, histamine and IgE levels in serum were determined using ELISA kits (Mouse IL-4, Mouse IFN-γ, Porcine Histamine HIS and Mouse IgE ELISA kits; Sigma-Aldrich) according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Anti-CEACAM1 mAb treatment enhances CD8 + T cell expansion and viral control. a Experimental setup. b , c . One group of wild-type (WT) and Ceacam1 –/– mice received anti-CEACAM1 mAb, and another group received an equal amount of isotype antibody on day –1 and day 3, followed by infection with 2 × 10 4 PFU of LCMV-Docile on day 0. b , c Graphs showing percentage of virus-specific Tet-GP33 + CD8 + T cells in blood ( b ) and viral titers in serum ( c ) in indicated groups over the indicated times. Statistical significance is shown between the WT + IgG-treated groups and the WT + α-CC1 mAb-treated groups ( n = 3–6 per group). d Experimental setup. e Wild-type (WT) mice were infected with 2 × 10 6 PFU of LCMV-Docile on day 0. On day 16 after infection, mice were treated with 200 µg anti-CEACAM1 mAb per mouse or with an isotype antibody. e Viral titers in serum (left panel) over the indicated times and in indicated organs (right panel) on day 60 after infection ( n = 4–8 per group). f Experimental setup. g – i Ceacam1 –/– mice were given 1 × 10 4 P14 × CD45.1 WT splenocytes on day –2. One group of these mice was additionally treated with anti-CEACAM1 mAb, and another group was treated with an equal amount of an isotype antibody on day –1 and on day 3, followed by infection with 2 × 10 4 PFU of LCMV-Docile on day 0. Mice were analyzed on day 8 after infection. g Absolute number of transferred Tet-GP33 + CD45.1 + CD8 + T cells in blood and spleen of host Ceacam1 –/– (CD45.2) mice ( n = 5–7 per group). h Intracellular cytokine IFN-γ secretion from transferred P14 × CD45.1 WT CD8 + T cells ( n = 5–7 per group). i Viral titers in the indicated organs of the recipient mice ( n = 5–7 per group). * P

    Journal: Nature Communications

    Article Title: CEACAM1 promotes CD8+ T cell responses and improves control of a chronic viral infection

    doi: 10.1038/s41467-018-04832-2

    Figure Lengend Snippet: Anti-CEACAM1 mAb treatment enhances CD8 + T cell expansion and viral control. a Experimental setup. b , c . One group of wild-type (WT) and Ceacam1 –/– mice received anti-CEACAM1 mAb, and another group received an equal amount of isotype antibody on day –1 and day 3, followed by infection with 2 × 10 4 PFU of LCMV-Docile on day 0. b , c Graphs showing percentage of virus-specific Tet-GP33 + CD8 + T cells in blood ( b ) and viral titers in serum ( c ) in indicated groups over the indicated times. Statistical significance is shown between the WT + IgG-treated groups and the WT + α-CC1 mAb-treated groups ( n = 3–6 per group). d Experimental setup. e Wild-type (WT) mice were infected with 2 × 10 6 PFU of LCMV-Docile on day 0. On day 16 after infection, mice were treated with 200 µg anti-CEACAM1 mAb per mouse or with an isotype antibody. e Viral titers in serum (left panel) over the indicated times and in indicated organs (right panel) on day 60 after infection ( n = 4–8 per group). f Experimental setup. g – i Ceacam1 –/– mice were given 1 × 10 4 P14 × CD45.1 WT splenocytes on day –2. One group of these mice was additionally treated with anti-CEACAM1 mAb, and another group was treated with an equal amount of an isotype antibody on day –1 and on day 3, followed by infection with 2 × 10 4 PFU of LCMV-Docile on day 0. Mice were analyzed on day 8 after infection. g Absolute number of transferred Tet-GP33 + CD45.1 + CD8 + T cells in blood and spleen of host Ceacam1 –/– (CD45.2) mice ( n = 5–7 per group). h Intracellular cytokine IFN-γ secretion from transferred P14 × CD45.1 WT CD8 + T cells ( n = 5–7 per group). i Viral titers in the indicated organs of the recipient mice ( n = 5–7 per group). * P

    Article Snippet: For measurement of intracellular IFN-γ and TNF, cells were fixed with formaldehyde (2% formaldehyde solution in PBS) for 10 min, permeabilized with saponin (1%) solution, and stained with anti-IFN-γ (XMG 1.1) or TNF (MP6-XT22) antibodies (both from eBioscience).

    Techniques: Mouse Assay, Infection

    CEACAM1 is expressed on CD8 + T cells and is essential for resolving LCMV infection. a CEACAM1 expression on naïve CD8 + T cells (left panel) and Tet-GP33 + CD8 + T cells (right panel) from wild-type (WT; black line) or Ceacam1 –/– (red line) mice infected with 2 × 10 4 plaque-forming units (PFU) of LCMV-Docile at indicated time points. Isotype control antibody staining of T cells from WT mice is shown as a gray area ( n = 4–6 per group). b Absolute number of Tet-GP33 + and NP396 + CD8 + T cells in blood from WT or Ceacam1 –/– mice after intravenous infection with 200 PFU of LCMV-Docile at indicated time points ( n = 4–6 per group). c Absolute number of CD8 + T cells and percentages of Tet-GP33 + CD8 + T cells in spleen, lymph node, liver, lung, kidney, and blood ( n = 6–8 per group) from WT or Ceacam1 –/– mice on day 8 after infection with 200 or 2 × 10 4 PFU of LCMV-Docile. d , e Intracellular cytokine interferon-γ (IFN-γ) secretion in Tet-GP33 + CD8 + T cells ( d ) and median fluorescence intensity (MFI) levels in IFN-γ + Tet-GP33 + CD8 + T cells ( e ) from splenocytes of WT and Ceacam1 –/– mice on day 8 after infection with 200 or 2 × 10 4 PFU of LCMV-Docile. Horizontal dotted lines designate the detection limit without restimulation ( n = 6–8 per group). f Ratio of the percentage of IFN-γ + cells to the percentage of Tet-GP33 + CD8 + T cells from WT and Ceacam1 –/– mice on day 8 after infection with 200 ( n = 12) or 2 × 10 4 ( n = 7) PFU of LCMV-Docile. g Viral titers from WT and Ceacam1 –/– mice in indicated organs and serum 8 days after intravenous infection with 200 or 2 × 10 4 PFU of LVMC-Docile ( n = 6–8 per group). * P

    Journal: Nature Communications

    Article Title: CEACAM1 promotes CD8+ T cell responses and improves control of a chronic viral infection

    doi: 10.1038/s41467-018-04832-2

    Figure Lengend Snippet: CEACAM1 is expressed on CD8 + T cells and is essential for resolving LCMV infection. a CEACAM1 expression on naïve CD8 + T cells (left panel) and Tet-GP33 + CD8 + T cells (right panel) from wild-type (WT; black line) or Ceacam1 –/– (red line) mice infected with 2 × 10 4 plaque-forming units (PFU) of LCMV-Docile at indicated time points. Isotype control antibody staining of T cells from WT mice is shown as a gray area ( n = 4–6 per group). b Absolute number of Tet-GP33 + and NP396 + CD8 + T cells in blood from WT or Ceacam1 –/– mice after intravenous infection with 200 PFU of LCMV-Docile at indicated time points ( n = 4–6 per group). c Absolute number of CD8 + T cells and percentages of Tet-GP33 + CD8 + T cells in spleen, lymph node, liver, lung, kidney, and blood ( n = 6–8 per group) from WT or Ceacam1 –/– mice on day 8 after infection with 200 or 2 × 10 4 PFU of LCMV-Docile. d , e Intracellular cytokine interferon-γ (IFN-γ) secretion in Tet-GP33 + CD8 + T cells ( d ) and median fluorescence intensity (MFI) levels in IFN-γ + Tet-GP33 + CD8 + T cells ( e ) from splenocytes of WT and Ceacam1 –/– mice on day 8 after infection with 200 or 2 × 10 4 PFU of LCMV-Docile. Horizontal dotted lines designate the detection limit without restimulation ( n = 6–8 per group). f Ratio of the percentage of IFN-γ + cells to the percentage of Tet-GP33 + CD8 + T cells from WT and Ceacam1 –/– mice on day 8 after infection with 200 ( n = 12) or 2 × 10 4 ( n = 7) PFU of LCMV-Docile. g Viral titers from WT and Ceacam1 –/– mice in indicated organs and serum 8 days after intravenous infection with 200 or 2 × 10 4 PFU of LVMC-Docile ( n = 6–8 per group). * P

    Article Snippet: For measurement of intracellular IFN-γ and TNF, cells were fixed with formaldehyde (2% formaldehyde solution in PBS) for 10 min, permeabilized with saponin (1%) solution, and stained with anti-IFN-γ (XMG 1.1) or TNF (MP6-XT22) antibodies (both from eBioscience).

    Techniques: Infection, Expressing, Mouse Assay, Staining, Fluorescence

    The action of CEACAM1 is intrinsic to T cells. Representative flow cytometry histogram of proliferating CD8 + T cells ( a ) and bar diagram showing percentage of proliferated CD8 + T cells ( b ) from P14 × wild-type (WT) (black line) or P14 × Ceacam1 –/– (red line) mice that were adoptively transferred into WT (CD45.1) mice (1 × 10 7 cells per mouse) on day −1. Mice were infected with 200 PFU of LCMV-Docile on day 0, and splenic tissue samples were examined 4 days after infection. c Experimental setup. d – f We transferred 1 × 10 4 P14 × WT or P14 × Ceacam1 –/– (CD45.2) splenocytes into naïve CD45.1 WT mice on day –1 and then infected them with 2 × 10 4 PFU of LCMV-Docile on day 0, analyzed on day 8 after infection. d Percentage of Tet-GP33 + CD45.2 + CD8 + T cells in blood ( n = 6–7) and in spleen ( n = 10). e Intracellular cytokine IFN-γ secretion by splenocytes from P14 × WT or P14 × Ceacam1 –/– mice. Horizontal dotted lines designate the detection limit without restimulation ( n = 6–7 per group). f Viral titers in indicated organs from CD45.1 WT mice ( n = 3–4 per group). g–i Statistical analysis of CD8 + T cell population or Tet-GP33 + CD8 + T cells from murine bone marrow chimeras reconstituted with 1:1 composition of bone marrow from CD45.1 WT:CD45.2 WT mice in one group and bone marrow from CD45.1 WT: Ceacam1 –/– (CD45.2) mice in another group after 45 days of reconstitution before infection, as measured by flow cytometry in blood ( g , n = 8 per group), and in blood, spleen, and lymph nodes 8 days after infection with 200 PFU ( h ) or 2 × 10 4 PFU ( i ) of LCMV-Docile ( n = 5 per group). * P

    Journal: Nature Communications

    Article Title: CEACAM1 promotes CD8+ T cell responses and improves control of a chronic viral infection

    doi: 10.1038/s41467-018-04832-2

    Figure Lengend Snippet: The action of CEACAM1 is intrinsic to T cells. Representative flow cytometry histogram of proliferating CD8 + T cells ( a ) and bar diagram showing percentage of proliferated CD8 + T cells ( b ) from P14 × wild-type (WT) (black line) or P14 × Ceacam1 –/– (red line) mice that were adoptively transferred into WT (CD45.1) mice (1 × 10 7 cells per mouse) on day −1. Mice were infected with 200 PFU of LCMV-Docile on day 0, and splenic tissue samples were examined 4 days after infection. c Experimental setup. d – f We transferred 1 × 10 4 P14 × WT or P14 × Ceacam1 –/– (CD45.2) splenocytes into naïve CD45.1 WT mice on day –1 and then infected them with 2 × 10 4 PFU of LCMV-Docile on day 0, analyzed on day 8 after infection. d Percentage of Tet-GP33 + CD45.2 + CD8 + T cells in blood ( n = 6–7) and in spleen ( n = 10). e Intracellular cytokine IFN-γ secretion by splenocytes from P14 × WT or P14 × Ceacam1 –/– mice. Horizontal dotted lines designate the detection limit without restimulation ( n = 6–7 per group). f Viral titers in indicated organs from CD45.1 WT mice ( n = 3–4 per group). g–i Statistical analysis of CD8 + T cell population or Tet-GP33 + CD8 + T cells from murine bone marrow chimeras reconstituted with 1:1 composition of bone marrow from CD45.1 WT:CD45.2 WT mice in one group and bone marrow from CD45.1 WT: Ceacam1 –/– (CD45.2) mice in another group after 45 days of reconstitution before infection, as measured by flow cytometry in blood ( g , n = 8 per group), and in blood, spleen, and lymph nodes 8 days after infection with 200 PFU ( h ) or 2 × 10 4 PFU ( i ) of LCMV-Docile ( n = 5 per group). * P

    Article Snippet: For measurement of intracellular IFN-γ and TNF, cells were fixed with formaldehyde (2% formaldehyde solution in PBS) for 10 min, permeabilized with saponin (1%) solution, and stained with anti-IFN-γ (XMG 1.1) or TNF (MP6-XT22) antibodies (both from eBioscience).

    Techniques: Flow Cytometry, Cytometry, Mouse Assay, Infection