Journal: Nature communications

Article Title: VSV ∆M51 drives CD8 + T cell-mediated tumour regression through infection of both cancer and non-cancer cells.

doi: 10.1038/s41467-024-54111-6

Figure Lengend Snippet: Fig. 8 | Type I IFN and TNF induce costimulatory marker expression on DCs in human tumours. a Schematic of experiment using human head and neck tumour samples. b Frequency of total DCs (left) and CD141+ DCs (right) in human tumors treated for 24 h ex vivo with media or hTNF/hIFNβ (20 ng/mL hTNF and 50 ng/mL hIFNβ) (n = 4 patient tumours per condition, compiled from 4 independent experiments, two-tailed, paired t test). c–e Expression of HLA-ABC (c), CD80 (d)

Article Snippet: The next day, cells were infected with 1, 5 or 10MOI of VSVΔM51-creGFP in serum free RPMI and incubated for 1 h at 37 °C, followed by removal of VSVΔM51-creGFP-containing media and replacement with complete RPMI containing 2.5 ng/mL recombinant mouse IFNβ (R&D systems, 8234-MB-010).

Techniques: Marker, Expressing, Ex Vivo, Two Tailed Test