Journal: Oncotarget

Article Title: Assessment of bevacizumab resistance increased by expression of BCAT1 in IDH1 wild-type glioblastoma: application of DSC perfusion MR imaging

doi: 10.18632/oncotarget.11901

Figure Lengend Snippet: Immunohistochemistry images showing differential expression of BCAT1, KI-67, CD34, and HIF-1α from the IDH1 WT and BCAT1 sh#1 tumors. The first row shows a decreased BCAT1 level in BCAT1 sh#1 tissue. The rate of cell proliferation evaluated by KI-67 (second row), vascularity by CD34 (third row), and HIF-1a expression level (forth row) are decreased in BCAT1 sh#1 tumors. The expression is evaluated by DAB and shows the original magnification of ×40.

Article Snippet: Sections were then incubated with the primary mouse monoclonal antibody to BCAT1 (BD Biosciences), mouse monoclonal antibody to human IDH1 R132H (Dianova), mouse monoclonal antibody to human KI67 (UM800033, ORIGENE), goat polyclonal antibody to rat CD34 (R&D Systems), or mouse monoclonal antibody to human HIF-1 alpha (Thermo) in Dako REAL antibody diluent (Dako).

Techniques: Immunohistochemistry, Quantitative Proteomics, Expressing

Journal: Cell Transplantation

Article Title: Adipose-derived stem cells inhibit dendritic cell migration by secreting tumor necrosis factor-α-stimulated gene 6 to improve the allogeneic skin transplantation survival rate in mice

doi: 10.1177/09636897251376125

Figure Lengend Snippet: Characteristics of adipose-derived stem cells. (a) Positive expression for CD90 and CD105 and negative expression for CD19 and CD34. (b), (c) Differentiation of ADSCs into adipocytes and osteoblasts. After adipogenic differentiation, newly differentiated adipocytes had lipid droplets identified by Oil Red O staining. Osteogenic differentiation was confirmed by Alizarin Red S staining. ADSCs: adipose-derived stem cells; CD: cluster of differentiation.

Article Snippet: Passage 3 cells were stained with antibodies against CD90 (E-AB-F1283C) (Elabscience, Wuhan, China), CD105 (E-AB-F1233UE) (Elabscience, Wuhan, China), CD19 (E-AB-F1004E) (Elabscience, Wuhan, China), and CD34 (E-AB-F1284D) (Elabscience, Wuhan, China).

Techniques: Derivative Assay, Expressing, Staining

Journal: Cell Transplantation

Article Title: Adipose-derived stem cells inhibit dendritic cell migration by secreting tumor necrosis factor-α-stimulated gene 6 to improve the allogeneic skin transplantation survival rate in mice

doi: 10.1177/09636897251376125

Figure Lengend Snippet: Adipose-derived stem cells inhibited dendritic cell migration in vivo . (a) Mouse skin allotransplantation and phosphate-buffered saline (PBS) or cell infusion. (b) Expression of dendritic cells (DCs) in grafted skin. Data are expressed as fold-changes relative to levels in the control group. Statistically significant differences among groups are reported above the columns (*** P < 0.001). (c) Immunofluorescence findings. CD80 (red) and CD86 (green) are commonly used as markers for dendritic cells. The expression levels of DCs in the control group were lower than in the adipose-derived stem cell (ADSC) group. (d) Comparison of histologic changes among the two groups. Inflammatory reactions were more prominent in the control group than in the experimental group on postoperative Day 7 (×4 or ×10). CD: cluster of differentiation.

Article Snippet: Passage 3 cells were stained with antibodies against CD90 (E-AB-F1283C) (Elabscience, Wuhan, China), CD105 (E-AB-F1233UE) (Elabscience, Wuhan, China), CD19 (E-AB-F1004E) (Elabscience, Wuhan, China), and CD34 (E-AB-F1284D) (Elabscience, Wuhan, China).

Techniques: Derivative Assay, Migration, In Vivo, Saline, Expressing, Control, Immunofluorescence, Comparison

Journal: Cell Transplantation

Article Title: Adipose-derived stem cells inhibit dendritic cell migration by secreting tumor necrosis factor-α-stimulated gene 6 to improve the allogeneic skin transplantation survival rate in mice

doi: 10.1177/09636897251376125

Figure Lengend Snippet: Tumor necrosis factor-α-stimulated gene 6 played a key role in inhibiting dendritic cell maturation and migration. (a) Flow cytometry analysis of dendritic cell (DC) maturation. DCs were co-cultured with adipose-derived stem cells (ADSCs) or individual immunomodulatory factors (TSG-6, HGF, TGFβ, Galectin-1), followed by staining for CD80 and CD86. Quantification of mature DCs is shown on the right. (*** P < 0.001 vs control). (b) Transwell migration assay to assess DC motility after co-culture with ADSCs or indicated factors. Representative images (left) and quantification of migrated cells (right) (*** P < 0.001). (c) Cell Counting Kit-8 (CCK-8) assay for DC viability. CD: cluster of differentiation; TSG-6: tumor necrosis factor-α-stimulated gene 6; HGF: hepatocyte growth factor; TGFβ: transforming growth factor beta.

Article Snippet: Passage 3 cells were stained with antibodies against CD90 (E-AB-F1283C) (Elabscience, Wuhan, China), CD105 (E-AB-F1233UE) (Elabscience, Wuhan, China), CD19 (E-AB-F1004E) (Elabscience, Wuhan, China), and CD34 (E-AB-F1284D) (Elabscience, Wuhan, China).

Techniques: Migration, Flow Cytometry, Cell Culture, Derivative Assay, Staining, Control, Transwell Migration Assay, Co-Culture Assay, Cell Counting, CCK-8 Assay

Journal: Cell Transplantation

Article Title: Adipose-derived stem cells inhibit dendritic cell migration by secreting tumor necrosis factor-α-stimulated gene 6 to improve the allogeneic skin transplantation survival rate in mice

doi: 10.1177/09636897251376125

Figure Lengend Snippet: Tumor necrosis factor-α-stimulated gene 6 secreted by adipose-derived stem cells inhibited dendritic cell migration in vitro . (a, b) Tumor necrosis factor-α-stimulated gene 6 (TSG-6) effectively inhibited the migration of dendritic cells (DCs), and this inhibitory effect became more pronounced with increasing TSG-6 concentrations. (c, d) The ability of transfected adipose-derived stem cells (ADSCs) to inhibit the migration of DCs was weakened. When TSG-6 was added again, the ability of transfected ADSCs to inhibit the migration of DCs was restored (* P < 0.05; ** P < 0.01).

Article Snippet: Passage 3 cells were stained with antibodies against CD90 (E-AB-F1283C) (Elabscience, Wuhan, China), CD105 (E-AB-F1233UE) (Elabscience, Wuhan, China), CD19 (E-AB-F1004E) (Elabscience, Wuhan, China), and CD34 (E-AB-F1284D) (Elabscience, Wuhan, China).

Techniques: Derivative Assay, Migration, In Vitro, Transfection

Journal: Cell Transplantation

Article Title: Adipose-derived stem cells inhibit dendritic cell migration by secreting tumor necrosis factor-α-stimulated gene 6 to improve the allogeneic skin transplantation survival rate in mice

doi: 10.1177/09636897251376125

Figure Lengend Snippet: Transcriptomic analysis and functional enrichment of dendritic cells under different treatments. (a) Volcano plots of differentially expressed genes (DEGs) comparing ADSCs versus control (top) and TSG-6 versus control (bottom) groups. Red and blue dots indicate upregulated and downregulated genes, respectively (|log2FC| > 1, P < 0.05). (b) RT-qPCR validation of selected DEGs involved in immune modulation (ADM, GHRH, NDRG1, SELENBP1, etc.) in DCs from control, TSG-6, and ADSC groups (* P < 0.05). (c) GO enrichment analysis of DEGs in the ADSC (left) and TSG-6 (right) groups, showing the top 30 terms from biological processes (BP), molecular functions (MF), and cellular components (CC). (d) KEGG pathway enrichment of DEGs in ADSC (left) and TSG-6 (right) groups. Bubble size indicates gene count, and color represents adjusted p -values (C: control group; A: ADSC group; T: TSG-6 group). ADSC: adipose-derived stem cell; TSG-6: tumor necrosis factor-α-stimulated gene 6; RT-qPCR: real-time quantitative polymerase chain reaction; ADM: adrenomedullin; GHRH: growth hormone–releasing hormone; SELENBP1: selenium binding protein 1; NDRG1: N-myc downstream regulated gene-1; GO: gene ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes.

Article Snippet: Passage 3 cells were stained with antibodies against CD90 (E-AB-F1283C) (Elabscience, Wuhan, China), CD105 (E-AB-F1233UE) (Elabscience, Wuhan, China), CD19 (E-AB-F1004E) (Elabscience, Wuhan, China), and CD34 (E-AB-F1284D) (Elabscience, Wuhan, China).

Techniques: Functional Assay, Control, Quantitative RT-PCR, Biomarker Discovery, Derivative Assay, Real-time Polymerase Chain Reaction, Binding Assay