Journal: Stem Cell Research & Therapy

Article Title: Single cell and lineage tracing studies reveal the impact of CD34 + cells on myocardial fibrosis during heart failure

doi: 10.1186/s13287-023-03256-0

Figure Lengend Snippet: Effect of depletion of CD34 + cells in myocardial fibrosis. A Schematic showing CD34-CreERT2;Rosa26-tdTomato/DTA mice. Experimental scheme whereby Cre/DTA mice were given tamoxifen for 1 weeks before TAC 0 or TAC surgery. Heart tissue was harvested 4 weeks after TAC surgery. B Representative echocardiography of mice between CD34-CreERT2;Rosa26-tdTomato (Control) and CD34-CreERT2;Rosa26-tdTomato/DTA (Cre/DTA) at 4 weeks after TAC surgery. C Echocardiographic measurement of left ventricle ejection fraction (EF) and fractional shortening (FS) of control group and Cre/DTA group after 4 weeks TAC surgery, Data represent mean ± SEM, Cre/DTA group, n = 17; Control group, n = 23. D ELISA detection of the serum ANP and BNP between control group and Cre/DTA group after 4 weeks TAC surgery, Data represent mean ± SEM, n = 15 per group. E Representative immunostaining images showing staining of tdTomato and vimentin, DDR2 in control group and Cre/DTA group (Scale bar = 20 μm and 100 μm). n = 10 per group. F Graph showing percentage of tdTomato expression in vimentin + , DDR2 + fibroblast. Data represent mean ± SEM, n = 10

Article Snippet: The levels of ANP and BNP in mouse serum were detected by Mouse ANP ELISA kit (Abcam, ab267800) and Mouse BNP ELISA kit (Novus, NBP2-70,011) according to the protocol provided.

Techniques: Control, Enzyme-linked Immunosorbent Assay, Immunostaining, Staining, Expressing

Journal: Journal of Inflammation Research

Article Title: AST-120 Improves Cardiac Dysfunction in Acute Kidney Injury Mice via Suppression of Apoptosis and Proinflammatory NF-κB/ICAM-1 Signaling

doi: 10.2147/JIR.S283378

Figure Lengend Snippet: AST-120 administration decreased cardiac dysfunction following renal I/R injury. ( A ) Echocardiographic images were taken before surgery (pre-op) and 48 h after renal I/R injury (post-op). Representative M-mode echocardiographic tracings (24 min) are presented. ( B – D ) Data from echocardiograms. n = 6 per group. ( E ) BNP levels in the plasma from the sham, AKI-V, and AKI-AST groups measured by ELISA. n = 5 per group; the data are expressed as mean ± SEM, * p < 0.05 vs the pre-op group; # p < 0.05 vs the AKI-V group.

Article Snippet: Human and mouse IL-1β, and mouse brain natriuretic peptide (BNP), were determined using commercially available enzyme-linked immunosorbent assay (ELISA) kits (RayBiotech, GA, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Cardiovascular Diabetology

Article Title: β Cell-specific deletion of guanylyl cyclase A, the receptor for atrial natriuretic peptide, accelerates obesity-induced glucose intolerance in mice

doi: 10.1186/s12933-018-0747-3

Figure Lengend Snippet: The insulinotropic effects of exogenous BNP are abolished in β GC-A KO mice. Fasted and oral glucose-stimulated plasma insulin levels (GSIS, top) as well as oral glucose tolerance (oGTT, bottom) were determined in 16-h-fasted control ( a ) and β GC-A KO littermates ( b ) after 7 days of subcutaneous vehicle or BNP infusions via osmotic minipumps (n = 6 mice per genotype). *P < 0.05 and **P < 0.01 vs basal; # P < 0.05 vs vehicle treatment (GSIS) or baseline (oGTT before BNP)

Article Snippet: Mouse BNP (Bachem) was infused via subcutaneously implanted osmotic minipumps (model 1007D; Alzet Corporation) at a dose of 2 ng/h/g BW over 7 days [ ].

Techniques: Clinical Proteomics, Control