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MMAB mouse monoclonal antibody clone OTI5G1 formerly 5G1 HRP conjugated
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Image Search Results
Journal: bioRxiv
Article Title: Synaptotagmin 2 is ectopically overexpressed in excitatory presynapses of a widely used CaMKIIα-Cre mouse line
doi: 10.1101/2021.06.30.450492
Figure Lengend Snippet: ( A ) qPCR analysis of mRNA expression in the hippocampus in indicated mouse lines. Syt2-Int8 represents premRNA of Syt2 . Values were normalized by Gapdh expression level and are shown as fold increases against Cre-controls. Pgk1 is another housekeeping gene. ( B, C ) Western blotting images of different brain regions. CB, cerebellum; CC, cerebral cortex; HP, hippocampus; MB, midbrain; MO, medula oblongata; OB, olfactory bulb. ( D ) Quantification results of C. Values were normalized against α-tubulin expression level and are shown as fold increases against Cre-controls. ( E ) Western blotting images of hippocampal lysates showing Syt2 expression levels in various Cre-expressing methodologies. Viral concentration was diluted 2x in three steps for AAV-hSyn-Cre expression. *, nonspecific band. Mean ± SD is shown. **P < 0.01; ***P < 0.001. N = 4 (biological replicates). Statistical tests were performed using two-tailed, unpaired t-tests.
Article Snippet: Antibodies used were rabbit polyclonal antibody (RpAb) to Syt1 (1:1000) from Synaptic Systems (105103), mouse monoclonal antibody (MmAb) to
Techniques: Expressing, Western Blot, Concentration Assay, Two Tailed Test
Journal: bioRxiv
Article Title: Synaptotagmin 2 is ectopically overexpressed in excitatory presynapses of a widely used CaMKIIα-Cre mouse line
doi: 10.1101/2021.06.30.450492
Figure Lengend Snippet: ( A ) Immunofluorescent images of Syt2 staining in hippocampi of CamiCre mice. ( B ) Super-resolution images of the hippocampal CA3 region corresponding to the approximate areas indicated by the white dotted boxes in A are shown. sp, stratum pyramidale; sr, stratum radiatum. ( C ) Colocalization was analyzed using the Confined Displacement Algorithm on the neuropil area of CA1 and CA3. R, Pearson correlation coefficient; M1 and M2, Manders coefficient. NS, Not significant; ***P < 0.001 (the significance of correlation or colocalization were calculated by comparing original images to random displacement images). Scale bars: (A) 250 μm; (B) 10 μm.
Article Snippet: Antibodies used were rabbit polyclonal antibody (RpAb) to Syt1 (1:1000) from Synaptic Systems (105103), mouse monoclonal antibody (MmAb) to
Techniques: Staining