Journal: Drug Design, Development and Therapy

Article Title: Astragaloside IV improves renal function and fibrosis via inhibition of miR-21-induced podocyte dedifferentiation and mesangial cell activation in diabetic mice

doi: 10.2147/dddt.s170840

Figure Lengend Snippet: Figure 3 Effect of AS-IV on miR-21 overexpression-induced podocyte dedifferentiation and MC activation. (A–D) Real-time PCR and Western blot results showed that miR-21 overexpression decreased nephrin expression and increased α-SMA expression in podocyte. AS-IV treatment reversed this effect. (E and F) Real-time PCR and Western blot results showed that AS-IV treatment decreased the levels of α-SMA in miR-21-overexpression-stimulated MCs. Data are presented as mean ± SD. n=3. *Compared with NC, P,0.05; #compared with miR-21 mimics group, P,0.05. Abbreviations: α-SMA, α-smooth muscle actin; AS-IV, astragaloside IV; DAPI, 4′,6-diamidino-2-phenylindole; DKD, diabetic kidney disease; HG, high glucose; MC, mesangial cell; miR-21, microRNA-21; NC, normal control.

Article Snippet: Rabbit monoclonal anti-nephrin antibody was provided by Novus Biologicals (Littleton, CO, USA). miR-21 mimics and negative controls were synthesized by Ribobio (Guangzhou, China). cell culture As previously described, primary podocytes were obtained from male C57BL/6J mice (30–40 g).18 Under brief diethyl ether anesthesia, mice kidneys were excised.

Techniques: Over Expression, Activation Assay, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Control

Journal: Drug Design, Development and Therapy

Article Title: Astragaloside IV improves renal function and fibrosis via inhibition of miR-21-induced podocyte dedifferentiation and mesangial cell activation in diabetic mice

doi: 10.2147/dddt.s170840

Figure Lengend Snippet: Figure 4 Effect of AS-IV on the expression of nephrin and α-SMA in vivo. Immunofluorescence images showed that AS-IV increased the expression of nephrin and decreased the expression of α-SMA in glomerulus (white arrows) compared with the untreated DKD mice. Abbreviations: α-SMA, α-smooth muscle actin; AS-IV, astragaloside IV; DKD, diabetic kidney disease.

Article Snippet: Rabbit monoclonal anti-nephrin antibody was provided by Novus Biologicals (Littleton, CO, USA). miR-21 mimics and negative controls were synthesized by Ribobio (Guangzhou, China). cell culture As previously described, primary podocytes were obtained from male C57BL/6J mice (30–40 g).18 Under brief diethyl ether anesthesia, mice kidneys were excised.

Techniques: Expressing, In Vivo, Immunofluorescence

Journal: Drug Design, Development and Therapy

Article Title: Astragaloside IV improves renal function and fibrosis via inhibition of miR-21-induced podocyte dedifferentiation and mesangial cell activation in diabetic mice

doi: 10.2147/dddt.s170840

Figure Lengend Snippet: Figure 7 Effect of the Wnt/β-catenin pathway and the TGF-β1/Smads pathway on podocyte dedifferentiation and MC activation. (A–C) Western blot results showed that both SB431542 and XAV-939 decreased nephrin expression in podocytes and increased the expression of α-SMA in podocytes and MCs co-treated with miR-21 and AS-IV. Data are presented as mean ± SD. n=3. *Compared with co-treatment with miR-21 mimics and AS-IV group, P,0.05. Abbreviations: α-SMA, α-smooth muscle actin; AS-IV, astragaloside IV; MC, mesangial cell; miR-21, microRNA-21; TGF, transforming growth factor.

Article Snippet: Rabbit monoclonal anti-nephrin antibody was provided by Novus Biologicals (Littleton, CO, USA). miR-21 mimics and negative controls were synthesized by Ribobio (Guangzhou, China). cell culture As previously described, primary podocytes were obtained from male C57BL/6J mice (30–40 g).18 Under brief diethyl ether anesthesia, mice kidneys were excised.

Techniques: Activation Assay, Western Blot, Expressing