Journal: Aging Cell

Article Title: Extra virgin olive oil improves synaptic activity, short‐term plasticity, memory, and neuropathology in a tauopathy model

doi: 10.1111/acel.13076

Figure Lengend Snippet: Effect of chronic administration of EVOO‐rich diet on hippocampal pre‐ and postsynaptic protein expression in hTau mice. (a) Representative Western blot analyses for SNAP25, syntaxin 1A, synaptobrevin, complexin 1/2, and synaptophysin (SYP) in hippocampus homogenates from CTR and EVOO mice. (b) Densitometric analyses of the immunoreactivities to the antibodies shown in panel A (** p < .01; n = 6 per group) . (c) Representative Western blot analyses for the AMPAR subunit glutamate receptor 1 (GluR1), AMPAR subunit glutamate receptor 2 (GluR2), and their phosphorylated forms, pGluR1 (S831), pGluR1 (S845), and pGluR2 (S880), in hippocampus homogenates from CTR and EVOO mice. (d) Densitometric analyses of the immunoreactivities to the antibodies shown in panel A ( n = 6 per group) . Values are mean ± SEM

Article Snippet: Syntaxin 1A , AF7237 , RD SYSTEMS , Goat.

Techniques: Expressing, Western Blot

Journal: Aging Cell

Article Title: Extra virgin olive oil improves synaptic activity, short‐term plasticity, memory, and neuropathology in a tauopathy model

doi: 10.1111/acel.13076

Figure Lengend Snippet: Antibodies used in the study

Article Snippet: Syntaxin 1A , AF7237 , RD SYSTEMS , Goat.

Techniques:

Journal: Frontiers in immunology

Article Title: The E3 Ubiquitin Ligase TRIM65 Negatively Regulates Inflammasome Activation Through Promoting Ubiquitination of NLRP3.

doi: 10.3389/fimmu.2021.741839

Figure Lengend Snippet: FIGURE 2 | TRIM65 inhibits NLRP3 inflammasome activation in macrophage. (A) PMA-differentiated and LPS-primed THP-1 cells stably expressing shRNA targeting TRIM65 mRNA were stimulated with MSU, R837 or nigericin. Cleaved IL-1b, activated caspase-1 (P20) in the SN and pro-IL-1b and pro-caspase-1 in the input were analyzed by western blotting. (B-F) LPS-primed BMDMs from wild-type and Trim65-/- mice were stimulated with MSU, ATP, nigericin (B–E), or cytosolic LPS (F). Cleaved IL-1b, activated caspase-1 (P20) in the culture supernatants and pro-IL-1b and pro-caspase-1 in cell lysates were analyzed by western blotting (B). Cleaved IL-1b in the culture supernatants was assayed by ELISA (C–F). Data are representative of three independent experiments of duplicate biological repeats (A, B). SN, media supernatants; Input, cell extracts. Student’s t-test, ***P < 0.001, ****P < 0.0001. Data are shown as the means ± SEM of three independent experiments (C–F).

Article Snippet: Anti-mouse IL-1b antibody was obtained from R&D Systems (MPLS, USA).

Techniques: Activation Assay, Stable Transfection, Expressing, shRNA, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in immunology

Article Title: The E3 Ubiquitin Ligase TRIM65 Negatively Regulates Inflammasome Activation Through Promoting Ubiquitination of NLRP3.

doi: 10.3389/fimmu.2021.741839

Figure Lengend Snippet: FIGURE 5 | TRIM65 signaling prevents LPS-induced systemic inflammation and MSU-induced peritonitis and gouty arthritis via suppression of the NLRP3 inflammasome. (A) Wild-type or Trim65-/- mice (5 mice per group) were intraperitoneally injected with LPS (15 mg/kg) and treated for 4.5 h. The sera were collected, and the levels of IL-1b were measured by ELISA. (B, C) Wild-type or Trim65-/- mice (4-5 mice per group) were intraperitoneally injected with MSU crystals (1 mg/ mouse) and treated for 6h. The numbers of neutrophils in the peritoneal cavity were analyzed by FACS (B), and the levels of IL-1b (C) were assayed by ELISA. (D–F) Wild-type or Trim65-/- mice (7-10 mice per group) were intraperitoneally injected with MSU crystals, after which joint swelling (D) at different time points was measured with a Vernier caliper, the levels of IL-1b (E) in the supernatants of joint cultures at 24 h were assayed by ELISA, and changes in joint swelling at 24 h were compared (F). Data are shown as the means ± SEM of two or three independent experiments. Student’s t-test, ***P < 0.001.

Article Snippet: Anti-mouse IL-1b antibody was obtained from R&D Systems (MPLS, USA).

Techniques: Injection, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Rivastigmine Lowers Aβ and Increases sAPPα Levels, Which Parallel Elevated Synaptic Markers and Metabolic Activity in Degenerating Primary Rat Neurons

doi: 10.1371/journal.pone.0021954

Figure Lengend Snippet: Levels of low molecular weight neuronal sAPP (LMW-sAPP), high molecular weight glial sAPP (HMW-sAPP), and sAPPα were compared to levels of the presynaptic protein markers SNAP-25 and syntaxin-4, and the postsynaptic protein marker PSD-95. All values are expressed as a % of vehicle-treated cells for comparison. PSD-95 and SNAP-25 levels increased dose-dependently with rivastigmine treatment (both p<0.05), and syntaxin-4 levels increased but this change did not reach significance (7A and 7B). LMW-sAPP was increased with 5 µM rivastigmine but plateaued at the higher concentration. HMW-sAPP increased to a lesser extent at 5 µM, but was similar to vehicle at the higher concentration (7C). These data suggest that increased neuronal and decreased glial sAPP may be involved in the enhanced neuronal and synaptic marker stability that results from rivastigmine treatment, and that modulation of α-secretase may be involved in these effects.

Article Snippet: Blots of the lysates were probed with mouse-anti-SNAP-25 (Millipore), mouse-anti-PSD-95 (Antibodies Incorporated, Davis, CA), mouse-anti-syntaxin-4 (BD Transduction Labs, Franklin Lakes, NJ), mouse anti-β-actin (Sigma-Aldrich), and the 22C11 antibody as above, followed by the appropriate secondary antibody and ECL detection.

Techniques: Molecular Weight, Marker, Concentration Assay