Journal: Saudi Journal of Biological Sciences

Article Title: Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

doi: 10.1016/j.sjbs.2021.02.047

Figure Lengend Snippet: Histology of rats placental without treatments (C-l: A. Cytochrome c (50 µm), B. FasL (50 µm), C. Apoptosis (50 µm). D. Cytochrome c (16 µm), E. FasL (16 µm), F. Apoptosis (16 µm). EC: Epithelial Cells, FV: Fetal vessels.

Article Snippet: FasL detection used Fas-L mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 with PBS, Santa Crus Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection used a monoclonal mouse anti-cytochrome C antibody (ready to use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and ≤0.09% sodium aide (NaN 3 ).

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

doi: 10.1016/j.sjbs.2021.02.047

Figure Lengend Snippet: Histology of hypertension rats placental (C+): A. Cytochrome c (20 µm), B. FasL (20 µm), C. Apoptosis (20 µm). D. Cytochrome c (20 µm), E. FasL (20 µm), F. Apoptosis (20 µm). EC: Epithelial Cells, FV: Fetal vessels.

Article Snippet: FasL detection used Fas-L mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 with PBS, Santa Crus Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection used a monoclonal mouse anti-cytochrome C antibody (ready to use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and ≤0.09% sodium aide (NaN 3 ).

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

doi: 10.1016/j.sjbs.2021.02.047

Figure Lengend Snippet: Histology of hypertension rats placental given Extra virgin olive oil (EVOO) (T1) A. Cytochrome c (20 µm), B. FasL (20 µm), C. Apoptosis (20 µm). D. Cytochrome c (20 µm), E. FasL (20 µm), F. Apoptosis (20 µm). EC: Epithelial Cells, FV: Fetal vessels. SA: Spiral artery.

Article Snippet: FasL detection used Fas-L mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 with PBS, Santa Crus Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection used a monoclonal mouse anti-cytochrome C antibody (ready to use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and ≤0.09% sodium aide (NaN 3 ).

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

doi: 10.1016/j.sjbs.2021.02.047

Figure Lengend Snippet: Histology of hypertension rats placental given nanoherbal ZA (T2) A. Cytochrome c (20 µm), B. FasL (20 µm), C. Apoptosis (20 µm). D. Cytochrome c (20 µm), E. FasL (20 µm), F. Apoptosis (20 µm). EC: Epithelial Cells, FV: Fetal vessels.

Article Snippet: FasL detection used Fas-L mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 with PBS, Santa Crus Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection used a monoclonal mouse anti-cytochrome C antibody (ready to use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and ≤0.09% sodium aide (NaN 3 ).

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

doi: 10.1016/j.sjbs.2021.02.047

Figure Lengend Snippet: Histology of hypertension rats placental given EVOO and nanoherbal ZA (T3). A. Cytochrome c (20 µm), B. FasL (20 µm), C. Apoptosis (20 µm). D. Cytochrome c (20 µm), E. FasL (20 µm), F. Apoptosis (20 µm). EC: Epithelial Cells, FV: Fetal vessels.

Article Snippet: FasL detection used Fas-L mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 with PBS, Santa Crus Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection used a monoclonal mouse anti-cytochrome C antibody (ready to use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and ≤0.09% sodium aide (NaN 3 ).

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

doi: 10.1016/j.sjbs.2021.02.047

Figure Lengend Snippet: The Positive index of cytochrome c expression on placental histology.

Article Snippet: FasL detection used Fas-L mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 with PBS, Santa Crus Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection used a monoclonal mouse anti-cytochrome C antibody (ready to use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and ≤0.09% sodium aide (NaN 3 ).

Techniques: Expressing

Journal: Molecules

Article Title: Vernonia amygdalina Ethanol Extract Protects against Doxorubicin-Induced Cardiotoxicity via TGFβ, Cytochrome c, and Apoptosis

doi: 10.3390/molecules28114305

Figure Lengend Snippet: The positive index of cytochrome c expression on cardiac tissue.

Article Snippet: TGFβ detection utilized TGFβ mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 in PBS, Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bcl-2 detection utilized Bcl-2 rat monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 in PBS, Santa Cruz Biotechnology, Santa Cruz, CA, USA), collagen IV detection utilized collagen IV mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 in PBS, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection utilized a monoclonal mouse anti-cytochrome C antibody (ready-to-use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and 0.09% (NaN3).

Techniques: Expressing

Journal: Molecules

Article Title: Vernonia amygdalina Ethanol Extract Protects against Doxorubicin-Induced Cardiotoxicity via TGFβ, Cytochrome c, and Apoptosis

doi: 10.3390/molecules28114305

Figure Lengend Snippet: Histology of cardiac-tissue-stained cytochrome c (K-: rats induced with doxorubicin 15 mg/kgbw, K+: rats induced with doxorubicin 15 mg/kgbw + quercetin 80 mg/kgbw, P1: rats induced with doxorubicin 15 mg/kgbw + VAEE 100 mg/kgbw, P2: rats induced with doxorubicin 15 mg/kgbw + VAEE 200 mg/kgbw, P3: rats induced with doxorubicin 15 mg/kgbw + VAEE 400 mg/kgbw, P4: rats induced with doxorubicin 15 mg/kgbw + VAEE 600 mg/kgbw, P5: rats induced with doxorubicin 15 mg/kgbw + VAEE 800 mg/kgbw; the yellow color of the expression of TGFβ).

Article Snippet: TGFβ detection utilized TGFβ mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 in PBS, Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bcl-2 detection utilized Bcl-2 rat monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 in PBS, Santa Cruz Biotechnology, Santa Cruz, CA, USA), collagen IV detection utilized collagen IV mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 in PBS, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection utilized a monoclonal mouse anti-cytochrome C antibody (ready-to-use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and 0.09% (NaN3).

Techniques: Staining, Expressing

Journal: bioRxiv

Article Title: Development of attenuated coxsackievirus B3 vectored intranasal pre-emptive pan-coronavirus vaccine

doi: 10.1101/2023.11.22.568225

Figure Lengend Snippet: (A) Genome Schematic diagram of attenuated rCVB3 delivery vector based intranasal pre-emptive pan-coronavirus vaccines construction. (B) Cartoon models of rCVB3-EPI and rCVB3-RBD-trimer. (C) Western Blot analysis of the intranasal pre-emptive pan-coronavirus vaccines. Collection and analysis the protein in serial passages of the recombinant viruses (1-6 passages) from Vero culture cells. (D) Plaque morphology of viruses on Vero cells. For each recombinant virus, we randomly measured 10 Plaque diameter for statistical analysis. (E) Experimental schema of vaccination and sampling timeline. Nasal washes and serum were collected on day 28, n=6 /group. (F) SARS-CoV-2 S1 specific serum IgG antibodies levels on day 28, group rCVB3-RBD-trimer and rCVB3-EPI SARS-CoV-2 S1 specific serum IgG antibodies levels on day 28 compare to control group. IgG levels measured by ELISA and presented as OD450 nm values, n=4 / group. (G) The neutralizing activity of serum samples isolated from vaccinated mice (n =6 per group) against SARS-CoV-2 (Wuhan-Hu-1) pseudovirus. The vertical axis represents the pseudovirus inhibition rate of each immune serum at a serum dilution of 1:45. (H) SARS-CoV-2 S1-specific secretory IgA (sIgA) response in nasal lavage fluid of immunized mice was assessed on day 14 post booster immunization. n=6/group. (L-J) Percentages of IFN-γ + CD3 + and CD4 + splenic T cells by FCM analysis on day 14 after the last immunization. The percentage of IFN-γ + CD4 + Th cells among from splenocytes was measured under non-stimulation (NON), PMA stimulation (PMA), and SARS-CoV-2 peptide pool (PP) stimulation, respectively, for both experimental group and vaccine group (n=3/ group). (K-L) The percentage of IL-4 + Th cells and IFN-γ + Th cells among CD4 + T lymphocytes was determined under SARS-CoV-2 PP stimulation, respectively. Significance analysis was performed using a two-tailed t-test for unpaired samples. One-way analysis of variance (ANOVA) was also employed for significance analysis, data are represented as mean ± standard error of the mean (SEM) *, P<0.05; **, P<0.01; ***, P<0.001.

Article Snippet: Next, cells were fixed and permeabilized using the Fixation/Permeabilization Kit (554714, BD Pharmingen, USA) to enable intracellular staining with PE Rat Anti-Mouse IFN-γ (554412, BD Pharmingen, USA) and PE-Cy™7 Rat Anti-Mouse IL-4 (560699, BD Pharmingen, USA).

Techniques: Plasmid Preparation, Vaccines, Western Blot, Recombinant, Virus, Sampling, Enzyme-linked Immunosorbent Assay, Activity Assay, Isolation, Inhibition, Two Tailed Test