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Image Search Results
Journal: PLoS ONE
Article Title: Effect of progesterone on Smad signaling and TGF-β/Smad-regulated genes in lung epithelial cells
doi: 10.1371/journal.pone.0200661
Figure Lengend Snippet: A549 cells were incubated with TGF-β1 (10 ng/mL) and progesterone (10 μg/mL). qPCR of CTGF mRNA was performed after 12 h ( A ) and western blotting analysis after 24 h ( B ). Relative mRNA levels of CTGF were calculated by normalizing signals to detected GAPDH mRNA. Differences compared with untreated cells were calculated. Means + SD of at least n = 3 independent experiments are shown. In B , a representative immunoblot of CTGF and β-actin of n = 3 independent experiments is shown. ** p < 0.01 compared with control cells; ## p < 0.01 compared with cells treated with TGF-β1.
Article Snippet: Membranes were blocked in 5% bovine serum albumin and incubated with primary antibodies recognizing CTGF (ab6992; Abcam, Cambridge, UK), transgelin (sc-50446; Santa Cruz Biotechnology, Santa Cruz, CA), anti-Smad2/3-P (kind gift from Dr. C.-H. Heldin, Ludwig Institute for Cancer Research, Uppsala, Sweden), and
Techniques: Incubation, Western Blot
Journal: PLoS ONE
Article Title: Effect of progesterone on Smad signaling and TGF-β/Smad-regulated genes in lung epithelial cells
doi: 10.1371/journal.pone.0200661
Figure Lengend Snippet: A549 cells were incubated with TGF-β1 (10 ng/mL) and progesterone (10 μg/mL). Promoter analysis ( A ) and qPCR of transgelin ( TAGLN ) mRNA ( B ) were performed after 12 h, and western blotting analysis ( C ) after 72 h. Relative mRNA levels of TAGLN were calculated by normalizing signals to detected GAPDH mRNA ( B ) and compared with untreated cells. Means + SD of at least n = 3 independent experiments are shown. In C , a representative immunoblot of transgelin and β-actin of n = 3 independent experiments is shown. ** p < 0.01 and *** p < 0.001 compared with control cells; # p < 0.05, ## p < 0.01, and ### p < 0.001 compared with cells treated with TGF-β1.
Article Snippet: Membranes were blocked in 5% bovine serum albumin and incubated with primary antibodies recognizing CTGF (ab6992; Abcam, Cambridge, UK), transgelin (sc-50446; Santa Cruz Biotechnology, Santa Cruz, CA), anti-Smad2/3-P (kind gift from Dr. C.-H. Heldin, Ludwig Institute for Cancer Research, Uppsala, Sweden), and
Techniques: Incubation, Western Blot