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Image Search Results
Journal: Scientific reports
Article Title: A directional 3D neurite outgrowth model for studying motor axon biology and disease.
doi: 10.1038/s41598-021-81335-z
Figure Lengend Snippet: Figure 1. Motor neuron progenitors form 3D networks in the OrganoPlate. (A) Image of an OrganoPlate 3-lane. The OrganoPlate is a microfluidic platform based on a 384-well plate design containing 40 individual tissue culture chips. The image is copyright of MIMETAS BV. (B) Each tissue culture chip contains 3 microfluidic lanes that join in the center of the chip. The 3-lane architecture in the chips’ center is assured by phaseguides that enable patterning of extracellular matrix gel. Inlet and outlet wells allow access to each lane: top inlet (TI) and top outlet (TO) wells allow access to top lane; middle inlet (MI) and outlet (MO) wells to middle lane; and bottom inlet (BI) and outlet (BO) wells to the bottom lane. Matrigel-GFR without cells is added to the middle lane. Following gelation, motor neuron progenitors embedded in matrigel-GFR are seeded in the top lane. (C) Schematic overview of cell seeding procedure. Matrigel-GFR is seeded in the middle lane and MNPs embedded in matrigel-GFR are seeded in the top lane (panel 1). MNPs form networks in the top lane (panel 2), after which neurites protrude into the middle and eventually bottom lane (panel 3). Blue color indicates presence of gel, red color indicates presence of medium. (D) Schematic overview of the spatial segregation of motor neuron somata (top lane) and axons (middle and bottom lanes) in OrganoPlate 3-lane cultures.
Article Snippet: Cell culture.
Techniques:
Journal: Scientific reports
Article Title: A directional 3D neurite outgrowth model for studying motor axon biology and disease.
doi: 10.1038/s41598-021-81335-z
Figure Lengend Snippet: Figure 2. Progenitors differentiate into motor neurons and extend their axons into an adjacent gel lane. (A) MNPs were differentiated in the OrganoPlate for 17 days and immunostained for motor neuron markers SMI- 32, ISL1, and CHAT. Maximum projection images of neuronal networks in the top lane are shown, the scale bar is 200 µm. (B) mRNA expression levels of induced pluripotent stem cells (iPSC), motor neuron progenitors (MNP) and motor neurons differentiated for 17 days (MN d17) in the OrganoPlate. Expression of markers of neural stem cells (NANOG, SOX2, PAX6), motor neuron progenitors (OLIG2), motor neurons (ISL1, CHAT, RBFOX3, VACHT, NFH), mature neurons (SYN1/SYNAPSIN, MAP2) and glial cells (S100β) was evaluated. Heatmap shows fold-change values as compared to expression in iPSCs, and the graphic representation was created using GraphPad Prism, version 8.3.1 (https://www.graphpad.com/scientific-software/prism/). (C) Motor neurons (day 17) were immunostained for dendritic marker MAP2 (red) and axonal marker TAU (green). Neurites protruding into the middle lane are mostly TAU-positive and MAP2-negative, indicating a predominantly axonal nature. Maximum projection images, the scale bar is 200 µm.
Article Snippet: Cell culture.
Techniques: Expressing, Software, Marker