Journal: eLife

Article Title: Two-photon imaging in mice shows striosomes and matrix have overlapping but differential reinforcement-related responses

doi: 10.7554/elife.32353

Figure Lengend Snippet: Figure 1. Striosomes are labeled with tdTomato in Mash1-CreER;Ai14 mice that received tamoxifen at E11.5. Images illustrate two examples (rows) of striosomal labeling of cell bodies and neuropil by tdTomato (A,D, red) as verified by MOR1 immunostaining identifying striosomes (B,E, blue). Merged images show overlap of tdTomato and MOR1 labeling (C,F). Scale bars indicate 100 mm. DOI: https://doi.org/10.7554/eLife.32353.002 The following figure supplement is available for figure 1:

Article Snippet: For staining, sections were first rinsed 3 5 min in PBS-Tx (0.01 M PBS + 0.2% Triton X-100), then were incubated in blocking buffer (Perkin Elmer TSA Kit) for 20 min followed by incubation with primary antibodies for GFP (Polyclonal, chicken, Abcam ab13970, 1:2000) and MOR1 (Poly- clonal, goat, Santa Cruz sc-7488, 1:500).

Techniques: Labeling, Immunostaining

Journal: Pain

Article Title: Ex vivo non-viral gene delivery of μ-opioid receptor ( OPRM1 ) to attenuate cancer-induced pain

doi: 10.1097/j.pain.0000000000000750

Figure Lengend Snippet: (A) The transfection efficiency of plasmid DNA encoding the gene for luciferase with mTat/FH in gingival fibroblasts (HGF-1) and oral SCC cells (HSC-3). Plasmid DNA without the vector was used for control. ***p < 0.001. (B) Cell viability with the mTat/FH/GFP or mTat/FH/OPRM1-GFP complex for transfection in HSC-3 cells evaluated by MTT assay. As a control (100% viability), non-transfected cells were used. There was no significant difference among these groups (p = 0.072).

Article Snippet: Plasmid DNAs Plasmid DNA encoding for luciferase (gWIZ luciferase) and the green fluorescent protein (GFP) (gWIZ GFP) under the control of the cytomegalovirus promoter/enhancer obtained from Genlantis (San Diego, CA), and plasmid DNA encoding OPRM1-GFP (Origene, Rockville, MD) were used.

Techniques: Transfection, Plasmid Preparation, Luciferase, Control, MTT Assay

Journal: Pain

Article Title: Ex vivo non-viral gene delivery of μ-opioid receptor ( OPRM1 ) to attenuate cancer-induced pain

doi: 10.1097/j.pain.0000000000000750

Figure Lengend Snippet: Expression of GFP after transfection of plasmid DNA encoding GFP or OPRM1-GFP with a non-viral vector into oral SCC cells. (A) Green fluorescence images of non-transfected (as control), GFP plasmid transfected (as GFP), and OPRM1-GFP plasmid transfected HSC-3 cells (as OPRM1). Images are shown at 40× magnification. (B) The transfection efficiency of plasmid DNA encoding GFP or OPRM1-GFP with a non-viral vector in HSC-3 cells. Calculation is descried in Materials and Methods. There is no significant difference between GFP and OPRM1 (p = 0.97).

Article Snippet: Plasmid DNAs Plasmid DNA encoding for luciferase (gWIZ luciferase) and the green fluorescent protein (GFP) (gWIZ GFP) under the control of the cytomegalovirus promoter/enhancer obtained from Genlantis (San Diego, CA), and plasmid DNA encoding OPRM1-GFP (Origene, Rockville, MD) were used.

Techniques: Expressing, Transfection, Plasmid Preparation, Fluorescence, Control