mononuclear cells mncs Search Results


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  • 85
    Lonza fresh human bone marrow mononuclear cells
    The specificity of mAb/Saporin-Ab for TCblR was demonstarted by adding purified soluble TCblR to the culture medium, which resulted in decreased availability of mAb/Saporin to receptors on <t>cells</t> and decreased cell death (Panel A). The inhibitory effect of the mAb-Saporin Ab also decreased when excess primary mAb or normal mouse IgG was added to the culture. No inhibition of cell growth was observed when saporin-Ab was withheld or when primary mAb was withheld (Panel B). Values are expressed as percentage of maximum inhibition shown in the first bar. Panel C shows the specificity of mAb-Sap-Ab for cancer cells and lack of toxicity to primary skin fibroblasts. Panel D shows the effect of mAb-SapAb on <t>human</t> <t>bone</t> <t>marrow</t> cells in vitro.
    Fresh Human Bone Marrow Mononuclear Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher mononuclear cell mnc complete medium complete medium mnc basal medium complete medium mnc basal medium imdm
    The specificity of mAb/Saporin-Ab for TCblR was demonstarted by adding purified soluble TCblR to the culture medium, which resulted in decreased availability of mAb/Saporin to receptors on <t>cells</t> and decreased cell death (Panel A). The inhibitory effect of the mAb-Saporin Ab also decreased when excess primary mAb or normal mouse IgG was added to the culture. No inhibition of cell growth was observed when saporin-Ab was withheld or when primary mAb was withheld (Panel B). Values are expressed as percentage of maximum inhibition shown in the first bar. Panel C shows the specificity of mAb-Sap-Ab for cancer cells and lack of toxicity to primary skin fibroblasts. Panel D shows the effect of mAb-SapAb on <t>human</t> <t>bone</t> <t>marrow</t> cells in vitro.
    Mononuclear Cell Mnc Complete Medium Complete Medium Mnc Basal Medium Complete Medium Mnc Basal Medium Imdm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore mononuclear cells mnc
    The specificity of mAb/Saporin-Ab for TCblR was demonstarted by adding purified soluble TCblR to the culture medium, which resulted in decreased availability of mAb/Saporin to receptors on <t>cells</t> and decreased cell death (Panel A). The inhibitory effect of the mAb-Saporin Ab also decreased when excess primary mAb or normal mouse IgG was added to the culture. No inhibition of cell growth was observed when saporin-Ab was withheld or when primary mAb was withheld (Panel B). Values are expressed as percentage of maximum inhibition shown in the first bar. Panel C shows the specificity of mAb-Sap-Ab for cancer cells and lack of toxicity to primary skin fibroblasts. Panel D shows the effect of mAb-SapAb on <t>human</t> <t>bone</t> <t>marrow</t> cells in vitro.
    Mononuclear Cells Mnc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Nycomed mononuclear cells mnc
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Mononuclear Cells Mnc, supplied by Nycomed, used in various techniques. Bioz Stars score: 91/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA mononuclear cells mncs
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Mononuclear Cells Mncs, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    GE Healthcare mononuclear cells mncs
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Mononuclear Cells Mncs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 747 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore mononuclear cells mncs
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Mononuclear Cells Mncs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 470 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore human cord blood mononuclear cells mncs
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Human Cord Blood Mononuclear Cells Mncs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Becton Dickinson peripheral blood mononuclear cells pb mncs
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Peripheral Blood Mononuclear Cells Pb Mncs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Terumo BCT blood mononuclear cell collection program cobe spectra mnc program
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Blood Mononuclear Cell Collection Program Cobe Spectra Mnc Program, supplied by Terumo BCT, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare mononuclear cells mnc
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Mononuclear Cells Mnc, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Ficoll-Paque Pharmacia mononuclear cells mncs
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Mononuclear Cells Mncs, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare 150 200 g mononuclear cells mncs
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    150 200 G Mononuclear Cells Mncs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Biochrom peripheral blood mononuclear cells pb mncs
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Peripheral Blood Mononuclear Cells Pb Mncs, supplied by Biochrom, used in various techniques. Bioz Stars score: 85/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Axis-Shield Diagnostics peripheral blood mononuclear cells mncs
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Peripheral Blood Mononuclear Cells Mncs, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Lonza peripheral blood mononuclear cells mncs
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Peripheral Blood Mononuclear Cells Mncs, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The specificity of mAb/Saporin-Ab for TCblR was demonstarted by adding purified soluble TCblR to the culture medium, which resulted in decreased availability of mAb/Saporin to receptors on cells and decreased cell death (Panel A). The inhibitory effect of the mAb-Saporin Ab also decreased when excess primary mAb or normal mouse IgG was added to the culture. No inhibition of cell growth was observed when saporin-Ab was withheld or when primary mAb was withheld (Panel B). Values are expressed as percentage of maximum inhibition shown in the first bar. Panel C shows the specificity of mAb-Sap-Ab for cancer cells and lack of toxicity to primary skin fibroblasts. Panel D shows the effect of mAb-SapAb on human bone marrow cells in vitro.

    Journal: Molecular cancer therapeutics

    Article Title: Targeted Delivery of Saporin Toxin by Monoclonal Antibody to the Transcobalamin Receptor, TCblR/CD320

    doi: 10.1158/1535-7163.MCT-10-0513

    Figure Lengend Snippet: The specificity of mAb/Saporin-Ab for TCblR was demonstarted by adding purified soluble TCblR to the culture medium, which resulted in decreased availability of mAb/Saporin to receptors on cells and decreased cell death (Panel A). The inhibitory effect of the mAb-Saporin Ab also decreased when excess primary mAb or normal mouse IgG was added to the culture. No inhibition of cell growth was observed when saporin-Ab was withheld or when primary mAb was withheld (Panel B). Values are expressed as percentage of maximum inhibition shown in the first bar. Panel C shows the specificity of mAb-Sap-Ab for cancer cells and lack of toxicity to primary skin fibroblasts. Panel D shows the effect of mAb-SapAb on human bone marrow cells in vitro.

    Article Snippet: Fresh human bone marrow mononuclear cells were obtained from Lonza, Walkersville, MD.

    Techniques: Purification, Inhibition, In Vitro

    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P

    Journal: Clinical and Experimental Immunology

    Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

    doi: 10.1111/j.1365-2249.2004.02556.x

    Figure Lengend Snippet: MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P

    Article Snippet: Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway).

    Techniques: Centrifugation, Enzyme-linked Immunosorbent Assay

    Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.

    Journal: Clinical and Experimental Immunology

    Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

    doi: 10.1111/j.1365-2249.2004.02556.x

    Figure Lengend Snippet: Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.

    Article Snippet: Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway).

    Techniques: Expressing, Centrifugation, Staining, Flow Cytometry, Cytometry

    Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.

    Journal: Clinical and Experimental Immunology

    Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

    doi: 10.1111/j.1365-2249.2004.02556.x

    Figure Lengend Snippet: Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.

    Article Snippet: Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway).

    Techniques: Centrifugation, Staining, Flow Cytometry, Cytometry