Journal: Blood and Lymphatic Cancer: Targets and Therapy

Article Title: Enzymatic Laccase Nanoreactors Induce Apoptosis in MOLT-4-ALL Cells and Activate Prodrugs in a Synergetic Effect

doi: 10.2147/BLCTT.S576292

Figure Lengend Snippet: Curve dose-response on MOLT-4 leukemia model cells. ( A ) Pro-drugs and ( B ) laccase from C. gallica .

Article Snippet: The MOLT-4 cell line, derived from human T cells originating from acute lymphoblastic leukemia, was obtained from a certified cell bank (ATCC, MOLT-4-CRL-1582).

Techniques:

Journal: Blood and Lymphatic Cancer: Targets and Therapy

Article Title: Enzymatic Laccase Nanoreactors Induce Apoptosis in MOLT-4-ALL Cells and Activate Prodrugs in a Synergetic Effect

doi: 10.2147/BLCTT.S576292

Figure Lengend Snippet: Flow cytometry analysis to determine cell viability. The green zone indicates the death cells with a stain-positive PI, and the red zone indicates the alive cell with a stain-positive FDA. Every panel corresponds to a different treatment of MOLT-4-ALL cell: ( A ) Doxorubicin, ( B ) Doxorubicin + Lac, ( C ) Doxorubicin + VLP-saLac, ( D ) Irinotecan, ( E ) Irinotecan + Lac, ( F ) Irinotecan + VLP-saLac; ( G ) Procarbazine, ( H ) Procarbazine + Lac, ( I ) Procarbazine + VLP-saLac. The percentage in every square indicates the percentage of total events.

Article Snippet: The MOLT-4 cell line, derived from human T cells originating from acute lymphoblastic leukemia, was obtained from a certified cell bank (ATCC, MOLT-4-CRL-1582).

Techniques: Flow Cytometry, Staining

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Sesamin Acts as Anti-leukemic Compound Interacting with Novel Phosphoprotein Targets and Inducing Apoptosis in Leukemic Cells

doi: 10.22088/IJMCM.BUMS.11.1.1

Figure Lengend Snippet: Cytotoxic effects of sesamin on (a) MOLT-4 and (b) NB4 cell line after 24 hour and 48 hour treatments compare with PBMC cells after 48 hour treatment. The results showed the percentage of cell inhibition measured by MTT assay. IC 50 at 48 hours for MOLT-4 and NB4 were reported as 104.84 and 121.00 µg/mL, respectively. Data were expressed as the mean±SD of independently three experiments. The differences between sesamin treated and untreated were examined for the statistical significance: *, p < 0.05; **, p < 0.01; ***, p < 0.001

Article Snippet: Leukemic Cell culture Acute lymphoblastic leukemia (ALL) MOLT-4 and acute promyelocytic leukemia (AML) NB4 cells purchased from Cell Lines Service GmbH (Eppelheim, Germany) were cultured in 10% fetal bovine serum and 2% penicillin-streptomycin supplements in RPMI-1640 medium at the condition of 37 o C and 5% CO 2 .

Techniques: Inhibition, MTT Assay

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Sesamin Acts as Anti-leukemic Compound Interacting with Novel Phosphoprotein Targets and Inducing Apoptosis in Leukemic Cells

doi: 10.22088/IJMCM.BUMS.11.1.1

Figure Lengend Snippet: The relative normalized expression of apoptotic genes in (a) MOLT-4 and (b) NB4 after sesamin treatment at IC 50 values of 100 and 120 µg/mL, respectively, for 48 hours. The experiment was determined by Real-time PCR. Data were expressed as the mean±SEM of three independent experiments. The differences between sesamin treated and untreated were examined for the statistical significance: *, p < 0.05; **, p < 0.01

Article Snippet: Leukemic Cell culture Acute lymphoblastic leukemia (ALL) MOLT-4 and acute promyelocytic leukemia (AML) NB4 cells purchased from Cell Lines Service GmbH (Eppelheim, Germany) were cultured in 10% fetal bovine serum and 2% penicillin-streptomycin supplements in RPMI-1640 medium at the condition of 37 o C and 5% CO 2 .

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Sesamin Acts as Anti-leukemic Compound Interacting with Novel Phosphoprotein Targets and Inducing Apoptosis in Leukemic Cells

doi: 10.22088/IJMCM.BUMS.11.1.1

Figure Lengend Snippet: Venn diagram shows a set of 14 phosphoproteins present only in sesamin treated MOLT-4 (blue) and a different set of 14 phosphoproteins present only in sesamin treated NB4 (yellow)

Article Snippet: Leukemic Cell culture Acute lymphoblastic leukemia (ALL) MOLT-4 and acute promyelocytic leukemia (AML) NB4 cells purchased from Cell Lines Service GmbH (Eppelheim, Germany) were cultured in 10% fetal bovine serum and 2% penicillin-streptomycin supplements in RPMI-1640 medium at the condition of 37 o C and 5% CO 2 .

Techniques:

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Sesamin Acts as Anti-leukemic Compound Interacting with Novel Phosphoprotein Targets and Inducing Apoptosis in Leukemic Cells

doi: 10.22088/IJMCM.BUMS.11.1.1

Figure Lengend Snippet: Heatmap of phosphoproteins with significant difference in expression of (A) MOLT-4 and (B) NB4 treated with sesamin in various times compare with the control. The color indicates the expression level from low (green) to high (red). The blue arrow indicates the selected phosphoproteins

Article Snippet: Leukemic Cell culture Acute lymphoblastic leukemia (ALL) MOLT-4 and acute promyelocytic leukemia (AML) NB4 cells purchased from Cell Lines Service GmbH (Eppelheim, Germany) were cultured in 10% fetal bovine serum and 2% penicillin-streptomycin supplements in RPMI-1640 medium at the condition of 37 o C and 5% CO 2 .

Techniques: Expressing

Journal: International Journal of Molecular and Cellular Medicine

Article Title: Sesamin Acts as Anti-leukemic Compound Interacting with Novel Phosphoprotein Targets and Inducing Apoptosis in Leukemic Cells

doi: 10.22088/IJMCM.BUMS.11.1.1

Figure Lengend Snippet: The schematic diagram shows the interactions between sesamin, caspase-3 (CASP3), caspase-7 (CASP7), caspase-8 (CASP8), caspase-9 (CASP9), BCL-2, and selected phosphoprotein; PARP4 and IPPK, in MOLT-4 and NB4 leukemic cell lines using STITCH database. The round shape represents protein. The oval shape represents chemical. Red circle indicates selective proteins. Red dashed circle indicates selective chemicals

Article Snippet: Leukemic Cell culture Acute lymphoblastic leukemia (ALL) MOLT-4 and acute promyelocytic leukemia (AML) NB4 cells purchased from Cell Lines Service GmbH (Eppelheim, Germany) were cultured in 10% fetal bovine serum and 2% penicillin-streptomycin supplements in RPMI-1640 medium at the condition of 37 o C and 5% CO 2 .

Techniques:

Journal: PLoS ONE

Article Title: Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation

doi: 10.1371/journal.pone.0148137

Figure Lengend Snippet: (A) Bars show S1P receptors mRNA expression analyzed by real time quantitative PCR, compared with the control Abelson (Abl) gene (2 -ΔCt ) in T-ALL (n = 1–2, with 3–6 biological replicates). (B) Bars shows S1P1 or (C) S1P3 mRNA expression analyzed by real time quantitative PCR, compared with the control Abelson (Abl). Fold change analysis were done using HPB-ALL as calibrator to normalize the expression of the receptors on the other T-ALL blasts. Statistical analysis was made with ΔCt values and significant differences are related to HPB-ALL cells. Results are expressed as mean ± SEM and were analyzed by Student’s t test and differences were considered statistically significant when p<0.05 (*), p< 0.01 (**) or p< 0.001(***) (n = 1–2, with 3–6 biological replicates).

Article Snippet: Besides S1P1, S1P-migrating T-ALL cell lines (MOLT-4, JURKAT and CEM) also expressed S1P3 , raising the possibility that S1P3 might be involved in cell migratory responses.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control

Journal: PLoS ONE

Article Title: Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation

doi: 10.1371/journal.pone.0148137

Figure Lengend Snippet: (A) CEM cells were serum-starved for 2 h and pre-treated or not with W146 (100 μM) and/or BM-241 (100 μM). Cells were applied in Transwell™ chambers containing different S1P concentrations and incubated for 4 hours. Black bars correspond to pre-treatment with RPMI-BSA 0.1%; white bars correspond to pre-treatment with W146; grid bars correspond to pre-treatment with BML-241; and chess bars correspond to pre-treatment with W146 plus BML-241. Results were analyzed by Two-way ANOVA, followed by Bonferroni post-test (n = 3). (B) S1P receptors mRNA expression were analyzed by real time quantitative PCR, compared with the control Abelson (Abl) gene (2 -ΔCt ) in SU-DHL-1 cells (n = 4). (C) S1P1 and S1P3 mRNA expression was analyzed by real time quantitative PCR, compared with the control Abelson (Abl). Fold change analysis were done using HPB-ALL as calibrator to normalize the expression of S1P1 and S1P3 on SU-DHL-1 cells. Statistical analysis was made with ΔCt values and significant differences are related to HPB-ALL cells. Results were analyzed by Student’s t test (n = 4). (D) SU-DHL-1 cells were serum-starved for 2 h, applied to Transwell™ chambers containing different S1P concentrations and incubated for 4 hours. Results were analyzed by One-way ANOVA, followed by Tukey post-test (n = 3). (E) SU-DHL-1 cells were serum-starved for 2 h and treated or not with W146 (100 μM). Cells were applied to Transwell™ chambers containing different S1P concentrations and incubated for 4 hours. Values correspond to specific migration after subtracting the numbers of migrating cells in culture medium only. Black bars correspond to pre-treatment with RPMI-BSA 0.1% alone and white bars correspond to pre-treatment with W146. Results were analyzed by unpaired Student’s t test (n = 3). Results are expressed as mean ± SEM and differences were considered statistically significant when * p˂0.05, ** p ˂0.01 or *** p ˂0.001.

Article Snippet: Besides S1P1, S1P-migrating T-ALL cell lines (MOLT-4, JURKAT and CEM) also expressed S1P3 , raising the possibility that S1P3 might be involved in cell migratory responses.

Techniques: Incubation, Expressing, Real-time Polymerase Chain Reaction, Control, Migration

Journal: PLoS ONE

Article Title: Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation

doi: 10.1371/journal.pone.0148137

Figure Lengend Snippet: (A) S1P-induced chemotaxis: cells expressing S1P1 migrate toward low S1P concentrations (10–100 nM) and migration is inhibited when S1P1 is blocked with W146. In contrast, cells do not migrate toward high S1P concentrations (500–10000 nM), unless when S1P1 is blocked. (B) S1P-induced fugetaxis: cells expressing S1P1 do not migrate away from low S1P concentrations and the nonspecific migration observed is even smaller when S1P1 is blocked. However, cells migrate away from high S1P concentrations and migration is impaired when S1P1 is blocked.

Article Snippet: Besides S1P1, S1P-migrating T-ALL cell lines (MOLT-4, JURKAT and CEM) also expressed S1P3 , raising the possibility that S1P3 might be involved in cell migratory responses.

Techniques: Chemotaxis Assay, Expressing, Migration