Journal: Materials Today Bio

Article Title: Multifunctional nanomedicine targeting the 'seed-and-soil' of hair follicles via simultaneous alleviation of oxidative stress and activation of autophagy for androgenetic alopecia therapy

doi: 10.1016/j.mtbio.2025.102145

Figure Lengend Snippet: Synthesis and characterization of TFC. (A) Particle size distribution of TFC. Inset: Appearance of TFC solution. (B) Transmission electron microscopy (TEM) image of TFC. (C) Energy dispersive spectroscopy (EDS) spectrum of TFC. (D) UV–visible absorption spectra of TFC, Cur, and TA. (E) Infrared (IR) absorption spectra of TFC, Cur, and TA. (F) Molecular dynamics simulation of the self-assembly mechanism of TFC. (G) X-ray diffraction (XRD) patterns of TFC, Cur, TA, and TA/Cur mixtures. (H) X-ray photoelectron spectroscopy (XPS) spectrum of TFC. (I) XPS fine spectrum of Fe in TFC. (J) Particle size colloidal stability of TFC over 72 h in various solutions (n = 3).

Article Snippet: Inset: Appearance of TFC solution. (B) Transmission electron microscopy (TEM) image of TFC. (C) Energy dispersive spectroscopy (EDS) spectrum of TFC. (D) UV–visible absorption spectra of TFC, Cur, and TA. (E) Infrared (IR) absorption spectra of TFC, Cur, and TA. (F) Molecular dynamics simulation of the self-assembly mechanism of TFC. (G) X-ray diffraction (XRD) patterns of TFC, Cur, TA, and TA/Cur mixtures. (H) X-ray photoelectron spectroscopy (XPS) spectrum of TFC. (I) XPS fine spectrum of Fe in TFC. (J) Particle size colloidal stability of TFC over 72 h in various solutions (n = 3).

Techniques: Transmission Assay, Electron Microscopy, Spectroscopy

Journal: Materials Today Bio

Article Title: Multifunctional nanomedicine targeting the 'seed-and-soil' of hair follicles via simultaneous alleviation of oxidative stress and activation of autophagy for androgenetic alopecia therapy

doi: 10.1016/j.mtbio.2025.102145

Figure Lengend Snippet: Intracellular free radical scavenging activity of TFC. (A) Cell viability of DPCs treated with different concentrations of TFC for 24 h (n = 3). (B) Confocal fluorescence images showing the cellular uptake of TFC in DPCs at different time points. (C) Flow cytometry measurement of fluorescence intensity to quantify TFC uptake by DPCs and (D) quantitative analysis (n = 3). (E) Fluorescence microscopy images showing the effect of TFC on the levels of various intracellular RONS in human dermal papilla cells after H 2 O 2 -induced oxidative stress. (F) Quantitative analysis of total RONS scavenging by TFC. Quantitative analysis of TFC-mediated scavenging of (G) superoxide anions (•O 2 − ), (H) hydroxyl radicals (•OH)/peroxynitrite (ONOO − ), and (I) nitric oxide (•NO) (n = 3). (J) MDA level showing the ability of TFC to alleviate oxidative stress (n = 3).

Article Snippet: Inset: Appearance of TFC solution. (B) Transmission electron microscopy (TEM) image of TFC. (C) Energy dispersive spectroscopy (EDS) spectrum of TFC. (D) UV–visible absorption spectra of TFC, Cur, and TA. (E) Infrared (IR) absorption spectra of TFC, Cur, and TA. (F) Molecular dynamics simulation of the self-assembly mechanism of TFC. (G) X-ray diffraction (XRD) patterns of TFC, Cur, TA, and TA/Cur mixtures. (H) X-ray photoelectron spectroscopy (XPS) spectrum of TFC. (I) XPS fine spectrum of Fe in TFC. (J) Particle size colloidal stability of TFC over 72 h in various solutions (n = 3).

Techniques: Activity Assay, Fluorescence, Flow Cytometry, Microscopy

Journal: Materials Today Bio

Article Title: Multifunctional nanomedicine targeting the 'seed-and-soil' of hair follicles via simultaneous alleviation of oxidative stress and activation of autophagy for androgenetic alopecia therapy

doi: 10.1016/j.mtbio.2025.102145

Figure Lengend Snippet: The cellular protective effect of TFC under oxidative stress. (A) The effect of TFC on cell viability following H 2 O 2 treatment at different concentrations under oxidative stress conditions (n = 3). (B) Fluorescence microscopy images of live (green) and dead (red) cells following H 2 O 2 treatment and TFC rescue. (C) Quantification of the live/dead cell ratio (n = 3). (D) Fluorescence microscopy images showing autophagic structures in DPCs after treatment with TFC. (E) Transmission electron microscopy images showing autophagic vesicles in DPCs after treatment with TFC. (F) Western blot analysis of LC3 expression in DPCs after treatment with TFC. (G) Semi-quantitative analysis of LC3 II expression in DPCs (n = 3). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Inset: Appearance of TFC solution. (B) Transmission electron microscopy (TEM) image of TFC. (C) Energy dispersive spectroscopy (EDS) spectrum of TFC. (D) UV–visible absorption spectra of TFC, Cur, and TA. (E) Infrared (IR) absorption spectra of TFC, Cur, and TA. (F) Molecular dynamics simulation of the self-assembly mechanism of TFC. (G) X-ray diffraction (XRD) patterns of TFC, Cur, TA, and TA/Cur mixtures. (H) X-ray photoelectron spectroscopy (XPS) spectrum of TFC. (I) XPS fine spectrum of Fe in TFC. (J) Particle size colloidal stability of TFC over 72 h in various solutions (n = 3).

Techniques: Fluorescence, Microscopy, Transmission Assay, Electron Microscopy, Western Blot, Expressing

Journal: Materials Today Bio

Article Title: Multifunctional nanomedicine targeting the 'seed-and-soil' of hair follicles via simultaneous alleviation of oxidative stress and activation of autophagy for androgenetic alopecia therapy

doi: 10.1016/j.mtbio.2025.102145

Figure Lengend Snippet: Preparation and characterization of TFC MN. (A) Schematic diagram of the preparation process for TFC MN. (B) Visual appearance of the TFC MN patch. (C) Microscopic image showing the structure of the TFC MN array. (D) Scanning electron microscopy (SEM) image of the TFC MN array. (E) Mechanical performance testing curve showing the force-displacement behavior of TFC MN. (F) TFC MN applied to ex vivo mouse skin after 5 min. (G) Image of ex vivo mouse skin treated with TFC MN, showing punctate holes corresponding to MN insertion. (H) Hematoxylin and eosin (H&E) staining of mouse skin post-TFC MN treatment.

Article Snippet: Inset: Appearance of TFC solution. (B) Transmission electron microscopy (TEM) image of TFC. (C) Energy dispersive spectroscopy (EDS) spectrum of TFC. (D) UV–visible absorption spectra of TFC, Cur, and TA. (E) Infrared (IR) absorption spectra of TFC, Cur, and TA. (F) Molecular dynamics simulation of the self-assembly mechanism of TFC. (G) X-ray diffraction (XRD) patterns of TFC, Cur, TA, and TA/Cur mixtures. (H) X-ray photoelectron spectroscopy (XPS) spectrum of TFC. (I) XPS fine spectrum of Fe in TFC. (J) Particle size colloidal stability of TFC over 72 h in various solutions (n = 3).

Techniques: Electron Microscopy, Ex Vivo, Staining

Journal: Materials Today Bio

Article Title: Multifunctional nanomedicine targeting the 'seed-and-soil' of hair follicles via simultaneous alleviation of oxidative stress and activation of autophagy for androgenetic alopecia therapy

doi: 10.1016/j.mtbio.2025.102145

Figure Lengend Snippet: In vivo pharmacological evaluations of TFC MN. (A) Photographs showing hair regeneration in mice from each group at days 1, 7, 14, 21, and 28 post-depilation. (B) Number of days after depilation for the skin of mice in each group to turn black (n = 5). (C) Hair length measurements of newly formed hair in each group of mice on day 28 post-depilation (n = 5). (D) Hematoxylin and eosin (H&E) staining of mouse skin on day 14 post-depilation. (E) Quantitative measurement of hair follicle length in the skin of mice in each group on day 14 post-depilation (n = 3). (F) Epidermis thickness and (G) dermis thickness in the skin of mice in each group on day 14 post-depilation (n = 3). (H) Fluorescence microscopy images of reactive oxygen species (ROS), Ki67, CD31, and LC3B expression in skin tissue on day 14 post-depilation. (I) Quantification of superoxide anion levels using DHE fluorescence intensity (n = 3). (J) Quantification of Ki67 fluorescence intensity as an indicator of cell proliferation (n = 3). (K) Quantification of CD31 fluorescence intensity as a marker of angiogenesis (n = 3). (L) Quantification of LC3B fluorescence intensity as a marker of autophagy (n = 3). Blue: DAPI; Red: DHE, Ki67, CD31, LC3B. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Inset: Appearance of TFC solution. (B) Transmission electron microscopy (TEM) image of TFC. (C) Energy dispersive spectroscopy (EDS) spectrum of TFC. (D) UV–visible absorption spectra of TFC, Cur, and TA. (E) Infrared (IR) absorption spectra of TFC, Cur, and TA. (F) Molecular dynamics simulation of the self-assembly mechanism of TFC. (G) X-ray diffraction (XRD) patterns of TFC, Cur, TA, and TA/Cur mixtures. (H) X-ray photoelectron spectroscopy (XPS) spectrum of TFC. (I) XPS fine spectrum of Fe in TFC. (J) Particle size colloidal stability of TFC over 72 h in various solutions (n = 3).

Techniques: In Vivo, Staining, Fluorescence, Microscopy, Expressing, Marker

Journal: BMC Cancer

Article Title: Exploring the mutational spectrum of key kinase genes PIK3CA , BRAF , EGFR , ALK and ROS1 in oral squamous cell carcinoma

doi: 10.1186/s12885-025-14609-8

Figure Lengend Snippet: Analysis of Wild and Mutant EGFR p.R521K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I )

Article Snippet: Fig. 7 Analysis of Wild and Mutant EGFR p.R521K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 8 Analysis of Wild and Mutant EGFR p.R831C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 9 Analysis of Wild and Mutant ROS1 p.S2229C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 10 Analysis of Wild and Mutant ROS1 p.E1902K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 11 Analysis of Wild and Mutant ROS1 p.K2228Q Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 12 Analysis of Wild and Mutant ROS1 p.P221S Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 13 Analysis of Wild and Mutant ROS1 p.D2213N Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 14 Average Rg values of interacting sites mutations for EGFR and ROS1 (Both WT and MT)

Techniques: Mutagenesis

Journal: BMC Cancer

Article Title: Exploring the mutational spectrum of key kinase genes PIK3CA , BRAF , EGFR , ALK and ROS1 in oral squamous cell carcinoma

doi: 10.1186/s12885-025-14609-8

Figure Lengend Snippet: Analysis of Wild and Mutant EGFR p.R831C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I )

Article Snippet: Fig. 7 Analysis of Wild and Mutant EGFR p.R521K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 8 Analysis of Wild and Mutant EGFR p.R831C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 9 Analysis of Wild and Mutant ROS1 p.S2229C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 10 Analysis of Wild and Mutant ROS1 p.E1902K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 11 Analysis of Wild and Mutant ROS1 p.K2228Q Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 12 Analysis of Wild and Mutant ROS1 p.P221S Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 13 Analysis of Wild and Mutant ROS1 p.D2213N Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 14 Average Rg values of interacting sites mutations for EGFR and ROS1 (Both WT and MT)

Techniques: Mutagenesis

Journal: BMC Cancer

Article Title: Exploring the mutational spectrum of key kinase genes PIK3CA , BRAF , EGFR , ALK and ROS1 in oral squamous cell carcinoma

doi: 10.1186/s12885-025-14609-8

Figure Lengend Snippet: Analysis of Wild and Mutant ROS1 p.S2229C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I )

Article Snippet: Fig. 7 Analysis of Wild and Mutant EGFR p.R521K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 8 Analysis of Wild and Mutant EGFR p.R831C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 9 Analysis of Wild and Mutant ROS1 p.S2229C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 10 Analysis of Wild and Mutant ROS1 p.E1902K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 11 Analysis of Wild and Mutant ROS1 p.K2228Q Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 12 Analysis of Wild and Mutant ROS1 p.P221S Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 13 Analysis of Wild and Mutant ROS1 p.D2213N Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 14 Average Rg values of interacting sites mutations for EGFR and ROS1 (Both WT and MT)

Techniques: Mutagenesis

Journal: BMC Cancer

Article Title: Exploring the mutational spectrum of key kinase genes PIK3CA , BRAF , EGFR , ALK and ROS1 in oral squamous cell carcinoma

doi: 10.1186/s12885-025-14609-8

Figure Lengend Snippet: Analysis of Wild and Mutant ROS1 p.E1902K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I )

Article Snippet: Fig. 7 Analysis of Wild and Mutant EGFR p.R521K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 8 Analysis of Wild and Mutant EGFR p.R831C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 9 Analysis of Wild and Mutant ROS1 p.S2229C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 10 Analysis of Wild and Mutant ROS1 p.E1902K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 11 Analysis of Wild and Mutant ROS1 p.K2228Q Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 12 Analysis of Wild and Mutant ROS1 p.P221S Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 13 Analysis of Wild and Mutant ROS1 p.D2213N Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 14 Average Rg values of interacting sites mutations for EGFR and ROS1 (Both WT and MT)

Techniques: Mutagenesis

Journal: BMC Cancer

Article Title: Exploring the mutational spectrum of key kinase genes PIK3CA , BRAF , EGFR , ALK and ROS1 in oral squamous cell carcinoma

doi: 10.1186/s12885-025-14609-8

Figure Lengend Snippet: Analysis of Wild and Mutant ROS1 p.K2228Q Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I )

Article Snippet: Fig. 7 Analysis of Wild and Mutant EGFR p.R521K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 8 Analysis of Wild and Mutant EGFR p.R831C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 9 Analysis of Wild and Mutant ROS1 p.S2229C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 10 Analysis of Wild and Mutant ROS1 p.E1902K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 11 Analysis of Wild and Mutant ROS1 p.K2228Q Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 12 Analysis of Wild and Mutant ROS1 p.P221S Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 13 Analysis of Wild and Mutant ROS1 p.D2213N Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 14 Average Rg values of interacting sites mutations for EGFR and ROS1 (Both WT and MT)

Techniques: Mutagenesis

Journal: BMC Cancer

Article Title: Exploring the mutational spectrum of key kinase genes PIK3CA , BRAF , EGFR , ALK and ROS1 in oral squamous cell carcinoma

doi: 10.1186/s12885-025-14609-8

Figure Lengend Snippet: Analysis of Wild and Mutant ROS1 p.P221S Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I )

Article Snippet: Fig. 7 Analysis of Wild and Mutant EGFR p.R521K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 8 Analysis of Wild and Mutant EGFR p.R831C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 9 Analysis of Wild and Mutant ROS1 p.S2229C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 10 Analysis of Wild and Mutant ROS1 p.E1902K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 11 Analysis of Wild and Mutant ROS1 p.K2228Q Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 12 Analysis of Wild and Mutant ROS1 p.P221S Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 13 Analysis of Wild and Mutant ROS1 p.D2213N Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 14 Average Rg values of interacting sites mutations for EGFR and ROS1 (Both WT and MT)

Techniques: Mutagenesis

Journal: BMC Cancer

Article Title: Exploring the mutational spectrum of key kinase genes PIK3CA , BRAF , EGFR , ALK and ROS1 in oral squamous cell carcinoma

doi: 10.1186/s12885-025-14609-8

Figure Lengend Snippet: Analysis of Wild and Mutant ROS1 p.D2213N Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I )

Article Snippet: Fig. 7 Analysis of Wild and Mutant EGFR p.R521K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 8 Analysis of Wild and Mutant EGFR p.R831C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 9 Analysis of Wild and Mutant ROS1 p.S2229C Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 10 Analysis of Wild and Mutant ROS1 p.E1902K Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 11 Analysis of Wild and Mutant ROS1 p.K2228Q Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 12 Analysis of Wild and Mutant ROS1 p.P221S Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 13 Analysis of Wild and Mutant ROS1 p.D2213N Using Gromacs Molecular Dynamics Simulations ( A - G ) and Ramachandran Plot Profiles ( H - I ) Fig. 14 Average Rg values of interacting sites mutations for EGFR and ROS1 (Both WT and MT)

Techniques: Mutagenesis