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Image Search Results
Journal: Stem cell research
Article Title: Significant therapeutic effects of adult human multipotent neural cells on spinal cord injury.
doi: 10.1016/j.scr.2018.07.006
Figure Lengend Snippet: Fig. 3. In vivo fate of transplanted ahMNCs in injured spinal cords. Transplanted ahMNCs were traced by immuno- fluorescent staining against human cytoplasm. The results using 001TL ahMNCs were presented. In the experiments using 008TL, similar results were ob- served. (A) Representative image showed human cytoplasm-positive cells in the spinal cord. Scale bar = 20 μm (B) The total number of human cyto- plasm-positive cells decreased continuously after transplantation of ahMNCs. Average ± SEM. (C) The portion of each neural cell-types was determined at 3, 6, and 12 days after injection. The portions of NSCs, neurons, astrocytes, and oligodendrocytes were determined by Nestin-positive, Tuj1-positive, GFAP-positive, and MOG-positive cells among human cytoplasm-positive cells. (D) Representative images showed cells double-positive for each marker and human cytoplasm in the spinal cord after transplantation of ahMNCs. Scale bar = 20 μm.
Article Snippet: Slides were treated with primary antibodies overnight at 4 °C; anti-human specific cytoplasmic protein (Stem Cells), Nestin (Novus), Tuj1 (Abcam), GFAP (Abcam),
Techniques: In Vivo, Staining, Transplantation Assay, Injection, Marker