mntbap Search Results


mntbap  (MedChemExpress)
93
MedChemExpress mntbap
Mntbap, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mntbap chloride  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology mntbap chloride
Mntbap Chloride, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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manganese(iii)meso-tetrakis(4-benzoic acid)porphyrin (mntbap  (Enzo Biochem)
90
Enzo Biochem manganese(iii)meso-tetrakis(4-benzoic acid)porphyrin (mntbap
Manganese(Iii)Meso Tetrakis(4 Benzoic Acid)Porphyrin (Mntbap, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sod mimetics mn(iii)tetrakis(1-methyl-4-pyridyl)porphyrin (mntmpyd)  (Mimetics)
90
Mimetics sod mimetics mn(iii)tetrakis(1-methyl-4-pyridyl)porphyrin (mntmpyd)
Sod Mimetics Mn(Iii)Tetrakis(1 Methyl 4 Pyridyl)Porphyrin (Mntmpyd), supplied by Mimetics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mntbap  (Enzo Biochem)
90
Enzo Biochem mntbap
Mntbap, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mntbap - by Bioz Stars, 2026-02
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ros scavengers mntbap  (Adipogen)
90
Adipogen ros scavengers mntbap
<t>ROS-MK2</t> signaling is not involved in OA-induced Beclin 1 Ser-90 phosphorylation. A and B, HeLa cells were pretreated with or without ROS scavengers, <t>MnTBAP</t> (10 μm) and EUK134 (10 μm), for 30 min and treated with OA (100 nm) for 6 h. A, the level of ROS production was analyzed by a total ROS detection kit. BF, bright field. B, the level of Beclin 1 Ser-90 phosphorylation was analyzed by immunoblotting. Representative images from two and three independent experiments are shown. C, HeLa cells were treated with anisomycin (25 μg/ml) for 30 min or OA (100 nm) for 6 h. The levels of MK2 activation (shown by a band shift), Beclin 1 Ser-90 phosphorylation, and HSP27 Ser-82 phosphorylation were analyzed by immunoblotting. Representative images from three independent experiments are shown. D, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the AMPK activator 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) (1 mm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated (IP), and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Representative images from three independent experiments are shown. E, 293T cells were transfected with empty plasmid or FLAG-tagged constitutively active (CA) AMPK. Beclin 1 Ser-90 and raptor Ser-792 phosphorylation was analyzed by immunoblotting. Representative images from two independent experiments are shown. F, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the mTORC1 inhibitor rapamycin (100 nm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated, and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Thr-389 phosphorylation of S6K was analyzed to show the inhibitory effects of rapamycin on mTORC1. Representative images from three independent experiments are shown. WCL, whole cell lysate.
Ros Scavengers Mntbap, supplied by Adipogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tmpyp4  (Merck KGaA)
90
Merck KGaA tmpyp4
<t>ROS-MK2</t> signaling is not involved in OA-induced Beclin 1 Ser-90 phosphorylation. A and B, HeLa cells were pretreated with or without ROS scavengers, <t>MnTBAP</t> (10 μm) and EUK134 (10 μm), for 30 min and treated with OA (100 nm) for 6 h. A, the level of ROS production was analyzed by a total ROS detection kit. BF, bright field. B, the level of Beclin 1 Ser-90 phosphorylation was analyzed by immunoblotting. Representative images from two and three independent experiments are shown. C, HeLa cells were treated with anisomycin (25 μg/ml) for 30 min or OA (100 nm) for 6 h. The levels of MK2 activation (shown by a band shift), Beclin 1 Ser-90 phosphorylation, and HSP27 Ser-82 phosphorylation were analyzed by immunoblotting. Representative images from three independent experiments are shown. D, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the AMPK activator 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) (1 mm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated (IP), and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Representative images from three independent experiments are shown. E, 293T cells were transfected with empty plasmid or FLAG-tagged constitutively active (CA) AMPK. Beclin 1 Ser-90 and raptor Ser-792 phosphorylation was analyzed by immunoblotting. Representative images from two independent experiments are shown. F, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the mTORC1 inhibitor rapamycin (100 nm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated, and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Thr-389 phosphorylation of S6K was analyzed to show the inhibitory effects of rapamycin on mTORC1. Representative images from three independent experiments are shown. WCL, whole cell lysate.
Tmpyp4, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mntbap  (AG Scientific)
90
AG Scientific mntbap
<t>ROS-MK2</t> signaling is not involved in OA-induced Beclin 1 Ser-90 phosphorylation. A and B, HeLa cells were pretreated with or without ROS scavengers, <t>MnTBAP</t> (10 μm) and EUK134 (10 μm), for 30 min and treated with OA (100 nm) for 6 h. A, the level of ROS production was analyzed by a total ROS detection kit. BF, bright field. B, the level of Beclin 1 Ser-90 phosphorylation was analyzed by immunoblotting. Representative images from two and three independent experiments are shown. C, HeLa cells were treated with anisomycin (25 μg/ml) for 30 min or OA (100 nm) for 6 h. The levels of MK2 activation (shown by a band shift), Beclin 1 Ser-90 phosphorylation, and HSP27 Ser-82 phosphorylation were analyzed by immunoblotting. Representative images from three independent experiments are shown. D, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the AMPK activator 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) (1 mm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated (IP), and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Representative images from three independent experiments are shown. E, 293T cells were transfected with empty plasmid or FLAG-tagged constitutively active (CA) AMPK. Beclin 1 Ser-90 and raptor Ser-792 phosphorylation was analyzed by immunoblotting. Representative images from two independent experiments are shown. F, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the mTORC1 inhibitor rapamycin (100 nm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated, and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Thr-389 phosphorylation of S6K was analyzed to show the inhibitory effects of rapamycin on mTORC1. Representative images from three independent experiments are shown. WCL, whole cell lysate.
Mntbap, supplied by AG Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mntbap  (Verlag GmbH)
90
Verlag GmbH mntbap
<t>ROS-MK2</t> signaling is not involved in OA-induced Beclin 1 Ser-90 phosphorylation. A and B, HeLa cells were pretreated with or without ROS scavengers, <t>MnTBAP</t> (10 μm) and EUK134 (10 μm), for 30 min and treated with OA (100 nm) for 6 h. A, the level of ROS production was analyzed by a total ROS detection kit. BF, bright field. B, the level of Beclin 1 Ser-90 phosphorylation was analyzed by immunoblotting. Representative images from two and three independent experiments are shown. C, HeLa cells were treated with anisomycin (25 μg/ml) for 30 min or OA (100 nm) for 6 h. The levels of MK2 activation (shown by a band shift), Beclin 1 Ser-90 phosphorylation, and HSP27 Ser-82 phosphorylation were analyzed by immunoblotting. Representative images from three independent experiments are shown. D, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the AMPK activator 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) (1 mm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated (IP), and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Representative images from three independent experiments are shown. E, 293T cells were transfected with empty plasmid or FLAG-tagged constitutively active (CA) AMPK. Beclin 1 Ser-90 and raptor Ser-792 phosphorylation was analyzed by immunoblotting. Representative images from two independent experiments are shown. F, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the mTORC1 inhibitor rapamycin (100 nm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated, and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Thr-389 phosphorylation of S6K was analyzed to show the inhibitory effects of rapamycin on mTORC1. Representative images from three independent experiments are shown. WCL, whole cell lysate.
Mntbap, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mntbap/product/Verlag GmbH
Average 90 stars, based on 1 article reviews
mntbap - by Bioz Stars, 2026-02
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ros/rns scavenger mntbap  (Merck KGaA)
90
Merck KGaA ros/rns scavenger mntbap
<t>ROS-MK2</t> signaling is not involved in OA-induced Beclin 1 Ser-90 phosphorylation. A and B, HeLa cells were pretreated with or without ROS scavengers, <t>MnTBAP</t> (10 μm) and EUK134 (10 μm), for 30 min and treated with OA (100 nm) for 6 h. A, the level of ROS production was analyzed by a total ROS detection kit. BF, bright field. B, the level of Beclin 1 Ser-90 phosphorylation was analyzed by immunoblotting. Representative images from two and three independent experiments are shown. C, HeLa cells were treated with anisomycin (25 μg/ml) for 30 min or OA (100 nm) for 6 h. The levels of MK2 activation (shown by a band shift), Beclin 1 Ser-90 phosphorylation, and HSP27 Ser-82 phosphorylation were analyzed by immunoblotting. Representative images from three independent experiments are shown. D, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the AMPK activator 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) (1 mm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated (IP), and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Representative images from three independent experiments are shown. E, 293T cells were transfected with empty plasmid or FLAG-tagged constitutively active (CA) AMPK. Beclin 1 Ser-90 and raptor Ser-792 phosphorylation was analyzed by immunoblotting. Representative images from two independent experiments are shown. F, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the mTORC1 inhibitor rapamycin (100 nm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated, and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Thr-389 phosphorylation of S6K was analyzed to show the inhibitory effects of rapamycin on mTORC1. Representative images from three independent experiments are shown. WCL, whole cell lysate.
Ros/Rns Scavenger Mntbap, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ros/rns scavenger mntbap/product/Merck KGaA
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90/100 stars
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mntbap  (Biomol GmbH)
90
Biomol GmbH mntbap
<t>ROS-MK2</t> signaling is not involved in OA-induced Beclin 1 Ser-90 phosphorylation. A and B, HeLa cells were pretreated with or without ROS scavengers, <t>MnTBAP</t> (10 μm) and EUK134 (10 μm), for 30 min and treated with OA (100 nm) for 6 h. A, the level of ROS production was analyzed by a total ROS detection kit. BF, bright field. B, the level of Beclin 1 Ser-90 phosphorylation was analyzed by immunoblotting. Representative images from two and three independent experiments are shown. C, HeLa cells were treated with anisomycin (25 μg/ml) for 30 min or OA (100 nm) for 6 h. The levels of MK2 activation (shown by a band shift), Beclin 1 Ser-90 phosphorylation, and HSP27 Ser-82 phosphorylation were analyzed by immunoblotting. Representative images from three independent experiments are shown. D, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the AMPK activator 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) (1 mm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated (IP), and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Representative images from three independent experiments are shown. E, 293T cells were transfected with empty plasmid or FLAG-tagged constitutively active (CA) AMPK. Beclin 1 Ser-90 and raptor Ser-792 phosphorylation was analyzed by immunoblotting. Representative images from two independent experiments are shown. F, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the mTORC1 inhibitor rapamycin (100 nm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated, and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Thr-389 phosphorylation of S6K was analyzed to show the inhibitory effects of rapamycin on mTORC1. Representative images from three independent experiments are shown. WCL, whole cell lysate.
Mntbap, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mntbap/product/Biomol GmbH
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superoxyde neutralization mntbap  (AG Scientific)
90
AG Scientific superoxyde neutralization mntbap
<t>ROS-MK2</t> signaling is not involved in OA-induced Beclin 1 Ser-90 phosphorylation. A and B, HeLa cells were pretreated with or without ROS scavengers, <t>MnTBAP</t> (10 μm) and EUK134 (10 μm), for 30 min and treated with OA (100 nm) for 6 h. A, the level of ROS production was analyzed by a total ROS detection kit. BF, bright field. B, the level of Beclin 1 Ser-90 phosphorylation was analyzed by immunoblotting. Representative images from two and three independent experiments are shown. C, HeLa cells were treated with anisomycin (25 μg/ml) for 30 min or OA (100 nm) for 6 h. The levels of MK2 activation (shown by a band shift), Beclin 1 Ser-90 phosphorylation, and HSP27 Ser-82 phosphorylation were analyzed by immunoblotting. Representative images from three independent experiments are shown. D, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the AMPK activator 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) (1 mm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated (IP), and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Representative images from three independent experiments are shown. E, 293T cells were transfected with empty plasmid or FLAG-tagged constitutively active (CA) AMPK. Beclin 1 Ser-90 and raptor Ser-792 phosphorylation was analyzed by immunoblotting. Representative images from two independent experiments are shown. F, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the mTORC1 inhibitor rapamycin (100 nm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated, and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Thr-389 phosphorylation of S6K was analyzed to show the inhibitory effects of rapamycin on mTORC1. Representative images from three independent experiments are shown. WCL, whole cell lysate.
Superoxyde Neutralization Mntbap, supplied by AG Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


ROS-MK2 signaling is not involved in OA-induced Beclin 1 Ser-90 phosphorylation. A and B, HeLa cells were pretreated with or without ROS scavengers, MnTBAP (10 μm) and EUK134 (10 μm), for 30 min and treated with OA (100 nm) for 6 h. A, the level of ROS production was analyzed by a total ROS detection kit. BF, bright field. B, the level of Beclin 1 Ser-90 phosphorylation was analyzed by immunoblotting. Representative images from two and three independent experiments are shown. C, HeLa cells were treated with anisomycin (25 μg/ml) for 30 min or OA (100 nm) for 6 h. The levels of MK2 activation (shown by a band shift), Beclin 1 Ser-90 phosphorylation, and HSP27 Ser-82 phosphorylation were analyzed by immunoblotting. Representative images from three independent experiments are shown. D, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the AMPK activator 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) (1 mm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated (IP), and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Representative images from three independent experiments are shown. E, 293T cells were transfected with empty plasmid or FLAG-tagged constitutively active (CA) AMPK. Beclin 1 Ser-90 and raptor Ser-792 phosphorylation was analyzed by immunoblotting. Representative images from two independent experiments are shown. F, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the mTORC1 inhibitor rapamycin (100 nm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated, and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Thr-389 phosphorylation of S6K was analyzed to show the inhibitory effects of rapamycin on mTORC1. Representative images from three independent experiments are shown. WCL, whole cell lysate.

Journal: The Journal of Biological Chemistry

Article Title: Regulation of Beclin 1 Protein Phosphorylation and Autophagy by Protein Phosphatase 2A (PP2A) and Death-associated Protein Kinase 3 (DAPK3) *

doi: 10.1074/jbc.M115.704908

Figure Lengend Snippet: ROS-MK2 signaling is not involved in OA-induced Beclin 1 Ser-90 phosphorylation. A and B, HeLa cells were pretreated with or without ROS scavengers, MnTBAP (10 μm) and EUK134 (10 μm), for 30 min and treated with OA (100 nm) for 6 h. A, the level of ROS production was analyzed by a total ROS detection kit. BF, bright field. B, the level of Beclin 1 Ser-90 phosphorylation was analyzed by immunoblotting. Representative images from two and three independent experiments are shown. C, HeLa cells were treated with anisomycin (25 μg/ml) for 30 min or OA (100 nm) for 6 h. The levels of MK2 activation (shown by a band shift), Beclin 1 Ser-90 phosphorylation, and HSP27 Ser-82 phosphorylation were analyzed by immunoblotting. Representative images from three independent experiments are shown. D, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the AMPK activator 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) (1 mm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated (IP), and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Representative images from three independent experiments are shown. E, 293T cells were transfected with empty plasmid or FLAG-tagged constitutively active (CA) AMPK. Beclin 1 Ser-90 and raptor Ser-792 phosphorylation was analyzed by immunoblotting. Representative images from two independent experiments are shown. F, MCF7 cells stably expressing FLAG-Beclin 1 WT were treated with the mTORC1 inhibitor rapamycin (100 nm) for the indicated time periods. FLAG-Beclin 1 was immunoprecipitated, and the phosphorylation of Beclin 1 Ser-90 was detected by immunoblotting. Thr-389 phosphorylation of S6K was analyzed to show the inhibitory effects of rapamycin on mTORC1. Representative images from three independent experiments are shown. WCL, whole cell lysate.

Article Snippet: Cells were pretreated with ROS scavengers, MnTBAP (AdipoGen) and EUK134 (Cayman), for 30 min and stimulated with OA for 6 h. ROS generation was detected using a total ROS detection kit for microscopy and flow cytometry (Enzo Life Sciences) according to the instructions of the manufacturer instruction.

Techniques: Phospho-proteomics, Western Blot, Activation Assay, Electrophoretic Mobility Shift Assay, Stable Transfection, Expressing, Immunoprecipitation, Transfection, Plasmid Preparation