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Image Search Results
Journal: ACS nano
Article Title: Echinacoside-Zinc Nanomaterial Inhibits Skin Glycation by Suppressing the Transcriptional Activation of the Receptor for Advanced Glycation End-Products.
doi: 10.1021/acsnano.3c04726
Figure Lengend Snippet: Figure 2. PPZn exerted anti-antiglycan in glycated model mouse skin. (A−C) Representative images of immunohistochemical (IHC) staining (A−a), HE staining (B−a), and Masson staining (C−a) of mouse skin tissue after the indicated treatment. The statistical results of the IHC staining index (A−b), epidermal thickness (B−b), and collagen density (C−b) are shown on the right. Scale bar, 50 μm. (D) qRT- PCR detection of RAGE mRNA levels in skin tissues of mice after the indicated treatment. (E) Western blot representative images and quantitative analysis of RAGE, COL1A2, MMP1, AGEs, and β-actin protein levels in skin tissues of mice after the indicated treatment. (F) Representative images of TUNEL staining of mouse skin tissue after the indicated treatment. Scale bar, 50 μm. (G−I) Relative Hyp content (G), SOD activity (H), and MDA concentration (I) in skin tissues of mice after the indicated treatment. All values are presented as the mean ± SD; P-values determined by two-sided Student’s t test. *P < 0.05, **P < 0.01, compared with the model.
Article Snippet: After being blocked, samples were then incubated with primary antibodies against MDM2 (Proteintech, 1:200), RAGE (Santa, 1:50), STAT2 (Zenbio, 1:100),
Techniques: Immunohistochemical staining, Immunohistochemistry, Staining, Quantitative RT-PCR, Western Blot, TUNEL Assay, Activity Assay, Concentration Assay
Journal: ACS nano
Article Title: Echinacoside-Zinc Nanomaterial Inhibits Skin Glycation by Suppressing the Transcriptional Activation of the Receptor for Advanced Glycation End-Products.
doi: 10.1021/acsnano.3c04726
Figure Lengend Snippet: Figure 3. PPZn exerted antiglycation effects in HaCaT cells. (A) Western blot representative images and quantitative analysis of RAGE, COL1A2, MMP1, AGEs, and β-actin protein levels in HaCaT cells after the indicated treatment. (B) Cell cycle was determined by flow cytometry in HaCaT cells after the indicated treatment. (C) Representative images of TUNEL staining of HaCaT cells after the indicated treatment. Scale bar, 50 μm. (D, E) Relative MDA concentration (D) and SOD activity (E) in HaCaT cells after the indicated treatment. All values are presented as the mean ± SD; P-values determined by two-sided Student’s t test. *P < 0.05, **P < 0.01, compared with the model.
Article Snippet: After being blocked, samples were then incubated with primary antibodies against MDM2 (Proteintech, 1:200), RAGE (Santa, 1:50), STAT2 (Zenbio, 1:100),
Techniques: Western Blot, Flow Cytometry, TUNEL Assay, Staining, Concentration Assay, Activity Assay
Journal: BMC Research Notes
Article Title: Multiplex preamplification of specific cDNA targets prior to gene expression analysis by TaqMan Arrays
doi: 10.1186/1756-0500-1-21
Figure Lengend Snippet: Mean CT and mean AE for the 46 bladder cancer related genes and the endogenous control GUSB obtained before and after cDNA preamplification.
Article Snippet: MMP1 ,
Techniques: Control