Journal: PLOS ONE

Article Title: Leveraging an advanced simulated moving bed approach to achieve 3-component separation for enhanced impurity removal in a non-affinity cation exchange capture step

doi: 10.1371/journal.pone.0280760

Figure Lengend Snippet: Process parameters of CEX, AEX, HIC and multimodal chromatography batch protocols. A residence time of 2 min was used for all steps unless otherwise stated.

Article Snippet: The 96-well plate screening of optimal conditions for polishing steps using POROS HQ (Thermo Fisher Scientific) anion exchange (AEX) resin, POROS Benzyl Ultra (Thermo Fisher Scientific) hydrophobic (HIC) resin and Capto MMC ImpRes (Cytiva) multimodal resin were performed sequentially at a 25 mg/mL-R load.

Techniques: Chromatography, Stripping Membranes

Journal: Toxins

Article Title: Co-Expression and Co-Purification Enable Manufacturing of a Six-Monoclonal Antibody Botulinum Antitoxin Cocktail

doi: 10.3390/toxins18050199

Figure Lengend Snippet: IEX HPLC Analysis of 5 mL HiTrap Capto S Column Fraction 15 (blue) and S column load (black). The inset shows the protein concentration measured by A280 on the Nanodrop spectrophotometer for the collected peak fractions (1 mL per fraction). Fraction 15 in the histogram is highlighted in blue. (IEX-HPLC of individual purified antibodies is shown in .) The column was loaded with 20 μg antibody.

Article Snippet: Cytiva HiTrap Capto MMC, Cytiva MabSelect PrismA, Cytiva Fibro PrismA, Eshmuno CP-FT, Cytiva Capto Q (1 mL), Cytiva Capto S (1 mL), Cytiva Capto Adhere (1 mL), MabSelect SuRe resin, MilliporeSigma ViResolve Modus 1.1 viral reduction filter (Sigma, St. Louis, MO, USA), Pall (Port Washington, NY, USA) Cadence Omega 0.1 m 2 30 kDa TFF cassette.

Techniques: Protein Concentration, Spectrophotometry, Purification