Journal: Sensors (Basel, Switzerland)

Article Title: Recent Advances in Rapid and Highly Sensitive Detection of Proteins and Specific DNA Sequences Using a Magnetic Modulation Biosensing System

doi: 10.3390/s22124497

Figure Lengend Snippet: Sensitivity, specificity, and cross-reactivity of the MMB Zika IgM and IgG serological assays. ( a ) Zika IgM and ( b ) Zika IgG assay results. The Controls (blue) were taken from healthy patients. The DENV (red) and WNV (black) are Dengue virus (DENV)- and West Nile virus (WNV)-positive samples were taken from enzyme-linked immunosorbent assay-positive patients. All samples were obtained from the National Center for Zoonotic Viruses at the Central Virology Laboratory of the Ministry of Health at Sheba Medical Center, Israel. The ZIKV (green) are Zika Virus positive samples taken from quantitative reverse-transcription polymerase chain reaction/neutralization-positive patients. The IgM and IgG samples were obtained on days 1–60 and 7–180 post-symptom onset, respectively. The Zika-positive samples were collected from Israeli travelers presenting at the Institute of Tropical Medicine at Sheba Medical Center after returning from Zika-endemic areas. The MMB IgM assay detected 23 of 26 Zika-positive samples (88% sensitivity), and the MMB IgG assay detected 33 of 34 Zika-positive samples (97% sensitivity) The specificity of the MMB assay for both IgM and IgG was 100%. The cross-reactivity to WNV was 0% for both IgM and IgG. The cross-reactivity to DENV was 0% for IgM and 4% for IgG. Reprinted from by permission of Oxford University Press 2018.

Article Snippet: For example, in a recent study, the MMB ZIKV serological assay detected as little as 7×10 3 and 9×10 3 ng/L of anti-ZIKV IgM and IgG antibodies, respectively, concentrations that are ~85- and ~5-fold lower than the concentrations detected by EUROIMMUN ELISA [ ].

Techniques: Virus, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Polymerase Chain Reaction, Neutralization

Journal: Sensors (Basel, Switzerland)

Article Title: Recent Advances in Rapid and Highly Sensitive Detection of Proteins and Specific DNA Sequences Using a Magnetic Modulation Biosensing System

doi: 10.3390/s22124497

Figure Lengend Snippet: Summary of different applications using the MMB and its advancements.

Article Snippet: For example, in a recent study, the MMB ZIKV serological assay detected as little as 7×10 3 and 9×10 3 ng/L of anti-ZIKV IgM and IgG antibodies, respectively, concentrations that are ~85- and ~5-fold lower than the concentrations detected by EUROIMMUN ELISA [ ].

Techniques: Serologic Assay, Inhibition, Translocation Assay, Sequencing, Biomarker Discovery