Journal: bioRxiv

Article Title: Resistance to Radiation Enhances Metastasis by Altering RNA Metabolism

doi: 10.1101/2025.02.19.638943

Figure Lengend Snippet: A. A Venn diagram showing the transcription factor genesets that were upregulated based on GSEA of the RNA-seq comparing the radioresistant cell lines to the parental cell lines along with the transcription factors that were identified by ENCODE transcription factor ChIP-seq data to regulate HNRNPL. B. A correlation between NRF2 activity score compared to HNRNPL expression in breast cancer patient samples collected by TCGA (n=1108, Spearman correlation). C. Immunofluorescent images of the MDA-MB-468-RR and MDA-MB-468 cells with blue being the DAPI dye and green representing NRF2. Scale bar is 10um. A ratio comparing NRF2 localization in the nucleus vs the cytoplasm for the MDA-MB-468-RR (n=52) vs MDA-MB-468 cells (n=47). Data represented as mean ± SD, and statistical analysis involved one-way ANOVA with Dunnett’s multiple comparisons tests. C. The mRNA expression of NRF2 and HNRNPL for the MDA-MB-468-RR and NRF2-knockdown cells (shNRF2-1 and shNRF2-2). n=3, mean ± SD, one-way ANOVA with Dunnett’s multiple comparisons tests. D. The mRNA expression of NRF2 and HNRNPL for the 4T1-RR and NRF2-knockdown cells (shNRF2-1 and shNRF2-2). n=3, mean ± SD, one-way ANOVA with Dunnett’s multiple comparisons tests. E. The mRNA expression of NRF2 and HNRNPL for the MDA-MB-468-RR cells treated with DMSO or ML385 (5uM) for 72 hours (n=3, mean ± SD, one-way ANOVA with Dunnett’s multiple comparisons tests). F. The mRNA expression of NRF2 and HNRNPL for the MDA-MB-468 cells treated with DMSO or DMF (5uM) for 24 hours (n=3, mean ± SD, unpaired t-test two tailed). G. The fold enrichment of NRF2 binding to the HNRNPL promoter region identified by ChIP-qPCR when pulling down NRF2 vs IgG (n=3, mean ± SD, unpaired t-test two tailed).

Article Snippet: The NRF2 inhibitor, ML385, was ordered from MedChemExpress (HY-100523); the NRF2 inducer, Dimethyl Fumarate was ordered from Sigma (242926).

Techniques: RNA Sequencing, ChIP-sequencing, Activity Assay, Expressing, Knockdown, Two Tailed Test, Binding Assay, ChIP-qPCR

Journal: Journal of Functional Foods

Article Title: Schisandra chinensis polysaccharide protects against cyclosporin A-induced liver injury by promoting hepatocyte proliferation

doi: 10.1016/j.jff.2021.104799

Figure Lengend Snippet: Fig. 7. SCP activated Nrf2 in LX-2 cells induced by CsA. (A). LX-2 cells were treated with SCP (250 μg/mL), CsA (15 μM for 48 h; nuclear proteins of Nrf2 was detected by using western blotting. (B). LX-2 cells were treated with SCP (250 μg/mL), CsA (15 μM, and ML385 (25 μM) for 48 h, nuclear proteins of Nrf2 and total priteins of BDNF were detected by using western blotting (WB). (C-D). LX-2 and LX-2 mKate2 Cells proliferation was detected by Real-Time Live-Cell Imaging System. Results were means ± SD, #P < 0.05 and ## P < 0.01 vs. Normal group; *P < 0.05, **P < 0.01, and ***P < 0.001 vs. CsA group.

Article Snippet: ML385 was purchased from Selleck Chemicals LLC (Houston, TX, USA).

Techniques: Western Blot, Live Cell Imaging

Journal: Frontiers in Pharmacology

Article Title: Astragaloside IV attenuates ferroptosis after subarachnoid hemorrhage via Nrf2/HO-1 signaling pathway

doi: 10.3389/fphar.2022.924826

Figure Lengend Snippet: Animal usage and mortality.

Article Snippet: ML385 (50 pmol/5 μl; AOBIOUS, Gloucester, MA, United States), a specific Nrf2 inhibitor, was diluted in DMSO, and was used for intracerebroventricular (i.c.v.) injection at 24 h before SAH.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Astragaloside IV attenuates ferroptosis after subarachnoid hemorrhage via Nrf2/HO-1 signaling pathway

doi: 10.3389/fphar.2022.924826

Figure Lengend Snippet: ML385 inhibits the neuroprotective effect of AS-IV at 24 h post-SAH. (A–C) Modified Garcia scores, beam balance scores, and brain water content 24 h after SAH in rats ( n = 8). (D,E) Representative images and quantitative analysis of FJB staining in right temporal cerebral cortex of rats (n = 6, scale bar = 50 μm). (F) Quantification of iron concentrations in rats after SAH ( n = 8). (G–I) Quantitative analysis of lipid ROS, MDA, and GSH levels ( n = 8). (J) Representative Western blot images of SLC7A11 and GPX4. (K) Quantitative analysis of SLC7A11 and GPX4 ( n = 6). Data are represented as mean ± SD. * p < 0.05, NS: not statistically significant; DMSO: dimethyl sulfoxide; FJB, Fluoro-Jade B .

Article Snippet: ML385 (50 pmol/5 μl; AOBIOUS, Gloucester, MA, United States), a specific Nrf2 inhibitor, was diluted in DMSO, and was used for intracerebroventricular (i.c.v.) injection at 24 h before SAH.

Techniques: Modification, Staining, Western Blot