ml265 Search Results


pyruvate kinase m2 pkm2 activator ml265  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology pyruvate kinase m2 pkm2 activator ml265
Pyruvate Kinase M2 Pkm2 Activator Ml265, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ml265/ML+265/pm30718913-411-13-32
Average 90 stars, based on 1 article reviews
pyruvate kinase m2 pkm2 activator ml265 - by Bioz Stars, 2026-10
90/100 stars
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tepp  (MedChemExpress)
96
MedChemExpress tepp
FIGURE 5 Loss of ATG5 reduces PKM2 autophagy degradation. A, Eight-week-old male WT and SIRT3-/- mice were given 1400 ng/kg/ min of Ang II or saline for 14 days. Representative fluorescence micrographs of en face staining with CD31 (green) and PKM2 (red) in the aortic endothelia. Scale bar: 100 μm. B, Fluorescence intensity of PKM2 (n = 7). C, Representative native western blots of PKM2 in MAECs infected with shRNA-SIRT3, LV-SIRT3, or empty vector. D, Quantitative analysis of PKM2 dimer expression (n = 3). E, Representative native western blots of PKM2 in MAECs infected with shRNA-ATG5, LV-ATG5, or empty vector after 24 hours Ang II treatment. F, Quantitative analysis of PKM2 dimer expression (n = 3). G, Lactate in culture media from MAECs infected with shRNA-SIRT3, LV-SIRT3, shRNA-ATG5, LV-ATG5, or empty vector (n = 6). H, MAECs were preincubated <t>with</t> <t>TEPP-46</t> (2 x 10-4 mol/L) for 1 hour, followed by Ang II treatment for 24 hours. Representative western blots of PKM2, CD31, and α-SMA in MAECs. I, Quantitative analysis of α-SMA and CD31 (n = 6). J, Lactate in culture media from MAECs treated with Ang II combined with or without TEPP46 (n = 6). K, Eight-week-old male mice were given 1400 ng/kg/min of Ang II or saline for 14 days. TEPP-46 was intraperitoneal injection 3 d prior to Ang II or saline infusion. Representative fluorescence micrographs of en face staining with CD31 and α-SMA in the aortic endothelia. L, Fluorescence intensity of α-SMA (n = 7). *P < .05, **P < .01 vs control. #P < .05, ##P < 0.01 vs Ang II treatment group
Tepp, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ml265/TEPP-46/10__1096_slash_fj__202001494r-34-0-4
Average 96 stars, based on 1 article reviews
tepp - by Bioz Stars, 2026-10
96/100 stars
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ml265  (ApexBio)
90
ApexBio ml265
FIGURE 5 Loss of ATG5 reduces PKM2 autophagy degradation. A, Eight-week-old male WT and SIRT3-/- mice were given 1400 ng/kg/ min of Ang II or saline for 14 days. Representative fluorescence micrographs of en face staining with CD31 (green) and PKM2 (red) in the aortic endothelia. Scale bar: 100 μm. B, Fluorescence intensity of PKM2 (n = 7). C, Representative native western blots of PKM2 in MAECs infected with shRNA-SIRT3, LV-SIRT3, or empty vector. D, Quantitative analysis of PKM2 dimer expression (n = 3). E, Representative native western blots of PKM2 in MAECs infected with shRNA-ATG5, LV-ATG5, or empty vector after 24 hours Ang II treatment. F, Quantitative analysis of PKM2 dimer expression (n = 3). G, Lactate in culture media from MAECs infected with shRNA-SIRT3, LV-SIRT3, shRNA-ATG5, LV-ATG5, or empty vector (n = 6). H, MAECs were preincubated <t>with</t> <t>TEPP-46</t> (2 x 10-4 mol/L) for 1 hour, followed by Ang II treatment for 24 hours. Representative western blots of PKM2, CD31, and α-SMA in MAECs. I, Quantitative analysis of α-SMA and CD31 (n = 6). J, Lactate in culture media from MAECs treated with Ang II combined with or without TEPP46 (n = 6). K, Eight-week-old male mice were given 1400 ng/kg/min of Ang II or saline for 14 days. TEPP-46 was intraperitoneal injection 3 d prior to Ang II or saline infusion. Representative fluorescence micrographs of en face staining with CD31 and α-SMA in the aortic endothelia. L, Fluorescence intensity of α-SMA (n = 7). *P < .05, **P < .01 vs control. #P < .05, ##P < 0.01 vs Ang II treatment group
Ml265, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ml265/ml265/pm37399615-150-0-6
Average 90 stars, based on 1 article reviews
ml265 - by Bioz Stars, 2026-10
90/100 stars
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ml 265  (Cayman Chemical)
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ML 265
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ml 265  (Aladdin Scientific Corporation)
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tepp-46 (ml265)  (Aladdin Scientific Corporation)
N/A
InformationTEPP-46 (ML265) TEPP-46 (ML265, CID-44246499, NCGC00186528) is a potent activator of PKM2 in both biochemical (AC50 = 92 nM) and cell-based assays with high selectivity over PKM1, PKR and PKL.In vitroML265 potently activates PKM2 in
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Image Search Results


FIGURE 5 Loss of ATG5 reduces PKM2 autophagy degradation. A, Eight-week-old male WT and SIRT3-/- mice were given 1400 ng/kg/ min of Ang II or saline for 14 days. Representative fluorescence micrographs of en face staining with CD31 (green) and PKM2 (red) in the aortic endothelia. Scale bar: 100 μm. B, Fluorescence intensity of PKM2 (n = 7). C, Representative native western blots of PKM2 in MAECs infected with shRNA-SIRT3, LV-SIRT3, or empty vector. D, Quantitative analysis of PKM2 dimer expression (n = 3). E, Representative native western blots of PKM2 in MAECs infected with shRNA-ATG5, LV-ATG5, or empty vector after 24 hours Ang II treatment. F, Quantitative analysis of PKM2 dimer expression (n = 3). G, Lactate in culture media from MAECs infected with shRNA-SIRT3, LV-SIRT3, shRNA-ATG5, LV-ATG5, or empty vector (n = 6). H, MAECs were preincubated with TEPP-46 (2 x 10-4 mol/L) for 1 hour, followed by Ang II treatment for 24 hours. Representative western blots of PKM2, CD31, and α-SMA in MAECs. I, Quantitative analysis of α-SMA and CD31 (n = 6). J, Lactate in culture media from MAECs treated with Ang II combined with or without TEPP46 (n = 6). K, Eight-week-old male mice were given 1400 ng/kg/min of Ang II or saline for 14 days. TEPP-46 was intraperitoneal injection 3 d prior to Ang II or saline infusion. Representative fluorescence micrographs of en face staining with CD31 and α-SMA in the aortic endothelia. L, Fluorescence intensity of α-SMA (n = 7). *P < .05, **P < .01 vs control. #P < .05, ##P < 0.01 vs Ang II treatment group

Journal: The FASEB Journal

Article Title: Sirtuin 3 governs autophagy‐dependent glycolysis during Angiotensin II‐induced endothelial‐to‐mesenchymal transition

doi: 10.1096/fj.202001494r

Figure Lengend Snippet: FIGURE 5 Loss of ATG5 reduces PKM2 autophagy degradation. A, Eight-week-old male WT and SIRT3-/- mice were given 1400 ng/kg/ min of Ang II or saline for 14 days. Representative fluorescence micrographs of en face staining with CD31 (green) and PKM2 (red) in the aortic endothelia. Scale bar: 100 μm. B, Fluorescence intensity of PKM2 (n = 7). C, Representative native western blots of PKM2 in MAECs infected with shRNA-SIRT3, LV-SIRT3, or empty vector. D, Quantitative analysis of PKM2 dimer expression (n = 3). E, Representative native western blots of PKM2 in MAECs infected with shRNA-ATG5, LV-ATG5, or empty vector after 24 hours Ang II treatment. F, Quantitative analysis of PKM2 dimer expression (n = 3). G, Lactate in culture media from MAECs infected with shRNA-SIRT3, LV-SIRT3, shRNA-ATG5, LV-ATG5, or empty vector (n = 6). H, MAECs were preincubated with TEPP-46 (2 x 10-4 mol/L) for 1 hour, followed by Ang II treatment for 24 hours. Representative western blots of PKM2, CD31, and α-SMA in MAECs. I, Quantitative analysis of α-SMA and CD31 (n = 6). J, Lactate in culture media from MAECs treated with Ang II combined with or without TEPP46 (n = 6). K, Eight-week-old male mice were given 1400 ng/kg/min of Ang II or saline for 14 days. TEPP-46 was intraperitoneal injection 3 d prior to Ang II or saline infusion. Representative fluorescence micrographs of en face staining with CD31 and α-SMA in the aortic endothelia. L, Fluorescence intensity of α-SMA (n = 7). *P < .05, **P < .01 vs control. #P < .05, ##P < 0.01 vs Ang II treatment group

Article Snippet: TEPP-46 was purchased from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Saline, Fluorescence, Staining, Western Blot, Infection, shRNA, Plasmid Preparation, Expressing, Injection, Control