mkn 45 Search Results


mkn45  (CLS Cell Lines Service GmbH)
93
CLS Cell Lines Service GmbH mkn45
Mkn45, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
mkn45 - by Bioz Stars, 2026-05
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mkn45  (DSMZ)
95
DSMZ mkn45
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn45, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
mkn45 - by Bioz Stars, 2026-05
95/100 stars
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mkn 45  (AcceGen Biotechnology)
93
AcceGen Biotechnology mkn 45
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45, supplied by AcceGen Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mkn 45/product/AcceGen Biotechnology
Average 93 stars, based on 1 article reviews
mkn 45 - by Bioz Stars, 2026-05
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mkn 45  (Procell Inc)
86
Procell Inc mkn 45
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
mkn 45 - by Bioz Stars, 2026-05
86/100 stars
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human gastric cancer mkn-45 cell line  (Egens Biotechnology)
90
Egens Biotechnology human gastric cancer mkn-45 cell line
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Human Gastric Cancer Mkn 45 Cell Line, supplied by Egens Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human gastric cancer mkn-45 cell line/product/Egens Biotechnology
Average 90 stars, based on 1 article reviews
human gastric cancer mkn-45 cell line - by Bioz Stars, 2026-05
90/100 stars
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mkn-45 gc cells  (Biopharm GmbH)
90
Biopharm GmbH mkn-45 gc cells
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45 Gc Cells, supplied by Biopharm GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mkn-45 gc cells/product/Biopharm GmbH
Average 90 stars, based on 1 article reviews
mkn-45 gc cells - by Bioz Stars, 2026-05
90/100 stars
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human gc cells mkn-45  (Beijing Xiehe Pharmaceutical Co Ltd)
90
Beijing Xiehe Pharmaceutical Co Ltd human gc cells mkn-45
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Human Gc Cells Mkn 45, supplied by Beijing Xiehe Pharmaceutical Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human gc cells mkn-45/product/Beijing Xiehe Pharmaceutical Co Ltd
Average 90 stars, based on 1 article reviews
human gc cells mkn-45 - by Bioz Stars, 2026-05
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lentivirus transfected mkn-45 cells (shcpne1 or shnc)  (Shanghai GenePharma)
90
Shanghai GenePharma lentivirus transfected mkn-45 cells (shcpne1 or shnc)
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Lentivirus Transfected Mkn 45 Cells (Shcpne1 Or Shnc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lentivirus transfected mkn-45 cells (shcpne1 or shnc)/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
lentivirus transfected mkn-45 cells (shcpne1 or shnc) - by Bioz Stars, 2026-05
90/100 stars
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mkn-45/smac cells  (SMAC Corp)
90
SMAC Corp mkn-45/smac cells
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45/Smac Cells, supplied by SMAC Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mkn-45/smac cells/product/SMAC Corp
Average 90 stars, based on 1 article reviews
mkn-45/smac cells - by Bioz Stars, 2026-05
90/100 stars
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mkn-45 cell line  (Chemon Inc)
90
Chemon Inc mkn-45 cell line
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45 Cell Line, supplied by Chemon Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mkn-45 cell line/product/Chemon Inc
Average 90 stars, based on 1 article reviews
mkn-45 cell line - by Bioz Stars, 2026-05
90/100 stars
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mkn-45  (Technical Manufacturing Company)
90
Technical Manufacturing Company mkn-45
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45, supplied by Technical Manufacturing Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mkn-45/product/Technical Manufacturing Company
Average 90 stars, based on 1 article reviews
mkn-45 - by Bioz Stars, 2026-05
90/100 stars
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mkn-45 gc cells  (Allogen Laboratories)
90
Allogen Laboratories mkn-45 gc cells
Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, <t>MKN45,</t> St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM
Mkn 45 Gc Cells, supplied by Allogen Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mkn-45 gc cells/product/Allogen Laboratories
Average 90 stars, based on 1 article reviews
mkn-45 gc cells - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, MKN45, St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM

Journal: BMC Cancer

Article Title: The Gb3-synthase A4GALT is an epigenetically regulated driver of tumor invasiveness in gastrointestinal cancer

doi: 10.1186/s12885-026-15600-7

Figure Lengend Snippet: Gb 3 biosynthesis is correlated with A4GALT expression. a-c FACS analysis of Gb 3 surface levels and rt‑qPCR of A4GALT and α‑GLA in gastric (AGS, MKN45, St3051) and colorectal (DLD1, HCT116) cancer cell lines. Gb 3 content and A4GALT expression were undetectable in DLD1 and AGS cells, but high in St3051 and HCT116. GLA was expressed in all cell lines at varying levels (presented with mean ± SD; n ≥ 3). d Linear regression of the A4GALT/α‑GLA expression ratio vs. Gb 3 surface levels across all cell lines revealed a strong positive correlation ( p = 0.0016, R 2 = 0.98). The dashed magenta line indicates the regression fit and dotted grey lines the 95% confidence interval. e , f A4GALT and α‑GLA expression in Gb 3 ⁺ and Gb 3 ⁻ colorectal cancer tissues (classified by STxB staining, n = 17 patients). A4GALT expression was significantly higher in Gb 3 ⁺ samples ( p = 0.049), whereas α‑GLA expression did not differ significantly (Tukey box plot). g Gb 3 staining by immunofluorescence microscopy on colon cancer tissue sections. STxB-Cy3 (red) for Gb 3 , anti-vonWillebrand factor antibody for blood vessels (green), and nuclei (DAPI, blue), overlayed with phase contrast images. Two Gb 3 ⁺ cases are shown in the upper row, and two Gb 3 ⁻ cases in the lower row. As control, normal colon tissue was stained, showing essentially no Gb 3 detection (upper row, right panel). Control incubation: tumor tissue with BSA-Cy3 instead of STxB-Cy3, with DAPI and anti-vonWillebrand factor (lower row, right panel). Size bars: 25 µM

Article Snippet: The cell lines used in this study were the gastric cancer lines St3051 [ ], AGS (ATCC, CRL-1739), and MKN45 (DSMZ, ACC 409), and the colorectal cancer lines HCT116 (ATCC, CCL-247), DLD1 (ATCC, CCL-221).

Techniques: Expressing, Staining, Immunofluorescence, Microscopy, Control, Incubation