mki67 expression Search Results


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  • ki 67  (Abcam)
    88
    Abcam ki 67
    Ki 67, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ki 67/product/Abcam
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ki 67 - by Bioz Stars, 2022-09
    88/100 stars
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    88
    Cell Signaling Technology Inc ki 67
    Ki 67, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ki 67/product/Cell Signaling Technology Inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ki 67 - by Bioz Stars, 2022-09
    88/100 stars
      Buy from Supplier

    88
    Abcam antibodies ki 67
    Expression of proliferation and angiogenic markers. (A) Immunohistochemistry (IHC) microscopic analysis at 40X magnification showing the expression of proliferation <t>(Ki-67)</t> and angiogenic (CD31) markers in formalin- fixed, paraffin-embedded peritoneal tissue and diaphragm of mice from control and treatment groups euthanized on day 35. (B) Semiquantitative analysis of diaminobenzidine stained positive area from the IHC images for Ki-67 and CD31 expression using NIH Image J software.
    Antibodies Ki 67, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies ki 67/product/Abcam
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies ki 67 - by Bioz Stars, 2022-09
    88/100 stars
      Buy from Supplier

    80
    Abcam anti ki67 antibody
    Identification of the hiPSC. (A) Representative images displayed the typical morphology of hiPSC. (B) qRT-PCR results showed hiPSC has similar mRNA levels to the human embryonic stem cells (hESC) regarding the pluripotency marker genes (OCT4, SOX2, NANOG and KLF4). (C) Pluripotency markers of hiPSC (OCT4, NANOG, SSEA4 and TRA-1-60) were confirmed by immunofluorescence (IF) assay. (D) qRT-PCR results showed that hiPSC has similar mRNA levels to the hESC regarding the proliferation marker genes, <t>MKI67</t> and AURKB. (E) MKI67 was further identified using IF method. Expression values of the PCR analysis were normalized to GAPDH. Data are presented as mean±SD (t test, NS not significant; n=3).
    Anti Ki67 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ki67 antibody/product/Abcam
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ki67 antibody - by Bioz Stars, 2022-09
    80/100 stars
      Buy from Supplier

    Image Search Results


    Expression of proliferation and angiogenic markers. (A) Immunohistochemistry (IHC) microscopic analysis at 40X magnification showing the expression of proliferation (Ki-67) and angiogenic (CD31) markers in formalin- fixed, paraffin-embedded peritoneal tissue and diaphragm of mice from control and treatment groups euthanized on day 35. (B) Semiquantitative analysis of diaminobenzidine stained positive area from the IHC images for Ki-67 and CD31 expression using NIH Image J software.

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Enhanced Anti-tumor Efficacy and Safety with Metronomic Intraperitoneal Chemotherapy for Metastatic Ovarian Cancer using Biodegradable Nanotextile Implants

    doi: 10.1016/j.jconrel.2019.05.022

    Figure Lengend Snippet: Expression of proliferation and angiogenic markers. (A) Immunohistochemistry (IHC) microscopic analysis at 40X magnification showing the expression of proliferation (Ki-67) and angiogenic (CD31) markers in formalin- fixed, paraffin-embedded peritoneal tissue and diaphragm of mice from control and treatment groups euthanized on day 35. (B) Semiquantitative analysis of diaminobenzidine stained positive area from the IHC images for Ki-67 and CD31 expression using NIH Image J software.

    Article Snippet: Antibodies Ki-67 and CD31 for Immunohistochemistry (IHC) analysis were procured from Abcam (Cambridge, MA, USA) and BioGenex (Fremont, CA, USA).

    Techniques: Expressing, Immunohistochemistry, Formalin-fixed Paraffin-Embedded, Mouse Assay, Staining, Software

    Identification of the hiPSC. (A) Representative images displayed the typical morphology of hiPSC. (B) qRT-PCR results showed hiPSC has similar mRNA levels to the human embryonic stem cells (hESC) regarding the pluripotency marker genes (OCT4, SOX2, NANOG and KLF4). (C) Pluripotency markers of hiPSC (OCT4, NANOG, SSEA4 and TRA-1-60) were confirmed by immunofluorescence (IF) assay. (D) qRT-PCR results showed that hiPSC has similar mRNA levels to the hESC regarding the proliferation marker genes, MKI67 and AURKB. (E) MKI67 was further identified using IF method. Expression values of the PCR analysis were normalized to GAPDH. Data are presented as mean±SD (t test, NS not significant; n=3).

    Journal: International Journal of Stem Cells

    Article Title: p53 Promotes Differentiation of Cardiomyocytes from hiPSC through Wnt Signaling-Mediated Mesendodermal Differentiation

    doi: 10.15283/ijsc21051

    Figure Lengend Snippet: Identification of the hiPSC. (A) Representative images displayed the typical morphology of hiPSC. (B) qRT-PCR results showed hiPSC has similar mRNA levels to the human embryonic stem cells (hESC) regarding the pluripotency marker genes (OCT4, SOX2, NANOG and KLF4). (C) Pluripotency markers of hiPSC (OCT4, NANOG, SSEA4 and TRA-1-60) were confirmed by immunofluorescence (IF) assay. (D) qRT-PCR results showed that hiPSC has similar mRNA levels to the hESC regarding the proliferation marker genes, MKI67 and AURKB. (E) MKI67 was further identified using IF method. Expression values of the PCR analysis were normalized to GAPDH. Data are presented as mean±SD (t test, NS not significant; n=3).

    Article Snippet: 48 h later cells were fixed with 4% (w/v) Paraformaldehyde (PFA) for 15 min at room temperature, permeabilized, and incubated with following first primary antibodies: anti-OCT4 antibody (#2750, Cell Signaling Technology, USA), anti-NANOG antibody (#3580, Cell Signaling Technology, USA), anti-SSEA4 antibody (#4755, Cell Signaling Technology, USA), anti-TRA-1-60 antibody (#4746, Cell Signaling Technology, USA), anti-MKI67 antibody (ab15580, abcam, USA), anti-NKX2-5 antibody (ab91196, abcam, USA), anti-cTNT antibody (MS-295-P1, Thermo Fisher Scientific, USA) and anti-α-ACTININ antibody (A7811, Sigma, USA), followed by their corresponding fluorescence-labeled secondary antibodies, alexa fluor 488 labeled goat anti-rabbit IgG (A-11008, Invitrogen, USA), alexa fluor 488 labeled goat anti-mouse IgG (A-11001, Invitrogen, USA), alexa fluor 594 labeled goat anti-rabbit IgG (R37177, Invitrogen, USA) and alexa fluor 594 labeled goat anti-mouse IgG (A-11005, Invitrogen, USA).

    Techniques: Quantitative RT-PCR, Marker, Immunofluorescence, Expressing, Polymerase Chain Reaction

    Overexpression of P53 gene in hiPSC by lentivirus methods. (A) Representative images of GFP fluorescence displayed highly efficient infection of hiPSC cells in both P53 and control group. (B) The qRT-PCR results showed that hiPSC with lenti-P53 caused about 8-fold increase of P53 mRNA compared with control group. (C) Representative image of Western Blot showed significantly higher p53 protein level of lenti-P53 group than control, and quantification was done by densitometry (D). (E) The qRT-PCR results showed that overexpressed P53 had no significant effects on the mRNA expression of the pluripotency marker (OCT4, SOX2, NANOG and KLF4) of hiPSC. (F, G) The Western Blot results showed that overexpressed P53 did not change OCT4 protein level but slightly decreased Nanog with statistical significance. Representative image was shown (F) and quantified by densitometry (G). (H) hiPSC with lenti-P53 displayed similar mRNA levels of proliferation markers (MKI67 and AURKB). (I, J) Representative image of cell cycle analysis using flow cytometry showed cell cycle was not influenced by lenti-P53 (I) and quantification by densitometry (J). Expression values of the PCR analysis were normalized to GAPDH. Data are presented as mean±SD (t test, *p

    Journal: International Journal of Stem Cells

    Article Title: p53 Promotes Differentiation of Cardiomyocytes from hiPSC through Wnt Signaling-Mediated Mesendodermal Differentiation

    doi: 10.15283/ijsc21051

    Figure Lengend Snippet: Overexpression of P53 gene in hiPSC by lentivirus methods. (A) Representative images of GFP fluorescence displayed highly efficient infection of hiPSC cells in both P53 and control group. (B) The qRT-PCR results showed that hiPSC with lenti-P53 caused about 8-fold increase of P53 mRNA compared with control group. (C) Representative image of Western Blot showed significantly higher p53 protein level of lenti-P53 group than control, and quantification was done by densitometry (D). (E) The qRT-PCR results showed that overexpressed P53 had no significant effects on the mRNA expression of the pluripotency marker (OCT4, SOX2, NANOG and KLF4) of hiPSC. (F, G) The Western Blot results showed that overexpressed P53 did not change OCT4 protein level but slightly decreased Nanog with statistical significance. Representative image was shown (F) and quantified by densitometry (G). (H) hiPSC with lenti-P53 displayed similar mRNA levels of proliferation markers (MKI67 and AURKB). (I, J) Representative image of cell cycle analysis using flow cytometry showed cell cycle was not influenced by lenti-P53 (I) and quantification by densitometry (J). Expression values of the PCR analysis were normalized to GAPDH. Data are presented as mean±SD (t test, *p

    Article Snippet: 48 h later cells were fixed with 4% (w/v) Paraformaldehyde (PFA) for 15 min at room temperature, permeabilized, and incubated with following first primary antibodies: anti-OCT4 antibody (#2750, Cell Signaling Technology, USA), anti-NANOG antibody (#3580, Cell Signaling Technology, USA), anti-SSEA4 antibody (#4755, Cell Signaling Technology, USA), anti-TRA-1-60 antibody (#4746, Cell Signaling Technology, USA), anti-MKI67 antibody (ab15580, abcam, USA), anti-NKX2-5 antibody (ab91196, abcam, USA), anti-cTNT antibody (MS-295-P1, Thermo Fisher Scientific, USA) and anti-α-ACTININ antibody (A7811, Sigma, USA), followed by their corresponding fluorescence-labeled secondary antibodies, alexa fluor 488 labeled goat anti-rabbit IgG (A-11008, Invitrogen, USA), alexa fluor 488 labeled goat anti-mouse IgG (A-11001, Invitrogen, USA), alexa fluor 594 labeled goat anti-rabbit IgG (R37177, Invitrogen, USA) and alexa fluor 594 labeled goat anti-mouse IgG (A-11005, Invitrogen, USA).

    Techniques: Over Expression, Fluorescence, Infection, Quantitative RT-PCR, Western Blot, Expressing, Marker, Cell Cycle Assay, Flow Cytometry, Polymerase Chain Reaction