Journal: Nucleic Acids Research

Article Title: SETDB1 activity is globally directed by H3K14 acetylation via its Triple Tudor Domain

doi: 10.1093/nar/gkae1053

Figure Lengend Snippet: SETDB1 is recruited to H3K14ac containing target loci. ( A ) Scheme of the cell HCT116 derived lines generated in this study by SETDB1 KO followed by reconstitution with SETDB1 WT or mutants and by HBO1 KO. ( B and C ) H3K9me3 (B) and H3K14ac (C) ChIP-qPCR at known SETDB1 target regions ( , ) showing changes in the histone modification as indicated upon SETDB1 KO and reconstitution with WT SETDB1 of its catalytically inactive mutant H1224K or the 3TD mutant F332A. Moreover, HBO1 KO cells were investigated. ChIP was performed on mononucleosomes isolated from two individual biological replicates from each cell line (represented as dots). Averages of the individual measurements are represented as bars.

Article Snippet: For each region of interest, a master mix was prepared with 7.5 μl of 2X ORATM See qPCR Probe Mix (highQu), 0.4 μl forward primer, 0.4 μl reverse primer and 5.7 μl ddH 2 O.

Techniques: Derivative Assay, Generated, ChIP-qPCR, Modification, Mutagenesis, Isolation