Journal: Scientific Reports
Article Title: Pannexin1 links lymphatic function to lipid metabolism and atherosclerosis
Figure Lengend Snippet: Reduced atherosclerotic plaque stability in mice lacking Panx1. Representative images and quantification of (immuno-)stainings for CD68 ( A , E; brown signal), necrotic core ( B , F; yellow-marked acellular areas), collagen ( C , G; red signal) and α-SMA ( D , H; green signal) performed on aortic roots of Apoe −/− (white bars) and Panx1 −/− Apoe −/− (grey bars) mice after 5 weeks of HCD (n = 10). MCP-1-induced chemotaxis ( I ) and adhesion onto collagen-coated ( J ) or fibronectin-coated ( K ) surfaces of Panx1 fl/fl Apoe −/− (white bars) and Panx1 del Apoe −/− (black bars) BMDMs (n = 6). Number of apoptotic cells ( L ) using TUNEL staining or proliferating cells ( M ) using PCNA staining performed on aortic roots of Apoe −/− (white bars) and Panx1 −/− Apoe −/− (grey bars) mice after 5 weeks of HCD (n = 10). Scale bars represent 200 μm for A , or 100 μm for B , C , and D .
Article Snippet: For atherosclerosis studies, we used 10 weeks-old male Panx1 −/− Apoe −/− , Apoe −/− , Tie2Cre Tg Panx1 fl/fl Apoe −/− (Panx1 del Apoe −/− ) and Panx1 fl/fl Apoe −/− mice, on a C57BL/6 background, fed with HCD (1.25% cholesterol, 0% cholate; Research Diets Inc.) for 5 or 10 weeks.
Techniques: Mouse Assay, Chemotaxis Assay, TUNEL Assay, Staining