mito dsred2 Search Results


mitodsred  (Addgene inc)
93
Addgene inc mitodsred
Mitochondria occupy fine astrocyte processes concurrently with astrocyte morphological arborization in vitro . (A) Schematic of astrocyte–neuron co-culture assay. (B) Representative images of rat astrocytes transfected with EGFP (green, top panels) and <t>MitoDsRed</t> (magenta, middle panels) from 4 to 48 h in co-culture with cortical neurons. Inset (bottom panels) of distal astrocyte processes (green) housing mitochondria (magenta) at the leading edge of growing processes (white arrowheads) from 4 to 48 h in culture. Scale bars: 20 μm. (C) Overview of the Seg_Astro image analysis pipeline. (D) Astrocyte secondary, fine/distal, and terminal branch number at 4 vs. 48 h in culture. n = 10–12 cells per time point from one experiment. Unpaired two-tailed t test. (E and F) Total astrocyte mitochondria number and (F) average mitochondrial size in secondary, fine/distal, and terminal astrocyte branches from 4 to 48 h in culture. n = 10–12 cells per time point from one experiment. Data are presented as mean ± SEM. One-way ANOVA with Tukey post hoc test.
Mitodsred, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mito+dsred2/pmc12406776-268-0-4?v=Addgene+inc
Average 93 stars, based on 1 article reviews
mitodsred - by Bioz Stars, 2026-08
93/100 stars
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dsred2-mito-7  (MatTek)
90
MatTek dsred2-mito-7
Mitochondria occupy fine astrocyte processes concurrently with astrocyte morphological arborization in vitro . (A) Schematic of astrocyte–neuron co-culture assay. (B) Representative images of rat astrocytes transfected with EGFP (green, top panels) and <t>MitoDsRed</t> (magenta, middle panels) from 4 to 48 h in co-culture with cortical neurons. Inset (bottom panels) of distal astrocyte processes (green) housing mitochondria (magenta) at the leading edge of growing processes (white arrowheads) from 4 to 48 h in culture. Scale bars: 20 μm. (C) Overview of the Seg_Astro image analysis pipeline. (D) Astrocyte secondary, fine/distal, and terminal branch number at 4 vs. 48 h in culture. n = 10–12 cells per time point from one experiment. Unpaired two-tailed t test. (E and F) Total astrocyte mitochondria number and (F) average mitochondrial size in secondary, fine/distal, and terminal astrocyte branches from 4 to 48 h in culture. n = 10–12 cells per time point from one experiment. Data are presented as mean ± SEM. One-way ANOVA with Tukey post hoc test.
Dsred2 Mito 7, supplied by MatTek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mito+dsred2/pmc07676689-95-7-17?v=MatTek
Average 90 stars, based on 1 article reviews
dsred2-mito-7 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


Mitochondria occupy fine astrocyte processes concurrently with astrocyte morphological arborization in vitro . (A) Schematic of astrocyte–neuron co-culture assay. (B) Representative images of rat astrocytes transfected with EGFP (green, top panels) and MitoDsRed (magenta, middle panels) from 4 to 48 h in co-culture with cortical neurons. Inset (bottom panels) of distal astrocyte processes (green) housing mitochondria (magenta) at the leading edge of growing processes (white arrowheads) from 4 to 48 h in culture. Scale bars: 20 μm. (C) Overview of the Seg_Astro image analysis pipeline. (D) Astrocyte secondary, fine/distal, and terminal branch number at 4 vs. 48 h in culture. n = 10–12 cells per time point from one experiment. Unpaired two-tailed t test. (E and F) Total astrocyte mitochondria number and (F) average mitochondrial size in secondary, fine/distal, and terminal astrocyte branches from 4 to 48 h in culture. n = 10–12 cells per time point from one experiment. Data are presented as mean ± SEM. One-way ANOVA with Tukey post hoc test.

Journal: The Journal of Cell Biology

Article Title: Mitochondrial fission controls astrocyte morphogenesis and organization in the cortex

doi: 10.1083/jcb.202410130

Figure Lengend Snippet: Mitochondria occupy fine astrocyte processes concurrently with astrocyte morphological arborization in vitro . (A) Schematic of astrocyte–neuron co-culture assay. (B) Representative images of rat astrocytes transfected with EGFP (green, top panels) and MitoDsRed (magenta, middle panels) from 4 to 48 h in co-culture with cortical neurons. Inset (bottom panels) of distal astrocyte processes (green) housing mitochondria (magenta) at the leading edge of growing processes (white arrowheads) from 4 to 48 h in culture. Scale bars: 20 μm. (C) Overview of the Seg_Astro image analysis pipeline. (D) Astrocyte secondary, fine/distal, and terminal branch number at 4 vs. 48 h in culture. n = 10–12 cells per time point from one experiment. Unpaired two-tailed t test. (E and F) Total astrocyte mitochondria number and (F) average mitochondrial size in secondary, fine/distal, and terminal astrocyte branches from 4 to 48 h in culture. n = 10–12 cells per time point from one experiment. Data are presented as mean ± SEM. One-way ANOVA with Tukey post hoc test.

Article Snippet: MitoDsRed was acquired from Addgene (RRID: Addgene_55838).

Techniques: In Vitro, Co-culture Assay, Transfection, Co-Culture Assay, Two Tailed Test

Drp1-induced mitochondrial fission is required for fine astrocyte process formation and mitochondrial localization in vitro . (A) Schematic of Drp1 in mitochondrial fission. (B) Drp1 domain structure, noting the K38E mutation in the GTPase domain. (C) Representative images of rat astrocytes transfected with shControl or shDrp1 (green) and MitoDsRed (magenta) (top panels) co-cultured on neurons (unlabeled), with a MitoDsRed mask (lower panels), noting hyperfused/elongated mitochondria in shDrp1 astrocytes (red arrowheads). Scale bars: 40 μm. (D) Representative images of rat astrocytes transfected with shRNA targeting Drp1 (shDrp1) or a scrambled control (shControl) (first column) co-cultured on neurons (unlabeled) and their mitochondria (second column) across primary, secondary, fine, and terminal astrocyte process types. Scale bar: 20 μm. (E) Super-plotted quantification of average mitochondrial number in secondary, fine, and terminal branches from shControl vs. shDrp1 astrocytes. (F) Quantification of primary, secondary, fine, and terminal branch numbers from shControl and shDrp1 astrocytes. Data are mean ± SEM n = 4 independent experiments (large circles), 10 cells/condition/experiment (small gray dots). Nested t test. (G) Representative images of rat astrocytes transfected with shControl (green), shDrp1 (green), shDrp1 (green)+ hDrp1-YFP (magenta), or shDrp1 (green)+ hDrp1-K38E-CFP (magenta). Scale bar: 40 μm. (H–K) Quantification of total primary, (I) secondary, (J) fine, and (K) terminal branch number in astrocytes across the 4 conditions from G. Data are mean ± SEM n = 3 independent experiments (large circles), 10 cells/condition/experiment (small gray dots). Nested one-way ANOVA with Tukey post hoc test.

Journal: The Journal of Cell Biology

Article Title: Mitochondrial fission controls astrocyte morphogenesis and organization in the cortex

doi: 10.1083/jcb.202410130

Figure Lengend Snippet: Drp1-induced mitochondrial fission is required for fine astrocyte process formation and mitochondrial localization in vitro . (A) Schematic of Drp1 in mitochondrial fission. (B) Drp1 domain structure, noting the K38E mutation in the GTPase domain. (C) Representative images of rat astrocytes transfected with shControl or shDrp1 (green) and MitoDsRed (magenta) (top panels) co-cultured on neurons (unlabeled), with a MitoDsRed mask (lower panels), noting hyperfused/elongated mitochondria in shDrp1 astrocytes (red arrowheads). Scale bars: 40 μm. (D) Representative images of rat astrocytes transfected with shRNA targeting Drp1 (shDrp1) or a scrambled control (shControl) (first column) co-cultured on neurons (unlabeled) and their mitochondria (second column) across primary, secondary, fine, and terminal astrocyte process types. Scale bar: 20 μm. (E) Super-plotted quantification of average mitochondrial number in secondary, fine, and terminal branches from shControl vs. shDrp1 astrocytes. (F) Quantification of primary, secondary, fine, and terminal branch numbers from shControl and shDrp1 astrocytes. Data are mean ± SEM n = 4 independent experiments (large circles), 10 cells/condition/experiment (small gray dots). Nested t test. (G) Representative images of rat astrocytes transfected with shControl (green), shDrp1 (green), shDrp1 (green)+ hDrp1-YFP (magenta), or shDrp1 (green)+ hDrp1-K38E-CFP (magenta). Scale bar: 40 μm. (H–K) Quantification of total primary, (I) secondary, (J) fine, and (K) terminal branch number in astrocytes across the 4 conditions from G. Data are mean ± SEM n = 3 independent experiments (large circles), 10 cells/condition/experiment (small gray dots). Nested one-way ANOVA with Tukey post hoc test.

Article Snippet: MitoDsRed was acquired from Addgene (RRID: Addgene_55838).

Techniques: In Vitro, Mutagenesis, Transfection, Cell Culture, shRNA, Control