Journal:

Article Title:

doi:

Figure Lengend Snippet: Percentage concentration changes are displayed for the six studied drugs (of abuse) in box-whisker plots; whiskers indicate the 5–95% percentile; within the boxes, the median is displayed; y-axis was cut at 500% for better visibility, but cases above 500% concentration changes were included for the statistics (above 500%: morphine: n = 5; codeine: n = 2; mirtazapine: n = 2; citalopram: n = 2); dotted line indicates 0% change; dashed lines indicate ±20% change region.

Article Snippet: Methanolic solutions of diazepam, nordiazepam, morphine, codeine, mirtazapine and citalopram (1 mg/mL) and the deuterated internal standards (IS) diazepam-d5, nordiazepam-d5, morphine-d3, codeine-d3, mirtazapine-d3, citalopram-d6 and trimipramine-d3 (0.1 mg/mL) were obtained from Cerilliant (delivered by Sigma-Aldrich, Buchs, Switzerland).

Techniques: Concentration Assay, Whisker Assay

Journal:

Article Title:

doi:

Figure Lengend Snippet: Summary data on the parameters for the mixed effect models; RSD refers to the relative standard deviation of lambda upon repeating model creation with a randomly selected training set for 50 times.

Article Snippet: Methanolic solutions of diazepam, nordiazepam, morphine, codeine, mirtazapine and citalopram (1 mg/mL) and the deuterated internal standards (IS) diazepam-d5, nordiazepam-d5, morphine-d3, codeine-d3, mirtazapine-d3, citalopram-d6 and trimipramine-d3 (0.1 mg/mL) were obtained from Cerilliant (delivered by Sigma-Aldrich, Buchs, Switzerland).

Techniques: Standard Deviation

Journal:

Article Title:

doi:

Figure Lengend Snippet: Endogenous compounds/features that were found to have the highest spearman correlation coefficients with the corresponding drugs (of abuse); p -values are corrected for multiple testing using false discovery rate (FDR); it is indicated with which workflow the endogenous compound was found and the identification level according to the chemical analysis working group (CAWG) of the metabolomics standards initiative (MSI) [ 30 ].

Article Snippet: Methanolic solutions of diazepam, nordiazepam, morphine, codeine, mirtazapine and citalopram (1 mg/mL) and the deuterated internal standards (IS) diazepam-d5, nordiazepam-d5, morphine-d3, codeine-d3, mirtazapine-d3, citalopram-d6 and trimipramine-d3 (0.1 mg/mL) were obtained from Cerilliant (delivered by Sigma-Aldrich, Buchs, Switzerland).

Techniques:

Journal:

Article Title:

doi:

Figure Lengend Snippet: Chemical structures of ( a ) morphine, ( b ) codeine, ( c ) mirtazapine, ( d ) citalopram and ( e ) amitriptyline.

Article Snippet: Methanolic solutions of diazepam, nordiazepam, morphine, codeine, mirtazapine and citalopram (1 mg/mL) and the deuterated internal standards (IS) diazepam-d5, nordiazepam-d5, morphine-d3, codeine-d3, mirtazapine-d3, citalopram-d6 and trimipramine-d3 (0.1 mg/mL) were obtained from Cerilliant (delivered by Sigma-Aldrich, Buchs, Switzerland).

Techniques:

Journal: eLife

Article Title: Suppression of transcriptional drift extends C. elegans lifespan by postponing the onset of mortality

doi: 10.7554/eLife.08833

Figure Lengend Snippet: List of small molecules and chemicals used in this study with information DOI: http://dx.doi.org/10.7554/eLife.08833.029

Article Snippet: Mirtazapine , 85650-52-8 , M3368 , LKT Laboratories.

Techniques:

Journal: Nature Communications

Article Title: A degron-mimicking molecular glue drives CRBN homo-dimerization and degradation

doi: 10.1038/s41467-025-65094-3

Figure Lengend Snippet: a Chemical structure of MRT-31619, OUN20985 (CRBN homo-PROTAC) and Lenalidomide. b , c Global quantitative proteomics in Jurkat cells. Volcano plots show compound/DMSO protein abundance upon treatment with 1 μM of MRT-31619 or CRBN homo-PROTAC for 1 h, 3 h and 24 h, and with 10 μM of MRT-31619 or CRBN homo-PROTAC for 24 h. d HEK293T_HiBiT-CRBN cells were treated with MRT-31619 for 1 h. Bortezomib at 2 μM, MLN4924 at 2 μM, and lenalidomide at 20 μM were added 1 h prior to MRT-31619 treatment. Values were normalized to DMSO treatment. Four biological replicates were plotted as mean ± Standard Deviation (SD). e HEK293T_HiBiT-CRBN cells were treated with CRBN homo-PROTAC for 1 h. Bortezomib at 2 μM, MLN4924 at 2 μM, and lenalidomide at 20 μM were added 1 h prior to CRBN homo-PROTAC treatment. Values were normalized to DMSO treatment. Four biological replicates were plotted as mean ± SD. f NanoBRET assay for HaloTag-CRBN and CRBN-NanoLuc constructs upon MRT-31619 or lenalidomide treatment. In the double treatment condition, lenalidomide was added at 20 μM, 1 h prior to MRT-31619 treatment. Values were normalized to DMSO treatment. Three biological replicates were plotted as mean ± Standard Error of Measurement (SEM). g NanoBRET assay for HaloTag-CRBN and CRBN-NanoLuc (CRBN-Nluc) wild-type (WT) and W386A constructs upon MRT-31619 treatment. Values were normalized to DMSO treatment. Three biological replicates were plotted as mean ± SEM. Source data are provided as a Source Data file.

Article Snippet: Cells were treated for 3 h with DMSO (Sigma Cat#41639) at 0.1% final concentration, MRT compounds or lenalidomide (Selleckchem Cat#S1029) at serial dilution using a Tecan D300e Digital Dispenser.

Techniques: Quantitative Proteomics, Standard Deviation, Construct

Journal: Nature Communications

Article Title: A degron-mimicking molecular glue drives CRBN homo-dimerization and degradation

doi: 10.1038/s41467-025-65094-3

Figure Lengend Snippet: a The cis CRBN (CRBN 1 ) binds one MRT-31619 molecule (MGD 1 ) at the tri-tryptophan pocket and interacts with the trans MRT-31619 molecule (MGD 2 ) via a hydrogen bond to W400. b CRBN W400 interacts with the CK1α G-loop via a hydrogen bond in the presence of lenalidomide. c Superposition of panels a and b indicates that MGD 2 is a structural mimic of the CK1α G-loop and employs the same hydrogen bond. d – f Chemical structures of MRT-31619, MRT-31015, or MRT-30568 and their corresponding global quantitative proteomics data in Jurkat cells. Volcano plots show compound/DMSO protein abundance upon treatment with 10 μM of MRT-31619, MRT-31015, or MRT-30568 for 24 h. g HEK293T_HiBiT-CRBN degradation upon MRT-31619, MRT-31015, or MRT-30568 treatment for 1 h. Four biological replicates were plotted as mean ± SD. h NanoBRET assay for HaloTag-CRBN and CRBN-NanoLuc constructs upon MRT-31619, MRT-31015, or MRT-30568 treatment. Three biological replicates were plotted as mean ± SEM. i Flow-induced dispersion analysis (FIDA) for MRT-31619, MRT-31015, or MRT-30568 and the corresponding EC 50 and Max R h values. The predicted value of R h calculated from the cryo-EM structure of the ternary complex is 7.47 nm. Three biological replicates were plotted as mean ± SD. Source data are provided as a Source Data file.

Article Snippet: Cells were treated for 3 h with DMSO (Sigma Cat#41639) at 0.1% final concentration, MRT compounds or lenalidomide (Selleckchem Cat#S1029) at serial dilution using a Tecan D300e Digital Dispenser.

Techniques: Quantitative Proteomics, Construct, Dispersion, Cryo-EM Sample Prep

Journal: Journal of Analytical Toxicology

Article Title: Determination of Seven Antidepressants in Pericardial Fluid by Means of Dispersive Liquid–Liquid Microextraction and Gas Chromatography–Mass Spectrometry

doi: 10.1093/jat/bkab003

Figure Lengend Snippet: Retention Times and Ions Selected for Monitorization

Article Snippet: Fluoxetine, venlafaxine, mirtazapine, sertraline, citalopram, olanzapine, paroxetine and proadifen (SKF), used as internal standard, were obtained from Cerilliant ® .

Techniques:

Journal: Journal of Analytical Toxicology

Article Title: Determination of Seven Antidepressants in Pericardial Fluid by Means of Dispersive Liquid–Liquid Microextraction and Gas Chromatography–Mass Spectrometry

doi: 10.1093/jat/bkab003

Figure Lengend Snippet: LOD, Limit of Quantification and Calibration Results for the Antidepressants Studied

Article Snippet: Fluoxetine, venlafaxine, mirtazapine, sertraline, citalopram, olanzapine, paroxetine and proadifen (SKF), used as internal standard, were obtained from Cerilliant ® .

Techniques:

Journal: Journal of Analytical Toxicology

Article Title: Determination of Seven Antidepressants in Pericardial Fluid by Means of Dispersive Liquid–Liquid Microextraction and Gas Chromatography–Mass Spectrometry

doi: 10.1093/jat/bkab003

Figure Lengend Snippet: Intra- and Inter-Day Precision and Accuracy (ME) of the Method

Article Snippet: Fluoxetine, venlafaxine, mirtazapine, sertraline, citalopram, olanzapine, paroxetine and proadifen (SKF), used as internal standard, were obtained from Cerilliant ® .

Techniques:

Journal: Journal of Analytical Toxicology

Article Title: Determination of Seven Antidepressants in Pericardial Fluid by Means of Dispersive Liquid–Liquid Microextraction and Gas Chromatography–Mass Spectrometry

doi: 10.1093/jat/bkab003

Figure Lengend Snippet: Intra and Inter-Day Accuracy (Analytical Recovery) of the Method

Article Snippet: Fluoxetine, venlafaxine, mirtazapine, sertraline, citalopram, olanzapine, paroxetine and proadifen (SKF), used as internal standard, were obtained from Cerilliant ® .

Techniques:

Journal: Journal of Analytical Toxicology

Article Title: Determination of Seven Antidepressants in Pericardial Fluid by Means of Dispersive Liquid–Liquid Microextraction and Gas Chromatography–Mass Spectrometry

doi: 10.1093/jat/bkab003

Figure Lengend Snippet: Real Cases

Article Snippet: Fluoxetine, venlafaxine, mirtazapine, sertraline, citalopram, olanzapine, paroxetine and proadifen (SKF), used as internal standard, were obtained from Cerilliant ® .

Techniques:

Journal: International Journal of Methods in Psychiatric Research

Article Title: Measuring psychotropic drug exposures in register‐based studies – validity of a dosage assumption of one unit per day in older Finns

doi: 10.1002/mpr.1384

Figure Lengend Snippet: The categories and subcategories of psychotropic drugs

Article Snippet: For instance, the lower validity of the dosage assumption among other antidepressants prescribed for insomnia may result from the fact that guidelines recommend a dose of 3.75 to 7.5 mg of mirtazapine for insomnia (Finnish Medical Society Duodecim, 2008 ), even though the lowest strength available is 15 mg.

Techniques:

Journal: International Journal of Methods in Psychiatric Research

Article Title: Measuring psychotropic drug exposures in register‐based studies – validity of a dosage assumption of one unit per day in older Finns

doi: 10.1002/mpr.1384

Figure Lengend Snippet: The percentages of prescriptions with a prescribed dose below or above one unit per day and defined daily dose (DDD) per day

Article Snippet: For instance, the lower validity of the dosage assumption among other antidepressants prescribed for insomnia may result from the fact that guidelines recommend a dose of 3.75 to 7.5 mg of mirtazapine for insomnia (Finnish Medical Society Duodecim, 2008 ), even though the lowest strength available is 15 mg.

Techniques: