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min09i 33114 c control ipsc  (WiCell Research Institute Inc)
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WiCell Research Institute Inc min09i 33114 c control ipsc
Summary of selected neural organoid protocols with specific patterning manipulations to LGE/Striatal and MGE subpallial subregions. Cell type identification used the reported gene expressions of each protocol to broadly classify the types of cells that might be found within each organoid.
Min09i 33114 C Control Ipsc, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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min09i-33114.c.b  (WiCell Research Institute Inc)
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WiCell Research Institute Inc min09i-33114.c.b
Summary of selected neural organoid protocols with specific patterning manipulations to LGE/Striatal and MGE subpallial subregions. Cell type identification used the reported gene expressions of each protocol to broadly classify the types of cells that might be found within each organoid.
Min09i 33114.C.B, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 89 stars, based on 1 article reviews
min09i-33114.c.b - by Bioz Stars, 2026-02
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Summary of selected neural organoid protocols with specific patterning manipulations to LGE/Striatal and MGE subpallial subregions. Cell type identification used the reported gene expressions of each protocol to broadly classify the types of cells that might be found within each organoid.

Journal: Frontiers in Genetics

Article Title: Cell type specification and diversity in subpallial organoids

doi: 10.3389/fgene.2024.1440583

Figure Lengend Snippet: Summary of selected neural organoid protocols with specific patterning manipulations to LGE/Striatal and MGE subpallial subregions. Cell type identification used the reported gene expressions of each protocol to broadly classify the types of cells that might be found within each organoid.

Article Snippet: , IHC, RT-qPCR, Western blot, MEA, scRNA-seq , Four patient derived iPSCs, four healthy donor iPSCs, MIN09i-33114.C control iPSC (WiCell) , D6-30: Pur (0.65 µM) + 100 ng DKK-1 , Early markers: Early LGE (MEIS2), MGE (NKX2.1), CGE (NR2F1, NR2F2, SP8), Neural progenitors (SOX2, Ki67), Forebrain (FOXG1) Late markers: Late LGE (FOXP2, POU3F1), Developing MSN (CTIP2), Developing MSN2 (DARPP32, CALB1), Pre D1-MSN (TAC1, EBF1), Pre D2-MSN (SIX3, GRIK3), D1-MSN (IKZF1, PDYN, DRD1), D2-MSN (EGR3, PENK, ADORA2A, DRD2), Striatal INs (EPHB1, NKX2.1, EPHB3, CR), Cortical INs (NRP1, RELN, LHX6, HTR3A), Neocortex (EOMES, TBR1, SATB2), Astrocyte (GFAP, S100b) , Mature MSNs and INs were rarely detected in this protocol suggesting immaturity in ventral forebrain organoids. INs expressed marker genes for striosome and matrix. No unique cell types generated between D70 and D150. However, an upregulation of synaptic genes was observed in at D150. Additionally, increased synchronous activity and firing rate observed at D150 through MEA..

Techniques: Transplantation Assay, Optogenetics, Imaging, Patch Clamp, Control, Derivative Assay, Activity Assay, Western Blot, RNA Sequencing Assay, In Vitro, Marker, Generated, Migration, In Vivo