mimic negative control Search Results


mir 1183 inhibitor  (MedChemExpress)
95
MedChemExpress mir 1183 inhibitor
DDX11-AS1 competitively <t>binds</t> <t>miR-1183</t> through competing endogenous RNA (ceRNA) mechanism. ( A ) In silico analysis of the binding site of miR-1183 within DDX11-AS1 sequence. ( B ) Correlation between DDX11-AS1 and miR-1183 expression in clinical glioma samples ( n = 32). ( C , D ) The expression level of miR-1183 in glioma cells with DDX11-AS1 ( C ) knockdown or ( D ) overexpression ( n = 6). ( E ) The relative luciferase activity was examined in U87 glioma cells co-transfected with DDX11-AS1 wild-type (WT) or DDX11-AS1 mutant (MUT) and miR-1183 mimics or the miR-NC. ( F , G ) RIP assay in ( F ) U87 and ( G ) U251 glioma cells. ( H ) Binding of miR-1183 and miR-NC to DDX11-AS1 in the RNA pull-down assay (GAPDH as control). ( I ) RNA FISH for DDX11-AS1 and miR-1183 in human glioma sample. Data are expressed as the mean ± SEM, with statistical significance (* p < 0.05) determined by Student’s t test
Mir 1183 Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/MicroRNA+Mimic+Negative+Control/pmc12494103-52-33-37
Average 95 stars, based on 1 article reviews
mir 1183 inhibitor - by Bioz Stars, 2026-10
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mimics, inhibitor negative controls hsa-mir-1306-3p  (Ribobio co)
90
Ribobio co mimics, inhibitor negative controls hsa-mir-1306-3p
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Mimics, Inhibitor Negative Controls Hsa Mir 1306 3p, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/mimics++inhibitor+negative+controls+hsa+mir+1306+3p/pmc06704702-112-7-11
Average 90 stars, based on 1 article reviews
mimics, inhibitor negative controls hsa-mir-1306-3p - by Bioz Stars, 2026-10
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negative control mimic  (Ribobio co)
90
Ribobio co negative control mimic
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Negative Control Mimic, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/mimic+negative+control/pm30365049-48-15-31
Average 90 stars, based on 1 article reviews
negative control mimic - by Bioz Stars, 2026-10
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negative control (nc) mimics  (Shanghai GenePharma)
90
Shanghai GenePharma negative control (nc) mimics
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Negative Control (Nc) Mimics, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/mimic+negative+control/pmc08661150-87-7-17
Average 90 stars, based on 1 article reviews
negative control (nc) mimics - by Bioz Stars, 2026-10
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mimics negative control precursor (nc)  (Shanghai GenePharma)
90
Shanghai GenePharma mimics negative control precursor (nc)
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Mimics Negative Control Precursor (Nc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/mimics+negative+control+precursor++nc+/pm27878247-49-4-20
Average 90 stars, based on 1 article reviews
mimics negative control precursor (nc) - by Bioz Stars, 2026-10
90/100 stars
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50 nmol/l mimic-negative control (mimic-nc)  (Ribobio co)
90
Ribobio co 50 nmol/l mimic-negative control (mimic-nc)
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
50 Nmol/L Mimic Negative Control (Mimic Nc), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/50+nmol+l+mimic+negative+control++mimic+nc+/pmc06961604-83-19-5
Average 90 stars, based on 1 article reviews
50 nmol/l mimic-negative control (mimic-nc) - by Bioz Stars, 2026-10
90/100 stars
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mirna negative control mir01102-1-1  (Ribobio co)
90
Ribobio co mirna negative control mir01102-1-1
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Mirna Negative Control Mir01102 1 1, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/negative+control+of+mimic++mc++mir01102+1+1+/pmc10767360-112-17-21
Average 90 stars, based on 1 article reviews
mirna negative control mir01102-1-1 - by Bioz Stars, 2026-10
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negative control mimics or inhibitor  (Ribobio co)
90
Ribobio co negative control mimics or inhibitor
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Negative Control Mimics Or Inhibitor, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/negative+control+mimics+or+inhibitor/pm24446471-89-4-5
Average 90 stars, based on 1 article reviews
negative control mimics or inhibitor - by Bioz Stars, 2026-10
90/100 stars
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single-strand mimics or negative control of mimics (mimics-nc)  (Ribobio co)
90
Ribobio co single-strand mimics or negative control of mimics (mimics-nc)
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Single Strand Mimics Or Negative Control Of Mimics (Mimics Nc), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/single+strand+mimics+or+negative+control+of+mimics++mimics+nc+/10__2147_slash_cmar__s363629-60-12-14
Average 90 stars, based on 1 article reviews
single-strand mimics or negative control of mimics (mimics-nc) - by Bioz Stars, 2026-10
90/100 stars
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mimic negative control (nc)  (Promega)
90
Promega mimic negative control (nc)
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Mimic Negative Control (Nc), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/mimic+negative+control++nc+/10__2147_slash_ijgm__s272996-98-17-6
Average 90 stars, based on 1 article reviews
mimic negative control (nc) - by Bioz Stars, 2026-10
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negative control mirna mimics pmir-control  (GenScript corporation)
90
GenScript corporation negative control mirna mimics pmir-control
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Negative Control Mirna Mimics Pmir Control, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/negative+control+mirna+mimics+pmir+control/pm31059098-29-37-42
Average 90 stars, based on 1 article reviews
negative control mirna mimics pmir-control - by Bioz Stars, 2026-10
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mimics negative control microntm mimics negative control#22  (Phalanx Biotech)
90
Phalanx Biotech mimics negative control microntm mimics negative control#22
CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of <t>miR-1306-3p.</t> a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01
Mimics Negative Control Microntm Mimics Negative Control#22, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mimic+negative+control/mimics+negative+control+microntm+mimics+negative+control+22/pmc03420912-160-46-52
Average 90 stars, based on 1 article reviews
mimics negative control microntm mimics negative control#22 - by Bioz Stars, 2026-10
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Image Search Results


DDX11-AS1 competitively binds miR-1183 through competing endogenous RNA (ceRNA) mechanism. ( A ) In silico analysis of the binding site of miR-1183 within DDX11-AS1 sequence. ( B ) Correlation between DDX11-AS1 and miR-1183 expression in clinical glioma samples ( n = 32). ( C , D ) The expression level of miR-1183 in glioma cells with DDX11-AS1 ( C ) knockdown or ( D ) overexpression ( n = 6). ( E ) The relative luciferase activity was examined in U87 glioma cells co-transfected with DDX11-AS1 wild-type (WT) or DDX11-AS1 mutant (MUT) and miR-1183 mimics or the miR-NC. ( F , G ) RIP assay in ( F ) U87 and ( G ) U251 glioma cells. ( H ) Binding of miR-1183 and miR-NC to DDX11-AS1 in the RNA pull-down assay (GAPDH as control). ( I ) RNA FISH for DDX11-AS1 and miR-1183 in human glioma sample. Data are expressed as the mean ± SEM, with statistical significance (* p < 0.05) determined by Student’s t test

Journal: Oncology Research

Article Title: Competitive Sequestration of miR-1183 by lncRNA DDX11-AS1 Drives Gliomagenesis through E2F7 Activation

doi: 10.32604/or.2025.065380

Figure Lengend Snippet: DDX11-AS1 competitively binds miR-1183 through competing endogenous RNA (ceRNA) mechanism. ( A ) In silico analysis of the binding site of miR-1183 within DDX11-AS1 sequence. ( B ) Correlation between DDX11-AS1 and miR-1183 expression in clinical glioma samples ( n = 32). ( C , D ) The expression level of miR-1183 in glioma cells with DDX11-AS1 ( C ) knockdown or ( D ) overexpression ( n = 6). ( E ) The relative luciferase activity was examined in U87 glioma cells co-transfected with DDX11-AS1 wild-type (WT) or DDX11-AS1 mutant (MUT) and miR-1183 mimics or the miR-NC. ( F , G ) RIP assay in ( F ) U87 and ( G ) U251 glioma cells. ( H ) Binding of miR-1183 and miR-NC to DDX11-AS1 in the RNA pull-down assay (GAPDH as control). ( I ) RNA FISH for DDX11-AS1 and miR-1183 in human glioma sample. Data are expressed as the mean ± SEM, with statistical significance (* p < 0.05) determined by Student’s t test

Article Snippet: All cell cultures underwent validation via short tandem repeat profiling and confirmed absence of mycoplasma contamination. miR-1183 mimics (catalog #HY-R00073; MCE, Monmouth Junction, NJ, USA), miRNA negative controls (miR-NC; catalog #HY-R04602; MCE) and miR-1183 inhibitor (catalog #HY-RI00073; MCE) were acquired from MCE.

Techniques: In Silico, Binding Assay, Sequencing, Expressing, Knockdown, Over Expression, Luciferase, Activity Assay, Transfection, Mutagenesis, Pull Down Assay, Control

DDX11-AS1 promotes glioma cell proliferation and migration by targeting miR-1183. Glioma cells transfected with sh-NC, sh-DDX11-AS1 or sh-DDX11-AS1 followed by miR-1183 inhibitor treatment were used. ( A , B ) CCK-8 assays of ( A ) U87 and ( B ) U251 glioma cells ( n = 6). ( C , D ) Colony formation assays of ( C ) U87 and ( D ) U251 glioma cells ( n = 6). ( E ) Scratch wound healing assay of U87 and U251 glioma cells. ( F ) Transwell assay of U87 and U251 glioma cells. The right panel is the quantified data depicting migrated cell number ( n = 6). All data are represented as the mean ± SEM, * p < 0.05, sh-DDX11-AS1 vs. sh-NC; # p < 0.05, sh-DDX11-AS1 vs. sh-DDX11-AS1 + miR-1183 inhibitor (One-way ANOVA)

Journal: Oncology Research

Article Title: Competitive Sequestration of miR-1183 by lncRNA DDX11-AS1 Drives Gliomagenesis through E2F7 Activation

doi: 10.32604/or.2025.065380

Figure Lengend Snippet: DDX11-AS1 promotes glioma cell proliferation and migration by targeting miR-1183. Glioma cells transfected with sh-NC, sh-DDX11-AS1 or sh-DDX11-AS1 followed by miR-1183 inhibitor treatment were used. ( A , B ) CCK-8 assays of ( A ) U87 and ( B ) U251 glioma cells ( n = 6). ( C , D ) Colony formation assays of ( C ) U87 and ( D ) U251 glioma cells ( n = 6). ( E ) Scratch wound healing assay of U87 and U251 glioma cells. ( F ) Transwell assay of U87 and U251 glioma cells. The right panel is the quantified data depicting migrated cell number ( n = 6). All data are represented as the mean ± SEM, * p < 0.05, sh-DDX11-AS1 vs. sh-NC; # p < 0.05, sh-DDX11-AS1 vs. sh-DDX11-AS1 + miR-1183 inhibitor (One-way ANOVA)

Article Snippet: All cell cultures underwent validation via short tandem repeat profiling and confirmed absence of mycoplasma contamination. miR-1183 mimics (catalog #HY-R00073; MCE, Monmouth Junction, NJ, USA), miRNA negative controls (miR-NC; catalog #HY-R04602; MCE) and miR-1183 inhibitor (catalog #HY-RI00073; MCE) were acquired from MCE.

Techniques: Migration, Transfection, CCK-8 Assay, Wound Healing Assay, Transwell Assay

E2F7 is a target of miR-1183. ( A ) The bioinformatics analysis tools were used to predict the binding site of miR-1183 in E2F7. ( B ) Correlation between miR-1183 and E2F7 expression. ( C ) Luciferase activity in U87 cells co-transfected with miR-1183 and WT/MUT E2F7 reporter constructs ( n = 6). ( D – G ) U87 and U251 cells transfected with miR-1183, miR-NC or E2F7-overexpression vectors were used; ( D , E ) Relative expression of E2F7 was determined by RT-qPCR ( n = 6); ( F , G ) CCK-8 assay of ( F ) U87 and ( G ) U251 cells ( n = 6); ( H , I ) U87 and U251 cells transfected with sh-NC, sh-DDX11-AS1 or sh-DDX11-AS1 followed with miR-1183 inhibitor treatment were used. ( H ) Relative expression of E2F7 was determined by RT-qPCR ( n = 6). ( I ) Immunoblotting assays detected E2F7 and GAPDH in U87 and U251 cells. The bar chart quantifies relative E2F7 protein abundance normalized to GAPDH ( n = 6). All data are represented as the mean ± SEM, * p < 0.05, sh-DDX11-AS1 vs. sh-NC; # p < 0.05, sh-DDX11-AS1 vs. sh-DDX11-AS1 + miR-1183 inhibitor (One-way ANOVA)

Journal: Oncology Research

Article Title: Competitive Sequestration of miR-1183 by lncRNA DDX11-AS1 Drives Gliomagenesis through E2F7 Activation

doi: 10.32604/or.2025.065380

Figure Lengend Snippet: E2F7 is a target of miR-1183. ( A ) The bioinformatics analysis tools were used to predict the binding site of miR-1183 in E2F7. ( B ) Correlation between miR-1183 and E2F7 expression. ( C ) Luciferase activity in U87 cells co-transfected with miR-1183 and WT/MUT E2F7 reporter constructs ( n = 6). ( D – G ) U87 and U251 cells transfected with miR-1183, miR-NC or E2F7-overexpression vectors were used; ( D , E ) Relative expression of E2F7 was determined by RT-qPCR ( n = 6); ( F , G ) CCK-8 assay of ( F ) U87 and ( G ) U251 cells ( n = 6); ( H , I ) U87 and U251 cells transfected with sh-NC, sh-DDX11-AS1 or sh-DDX11-AS1 followed with miR-1183 inhibitor treatment were used. ( H ) Relative expression of E2F7 was determined by RT-qPCR ( n = 6). ( I ) Immunoblotting assays detected E2F7 and GAPDH in U87 and U251 cells. The bar chart quantifies relative E2F7 protein abundance normalized to GAPDH ( n = 6). All data are represented as the mean ± SEM, * p < 0.05, sh-DDX11-AS1 vs. sh-NC; # p < 0.05, sh-DDX11-AS1 vs. sh-DDX11-AS1 + miR-1183 inhibitor (One-way ANOVA)

Article Snippet: All cell cultures underwent validation via short tandem repeat profiling and confirmed absence of mycoplasma contamination. miR-1183 mimics (catalog #HY-R00073; MCE, Monmouth Junction, NJ, USA), miRNA negative controls (miR-NC; catalog #HY-R04602; MCE) and miR-1183 inhibitor (catalog #HY-RI00073; MCE) were acquired from MCE.

Techniques: Binding Assay, Expressing, Luciferase, Activity Assay, Transfection, Construct, Over Expression, Quantitative RT-PCR, CCK-8 Assay, Western Blot, Quantitative Proteomics

CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of miR-1306-3p. a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01

Journal: Molecular Cancer

Article Title: Circular RNA circNHSL1 promotes gastric cancer progression through the miR-1306-3p/SIX1/vimentin axis

doi: 10.1186/s12943-019-1054-7

Figure Lengend Snippet: CircNHSL1 promotes gastric cancer progression by serving as a miRNA sponge of miR-1306-3p. a Pearson correlation analysis determined the significantly negative correlation between the levels of circNHSL1 and miR-1306-3p in 61 paired gastric cancer tissues (p < 0.01). b The effects of circNHSL1 on the expression of SIX1 was detected by qRT-PCR. c The effects of mimics, inhibitor and negative control on the luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of WT, Mutant or negative control. d Schematic illustration of the sequence of wild type of SIX1 3′-UTR (WT) and mutant sequences on the complementary sites of SIX1 3′-UTR with miR-1306-3p (Mutant). e and f Anti-AGO2 RIP assay was performed in MKN-28 cells after transfection with mimics or negative control, followed by agarose gel electrophoresis ( e ) and qRT-PCR ( f ) to detect the expression of circNHSL1 and miR-1306-3p. g The effects of circNHSL1 and miR-1306-3p on the luciferase activities of wild type of SIX1 mRNA 3′-UTR in gastric cancer cells were detected. h and i The effects of circNHSL1 and miR-1306-3p on the mRNA and protein expressions of SIX1 and Vimentin were detected by qRT-PCR and western blotting. j and k The effects of circNHSL1 and miR-1306-3p on the mobility, migration and invasion were confirmed by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. ** p < 0.01

Article Snippet: The mimics, inhibitor and negative controls for hsa-miR-1306-3p were purchased from RiBoBio (Guangzhou, China).

Techniques: Expressing, Quantitative RT-PCR, Negative Control, Luciferase, Mutagenesis, Sequencing, Transfection, Agarose Gel Electrophoresis, Western Blot, Migration

MiR-1306-3p is down-regulated in gastric cancer tissues and correlated with the progression and poor prognosis. The expression of miR-1306-3p was detected by ISH with TMA including 54 paired gastric cancer tissues and paired normal tissues. a Represent images of miR-1306-3p expression in normal gastric mucosa and gastric cancer tissues with Stage II, Stage III, M0, M1, G1, G2, G3, pT2, pT3 and pT4. b The level of miR-1306-3p in gastric cancer tissues was significantly lower than in normal gastric tissues. c-f The level of miR-1306-3p in gastric cancer tissues with Stage II ( c ), M0 ( d ), well differentiation ( e ) and pT2 and pT3 ( f ) was significantly higher than these with Stage III, M1, moderate and poor differentiation and pT4. g - h Kaplan-Meier survival analysis (log-rank test) showed that gastric cancer patients with low miR-1306-3p expression have a lower OS and DFS than these with high miR-1306-3p expression ( p < 0.01). All data are presented as the mean ± SEM. ** p < 0.01

Journal: Molecular Cancer

Article Title: Circular RNA circNHSL1 promotes gastric cancer progression through the miR-1306-3p/SIX1/vimentin axis

doi: 10.1186/s12943-019-1054-7

Figure Lengend Snippet: MiR-1306-3p is down-regulated in gastric cancer tissues and correlated with the progression and poor prognosis. The expression of miR-1306-3p was detected by ISH with TMA including 54 paired gastric cancer tissues and paired normal tissues. a Represent images of miR-1306-3p expression in normal gastric mucosa and gastric cancer tissues with Stage II, Stage III, M0, M1, G1, G2, G3, pT2, pT3 and pT4. b The level of miR-1306-3p in gastric cancer tissues was significantly lower than in normal gastric tissues. c-f The level of miR-1306-3p in gastric cancer tissues with Stage II ( c ), M0 ( d ), well differentiation ( e ) and pT2 and pT3 ( f ) was significantly higher than these with Stage III, M1, moderate and poor differentiation and pT4. g - h Kaplan-Meier survival analysis (log-rank test) showed that gastric cancer patients with low miR-1306-3p expression have a lower OS and DFS than these with high miR-1306-3p expression ( p < 0.01). All data are presented as the mean ± SEM. ** p < 0.01

Article Snippet: The mimics, inhibitor and negative controls for hsa-miR-1306-3p were purchased from RiBoBio (Guangzhou, China).

Techniques: Expressing