middlebrook oadc enrichment Search Results


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  • 99
    Millipore oadc enrichment
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    MiddleBrook Pharmaceuticals oadc enriched middlebrook 7h9 broth
    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    MiddleBrook Pharmaceuticals oadc enriched 7h10 middlebrook agar plates
    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Image Search Results


    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 Middlebrook with 10% of OADC, with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).

    Journal: PLoS ONE

    Article Title: Either Non-Homologous Ends Joining or Homologous Recombination Is Required to Repair Double-Strand Breaks in the Genome of Macrophage-Internalized Mycobacterium tuberculosis

    doi: 10.1371/journal.pone.0092799

    Figure Lengend Snippet: Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 Middlebrook with 10% of OADC, with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).

    Article Snippet: Middlebrook 7H10 agar, Middlebrook 7H9 broth, and Middlebrook OADC enrichment were acquired from Becton Dickinson (Franklin Lakes, NJ, USA).

    Techniques: Cell Culture, Inhibition

    Effect of inhibition of ROS and NO production on the survival of Mtb strains inside MØs. MØs were incubated with apocynin or L-NIL for 30 minutes, then infected with Mtb wild-type or Δ( ku,ligD,recA ) for 2 hours (MOI = 10) and washed with HBSS. On the day of infection and after 6 days of culture, MØs were lysed with Triton X-100 and cell lysates were plated onto Middlebrook 7H10 agar supplemented with 10% OADC. After 21 days of culture, CFUs were counted. Data are presented as fold increase in CFUs, expressed as means ± SEM (n = 5; #p≤0.04, Δ( ku,ligD,recA ) vs. Δ( ku,ligD,recA ) + apocynin or L-NIL; Mann-Whitney U test).

    Journal: PLoS ONE

    Article Title: Either Non-Homologous Ends Joining or Homologous Recombination Is Required to Repair Double-Strand Breaks in the Genome of Macrophage-Internalized Mycobacterium tuberculosis

    doi: 10.1371/journal.pone.0092799

    Figure Lengend Snippet: Effect of inhibition of ROS and NO production on the survival of Mtb strains inside MØs. MØs were incubated with apocynin or L-NIL for 30 minutes, then infected with Mtb wild-type or Δ( ku,ligD,recA ) for 2 hours (MOI = 10) and washed with HBSS. On the day of infection and after 6 days of culture, MØs were lysed with Triton X-100 and cell lysates were plated onto Middlebrook 7H10 agar supplemented with 10% OADC. After 21 days of culture, CFUs were counted. Data are presented as fold increase in CFUs, expressed as means ± SEM (n = 5; #p≤0.04, Δ( ku,ligD,recA ) vs. Δ( ku,ligD,recA ) + apocynin or L-NIL; Mann-Whitney U test).

    Article Snippet: Middlebrook 7H10 agar, Middlebrook 7H9 broth, and Middlebrook OADC enrichment were acquired from Becton Dickinson (Franklin Lakes, NJ, USA).

    Techniques: Inhibition, Incubation, Infection, MANN-WHITNEY

    Survival of Mtb strains inside MØs. (A) MØs were infected with Mtb wild-type or its mutants, Δ( ku,ligD,recA ), Δ( ku,ligD ) or Δ recA , for 2 hours (MOI = 10), washed with HBSS, and cultured for 2, 4, and 6 days. (B and C) MØs were infected with Mtb wild-type or its mutants (B) or complemented strains Δ( ku,ligD,recA )- P recA recA – complemented strain I or Δ( ku,ligD,recA )- P kuligD ku-ligD – complemented strain II (C) for 6 days. On the day of infection and after 6 days of culture, MØs were lysed with Triton X-100 and cell lysates were plated onto Middlebrook 7H10 agar supplemented with 10% OADC. After 21 days of culture, CFUs were counted. Data are presented as fold increase in CFUs, expressed as means ± SEM (n = 5; *p≤0.05, Δ( ku,ligD,recA ) vs. wild-type; #p≤0.05, complemented strain I and II vs. Δ( ku,ligD,recA ) Mann-Whitney U test).

    Journal: PLoS ONE

    Article Title: Either Non-Homologous Ends Joining or Homologous Recombination Is Required to Repair Double-Strand Breaks in the Genome of Macrophage-Internalized Mycobacterium tuberculosis

    doi: 10.1371/journal.pone.0092799

    Figure Lengend Snippet: Survival of Mtb strains inside MØs. (A) MØs were infected with Mtb wild-type or its mutants, Δ( ku,ligD,recA ), Δ( ku,ligD ) or Δ recA , for 2 hours (MOI = 10), washed with HBSS, and cultured for 2, 4, and 6 days. (B and C) MØs were infected with Mtb wild-type or its mutants (B) or complemented strains Δ( ku,ligD,recA )- P recA recA – complemented strain I or Δ( ku,ligD,recA )- P kuligD ku-ligD – complemented strain II (C) for 6 days. On the day of infection and after 6 days of culture, MØs were lysed with Triton X-100 and cell lysates were plated onto Middlebrook 7H10 agar supplemented with 10% OADC. After 21 days of culture, CFUs were counted. Data are presented as fold increase in CFUs, expressed as means ± SEM (n = 5; *p≤0.05, Δ( ku,ligD,recA ) vs. wild-type; #p≤0.05, complemented strain I and II vs. Δ( ku,ligD,recA ) Mann-Whitney U test).

    Article Snippet: Middlebrook 7H10 agar, Middlebrook 7H9 broth, and Middlebrook OADC enrichment were acquired from Becton Dickinson (Franklin Lakes, NJ, USA).

    Techniques: Infection, Cell Culture, MANN-WHITNEY