microscope software zen2 Search Results


algorithms zen2 software carl zeiss n a odyssey clx imaging system li cor biosciences n a image j nih  (LI-COR)
99
LI-COR algorithms zen2 software carl zeiss n a odyssey clx imaging system li cor biosciences n a image j nih
Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss <t>Zen2</t> software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The
Algorithms Zen2 Software Carl Zeiss N A Odyssey Clx Imaging System Li Cor Biosciences N A Image J Nih, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+software+zen2/pm37378334-140-58-68?v=LI-COR
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algorithms zen2 software carl zeiss n a odyssey clx imaging system li cor biosciences n a image j nih - by Bioz Stars, 2026-08
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csu xa1 spinning disk confocal head  (Yokogawa Electric)
99
Yokogawa Electric csu xa1 spinning disk confocal head
Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss <t>Zen2</t> software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The
Csu Xa1 Spinning Disk Confocal Head, supplied by Yokogawa Electric, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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csu xa1 spinning disk confocal head - by Bioz Stars, 2026-08
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zeiss zen 2 3 blue  (Oxford Instruments)
99
Oxford Instruments zeiss zen 2 3 blue
Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss <t>Zen2</t> software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The
Zeiss Zen 2 3 Blue, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+software+zen2/pm36297275-117-6-15?v=Oxford+Instruments
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zeiss zen 2 3 blue - by Bioz Stars, 2026-08
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zen 2 5 software image analysis module  (Carl Zeiss)
95
Carl Zeiss zen 2 5 software image analysis module
Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss <t>Zen2</t> software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The
Zen 2 5 Software Image Analysis Module, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+software+zen2/pm39915484-322-2-1?v=Carl+Zeiss
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zen 2 5 software image analysis module - by Bioz Stars, 2026-08
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origin 9.1  (OriginLab corp)
90
OriginLab corp origin 9.1
Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss <t>Zen2</t> software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The
Origin 9.1, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+software+zen2/pm29805038-470-103-117?v=OriginLab+corp
Average 90 stars, based on 1 article reviews
origin 9.1 - by Bioz Stars, 2026-08
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zen lite software  (Carl Zeiss)
96
Carl Zeiss zen lite software
Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss <t>Zen2</t> software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The
Zen Lite Software, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+software+zen2/pmc08004631-69-30-35?v=Carl+Zeiss
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axio imager d2 fluorescence microscope  (Carl Zeiss)
96
Carl Zeiss axio imager d2 fluorescence microscope
Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss <t>Zen2</t> software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The
Axio Imager D2 Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+software+zen2/pmc11434852-174-47-55?v=Carl+Zeiss
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axio observer microscope  (Carl Zeiss)
99
Carl Zeiss axio observer microscope
Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss <t>Zen2</t> software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The
Axio Observer Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+software+zen2/pmc07650833-200-2-1?v=Carl+Zeiss
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171stnd01 zen 2 software zeiss  (Bio-Rad)
95
Bio-Rad 171stnd01 zen 2 software zeiss
Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss <t>Zen2</t> software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The
171stnd01 Zen 2 Software Zeiss, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ca rrid scr 002798  (Inscopix Inc)
86
Inscopix Inc ca rrid scr 002798
Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss <t>Zen2</t> software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The
Ca Rrid Scr 002798, supplied by Inscopix Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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en uk product image labsoftware hokawo 2 5 hamamatsu carl zeiss zen 2 1  (Bio-Rad)
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Bio-Rad en uk product image labsoftware hokawo 2 5 hamamatsu carl zeiss zen 2 1
Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss <t>Zen2</t> software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The
En Uk Product Image Labsoftware Hokawo 2 5 Hamamatsu Carl Zeiss Zen 2 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zenpro software  (Carl Zeiss)
92
Carl Zeiss zenpro software

Zenpro Software, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss Zen2 software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The

Journal: iScience

Article Title: AKT2-mediated nuclear deformation leads to genome instability during epithelial-mesenchymal transition.

doi: 10.1016/j.isci.2023.106992

Figure Lengend Snippet: Figure 1. TGFb induces nuclear deformation, concomitant with defective nuclear lamina (A) Representative time-lapse images of A549 cells expressing GFP-H2B in the presence (+) or absence () of TGFb are shown. The alteration in the nuclear morphology became more evident 15 h after TGFb stimulation. The arrowheads indicate irregular nuclear shapes. Scale bars, 10 mm. (B) A549 cells were treated with (+) or without () TGFb for 72 h and stained for lamin A (green) and DNA (blue). The insets show a normal nucleus and two deformed nuclei classified as crumpling (a) or lobulation (b). Scale bars, 20 mm. (C) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The percentage of the cells with a crumpled or lobulated nucleus was measured (n R 339). (D) A549 cells were treated with TGFb for various times as indicated and stained for lamin A and DNA. The nuclear circularity (4p x area/perimeter2) of the cells was determined (n R 100). (E) Various cancer cell lines, as indicated, were treated with TGFb for 72 h and stained for lamin A and DNA. The percentage of the cells with a deformed nucleus was measured (n R 600). (F) A549 cells were treated with or without TGFb for 72 h and stained for lamin A. The line-scan profiles of lamin A at the selected region (as indicated by dashed lines) are shown using Zeiss Zen2 software. Scale bars, 10 mm. The percentage of the cells with apparent nucleoplasmic distribution of lamin A was measured (n R 246). (G) A549 cells were treated with or without TGFb for 72 h and solubilized in 1% NP40 lysis buffer. An equal proportion of cell lysates from the soluble and insoluble fractions was analyzed by immunoblotting with the antibodies as indicated. The

Article Snippet: Plasmid: HA-Smad2 Addgene Cat#11734 Plasmid: FLAG-Smad3 Addgene Cat#11742 Plasmid: HA-AKT2 Addgene Cat#16000 Plasmid: HA-AKT1 Dr. Chi-Ying Huang (National YangMing Chiao Tung University) N/A Plasmid: FLAG-lamin A This paper N/A Plasmid: pMD.G National RNAi Core Facility (Academia Sinica) https://rnai.genmed.sinica.edu.tw/vector.html Plasmid: pCMV-DR8.91 National RNAi Core Facility (Academia Sinica) https://rnai.genmed.sinica.edu.tw/vector.html Plasmid: pLKO-AS1-puro National RNAi Core Facility (Academia Sinica) https://rnai.genmed.sinica.edu.tw/vector.html Software and algorithms ZEN2 software Carl Zeiss N/A Odyssey CLx Imaging System LI-COR Biosciences N/A Image J NIH https://imagej.nih.gov/ij/ EXCEL Microsoft N/A GraphPad Prism GraphPad https://www.graphpad.com/features Photoshop CS6 Adobe N/A Illustrator CS6 Adobe N/A

Techniques: Expressing, Staining, Software, Lysis, Western Blot

Journal: eLife

Article Title: Neural control of growth and size in the axolotl limb regenerate

doi: 10.7554/eLife.68584

Figure Lengend Snippet:

Article Snippet: Software, algorithm , ZenPro software , Zeiss , 410138-1104-260 , .

Techniques: In Situ, Software, Plasmid Preparation, Modification