microplate reader Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher microplate reader microplate reader
    Microplate Reader Microplate Reader, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microplate reader microplate reader/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    microplate reader microplate reader - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    92
    BioTek Instruments microplate reader microplate reader
    High lytic activity of ClyR. ( A ) Time-kill curves of ClyR against S. dysgalactiae ATCC 35666. Bacterial cells were washed once with PBS, treated with 25 μg/ml ClyR, and the change of OD 600 (right Y-axis) were monitored by a <t>microplate</t> reader at 37 °C for 30 min. Triangles: ClyR; Circles: PBS controls. In parallel, the viable cell numbers (squares, left Y-axis) were calculated by plating onto BHI agar plates at different time points. ( B ) Dose-dependent lytic efficacy of ClyR against S. agalactiae S12, S. dysgalactiae ATCC 35666, or an equal mixture of both strains in market pasteurized milk at 30 °C for 1 h. ( C ) TEM images of S. dysgalactiae ATCC 35666 cells exposed to ClyR. Bar sizes: 500 nm. ( D ) Time-killing efficacy of ClyR (40 μg/ml) against S. agalactiae S12, S. dysgalactiae ATCC 35666, or an equal mixture of both strains in market pasteurized milk at 30 °C. ( E ) Lytic efficacy of ClyR (40 μg/ml) in pasteurized cow’s milk against S. agalactiae S12, or S. dysgalactiae ATCC 35666 at 30 °C for 1 h. Fresh cow milk samples A, B, and C were taken from healthy cows, and samples D and E were from mastitic cows. ( F ) Comparison of the activity of 0.89 μM ClyR or PlyCAC against various strains. ( G ) Comparison of the activity of 0.89 μM ClyR or PlyGBS-180 against S. dysgalactiae ATCC 35666. ( H ) Efficacy of PlyGBS-180 against S. dysgalactiae ATCC 35666 in market pasteurized milk at 30 °C for 60 min.
    Microplate Reader Microplate Reader, supplied by BioTek Instruments, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microplate reader microplate reader/product/BioTek Instruments
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    microplate reader microplate reader - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    99
    Bio-Rad imarktm microplate absorbance reader microplate reader
    Figure 11. ) per manufacturer’s protocol. Optical densities at 492 mm were measured using an <t>iMark</t> <t>Microplate</t> Reader.
    Imarktm Microplate Absorbance Reader Microplate Reader, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1771 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imarktm microplate absorbance reader microplate reader/product/Bio-Rad
    Average 99 stars, based on 1771 article reviews
    Price from $9.99 to $1999.99
    imarktm microplate absorbance reader microplate reader - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad microplate reader
    Recombinant expression of AvBD2 protein and antiviral activity of AvBD2 against NDV. (A) 12% SDS–PAGE analysis of His-tagged recombinant AvBD2 protein expressed in E . coli BL21 (DE3) cells. Lane 1: Supernatant; lane 2: Inclusion body; lane 3: Total protein of Rosetta containing AvBD2 without IPTG induction; lanes 4–6: Total protein of Rosetta containing AvBD2 at 2, 4, 6 h after induction with IPTG, respectively; lane 7: Purified recombinant AvBD2 protein; lane M: Protein marker. (B) 12% SDS–PAGE analysis of His-tagged AvBD2 protein expressed in Expi293 cells. Lane 1: purified AvBD2 with a His-tag on the C-terminal; lane 2: purified vector with a His-tag on the C-terminal; lane M: protein molecular weight marker. (C) Protection of CEFs from NDV infection by using the CCK-8 assay. Red curve , NDV (1 MOI) and AvBD2 protein (3.75–120 ng/μl) were mixed for 1.5 h at 37°C. Cells were washed and then incubated with the NDV-AvBD2 protein mixture for another 1.5 h at 37°C. Green curve , NDV (1 MOI) and equal volume of PB were mixed and then incubated with CEFs for 1.5 h at 37°C. Cells were washed and incubated with AvBD2 protein and equal volume of DMEM without serum for another 1.5 h. Purple curve , AvBD2 protein (3.75–120 ng/μl) and equal volume of DMEM without serum were mixed to incubate with CEFs for 1.5 h at 37°C. Cells were washed and then the mixtures of NDV (1 MOI) and equal volume of PB were added and incubated with CEFs for another 1.5 h at 37°C. Then, the cells were washed, and 2% DMEM were added and incubated for 48 h at 37°C. Then CCK-8 solution (5 mg/ml, 10 μl/well) was added to the plate. The absorbance at 450 nm was measured using a <t>microplate</t> reader after the incubation at 37°C for 1.5 h. The vector was used as control for each group. Cells without virus-protein mixture were also used as a negative control. Cells only infected with the virus served as a positive control. The experiment was performed in triplicate.
    Microplate Reader, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 39723 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microplate reader/product/Bio-Rad
    Average 99 stars, based on 39723 article reviews
    Price from $9.99 to $1999.99
    microplate reader - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    94
    Bruker Corporation microplate reader
    Recombinant expression of AvBD2 protein and antiviral activity of AvBD2 against NDV. (A) 12% SDS–PAGE analysis of His-tagged recombinant AvBD2 protein expressed in E . coli BL21 (DE3) cells. Lane 1: Supernatant; lane 2: Inclusion body; lane 3: Total protein of Rosetta containing AvBD2 without IPTG induction; lanes 4–6: Total protein of Rosetta containing AvBD2 at 2, 4, 6 h after induction with IPTG, respectively; lane 7: Purified recombinant AvBD2 protein; lane M: Protein marker. (B) 12% SDS–PAGE analysis of His-tagged AvBD2 protein expressed in Expi293 cells. Lane 1: purified AvBD2 with a His-tag on the C-terminal; lane 2: purified vector with a His-tag on the C-terminal; lane M: protein molecular weight marker. (C) Protection of CEFs from NDV infection by using the CCK-8 assay. Red curve , NDV (1 MOI) and AvBD2 protein (3.75–120 ng/μl) were mixed for 1.5 h at 37°C. Cells were washed and then incubated with the NDV-AvBD2 protein mixture for another 1.5 h at 37°C. Green curve , NDV (1 MOI) and equal volume of PB were mixed and then incubated with CEFs for 1.5 h at 37°C. Cells were washed and incubated with AvBD2 protein and equal volume of DMEM without serum for another 1.5 h. Purple curve , AvBD2 protein (3.75–120 ng/μl) and equal volume of DMEM without serum were mixed to incubate with CEFs for 1.5 h at 37°C. Cells were washed and then the mixtures of NDV (1 MOI) and equal volume of PB were added and incubated with CEFs for another 1.5 h at 37°C. Then, the cells were washed, and 2% DMEM were added and incubated for 48 h at 37°C. Then CCK-8 solution (5 mg/ml, 10 μl/well) was added to the plate. The absorbance at 450 nm was measured using a <t>microplate</t> reader after the incubation at 37°C for 1.5 h. The vector was used as control for each group. Cells without virus-protein mixture were also used as a negative control. Cells only infected with the virus served as a positive control. The experiment was performed in triplicate.
    Microplate Reader, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microplate reader/product/Bruker Corporation
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microplate reader - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    92
    Molecular Devices LLC microplate reader versamax microplate reader
    Recombinant expression of AvBD2 protein and antiviral activity of AvBD2 against NDV. (A) 12% SDS–PAGE analysis of His-tagged recombinant AvBD2 protein expressed in E . coli BL21 (DE3) cells. Lane 1: Supernatant; lane 2: Inclusion body; lane 3: Total protein of Rosetta containing AvBD2 without IPTG induction; lanes 4–6: Total protein of Rosetta containing AvBD2 at 2, 4, 6 h after induction with IPTG, respectively; lane 7: Purified recombinant AvBD2 protein; lane M: Protein marker. (B) 12% SDS–PAGE analysis of His-tagged AvBD2 protein expressed in Expi293 cells. Lane 1: purified AvBD2 with a His-tag on the C-terminal; lane 2: purified vector with a His-tag on the C-terminal; lane M: protein molecular weight marker. (C) Protection of CEFs from NDV infection by using the CCK-8 assay. Red curve , NDV (1 MOI) and AvBD2 protein (3.75–120 ng/μl) were mixed for 1.5 h at 37°C. Cells were washed and then incubated with the NDV-AvBD2 protein mixture for another 1.5 h at 37°C. Green curve , NDV (1 MOI) and equal volume of PB were mixed and then incubated with CEFs for 1.5 h at 37°C. Cells were washed and incubated with AvBD2 protein and equal volume of DMEM without serum for another 1.5 h. Purple curve , AvBD2 protein (3.75–120 ng/μl) and equal volume of DMEM without serum were mixed to incubate with CEFs for 1.5 h at 37°C. Cells were washed and then the mixtures of NDV (1 MOI) and equal volume of PB were added and incubated with CEFs for another 1.5 h at 37°C. Then, the cells were washed, and 2% DMEM were added and incubated for 48 h at 37°C. Then CCK-8 solution (5 mg/ml, 10 μl/well) was added to the plate. The absorbance at 450 nm was measured using a <t>microplate</t> reader after the incubation at 37°C for 1.5 h. The vector was used as control for each group. Cells without virus-protein mixture were also used as a negative control. Cells only infected with the virus served as a positive control. The experiment was performed in triplicate.
    Microplate Reader Versamax Microplate Reader, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microplate reader versamax microplate reader/product/Molecular Devices LLC
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    microplate reader versamax microplate reader - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    High lytic activity of ClyR. ( A ) Time-kill curves of ClyR against S. dysgalactiae ATCC 35666. Bacterial cells were washed once with PBS, treated with 25 μg/ml ClyR, and the change of OD 600 (right Y-axis) were monitored by a microplate reader at 37 °C for 30 min. Triangles: ClyR; Circles: PBS controls. In parallel, the viable cell numbers (squares, left Y-axis) were calculated by plating onto BHI agar plates at different time points. ( B ) Dose-dependent lytic efficacy of ClyR against S. agalactiae S12, S. dysgalactiae ATCC 35666, or an equal mixture of both strains in market pasteurized milk at 30 °C for 1 h. ( C ) TEM images of S. dysgalactiae ATCC 35666 cells exposed to ClyR. Bar sizes: 500 nm. ( D ) Time-killing efficacy of ClyR (40 μg/ml) against S. agalactiae S12, S. dysgalactiae ATCC 35666, or an equal mixture of both strains in market pasteurized milk at 30 °C. ( E ) Lytic efficacy of ClyR (40 μg/ml) in pasteurized cow’s milk against S. agalactiae S12, or S. dysgalactiae ATCC 35666 at 30 °C for 1 h. Fresh cow milk samples A, B, and C were taken from healthy cows, and samples D and E were from mastitic cows. ( F ) Comparison of the activity of 0.89 μM ClyR or PlyCAC against various strains. ( G ) Comparison of the activity of 0.89 μM ClyR or PlyGBS-180 against S. dysgalactiae ATCC 35666. ( H ) Efficacy of PlyGBS-180 against S. dysgalactiae ATCC 35666 in market pasteurized milk at 30 °C for 60 min.

    Journal: Scientific Reports

    Article Title: A chimeolysin with extended-spectrum streptococcal host range found by an induced lysis-based rapid screening method

    doi: 10.1038/srep17257

    Figure Lengend Snippet: High lytic activity of ClyR. ( A ) Time-kill curves of ClyR against S. dysgalactiae ATCC 35666. Bacterial cells were washed once with PBS, treated with 25 μg/ml ClyR, and the change of OD 600 (right Y-axis) were monitored by a microplate reader at 37 °C for 30 min. Triangles: ClyR; Circles: PBS controls. In parallel, the viable cell numbers (squares, left Y-axis) were calculated by plating onto BHI agar plates at different time points. ( B ) Dose-dependent lytic efficacy of ClyR against S. agalactiae S12, S. dysgalactiae ATCC 35666, or an equal mixture of both strains in market pasteurized milk at 30 °C for 1 h. ( C ) TEM images of S. dysgalactiae ATCC 35666 cells exposed to ClyR. Bar sizes: 500 nm. ( D ) Time-killing efficacy of ClyR (40 μg/ml) against S. agalactiae S12, S. dysgalactiae ATCC 35666, or an equal mixture of both strains in market pasteurized milk at 30 °C. ( E ) Lytic efficacy of ClyR (40 μg/ml) in pasteurized cow’s milk against S. agalactiae S12, or S. dysgalactiae ATCC 35666 at 30 °C for 1 h. Fresh cow milk samples A, B, and C were taken from healthy cows, and samples D and E were from mastitic cows. ( F ) Comparison of the activity of 0.89 μM ClyR or PlyCAC against various strains. ( G ) Comparison of the activity of 0.89 μM ClyR or PlyGBS-180 against S. dysgalactiae ATCC 35666. ( H ) Efficacy of PlyGBS-180 against S. dysgalactiae ATCC 35666 in market pasteurized milk at 30 °C for 60 min.

    Article Snippet: The relative light units (RLUs) were monitored by the microplate reader in 5-sec integrations.

    Techniques: Activity Assay, Transmission Electron Microscopy

    Figure 11. ) per manufacturer’s protocol. Optical densities at 492 mm were measured using an iMark Microplate Reader.

    Journal: Human Vaccines & Immunotherapeutics

    Article Title: Synergistic effect of methionine encephalin (MENK) combined with pidotimod(PTD) on the maturation of murine dendritic cells (DCs)

    doi: 10.4161/hv.23137

    Figure Lengend Snippet: Figure 11. ) per manufacturer’s protocol. Optical densities at 492 mm were measured using an iMark Microplate Reader.

    Article Snippet: Readings were made at 450 nm with correction at 570 nm, on the iMark Microplate Reader (BIO-RAD).

    Techniques:

    Effects of PD1–PD6 on adipocyte differentiation of 3T3-L1 cells. The 3T3-L1 cells were exposed to 10% FBS/DMEM containing 10 μg/mL insulin and 1 μM dexamethasone in the presence of various concentrations (1, 10, or 20 μΜ) of PD1 – PD6 or rosiglitazone (1 μΜ). After eight days, treated cells were stained with Oil red O for morphological assessment by microscopy ( A ) and lipid contents was determined using an iMark Microplate Absorbance Reader ( B ). The results shown are representative of three independent experiments, and are presented as means ± SDs ( n = 3). * p

    Journal: Marine Drugs

    Article Title: Synthesis of Phthalimide Derivatives as Potential PPAR-γ Ligands

    doi: 10.3390/md14060112

    Figure Lengend Snippet: Effects of PD1–PD6 on adipocyte differentiation of 3T3-L1 cells. The 3T3-L1 cells were exposed to 10% FBS/DMEM containing 10 μg/mL insulin and 1 μM dexamethasone in the presence of various concentrations (1, 10, or 20 μΜ) of PD1 – PD6 or rosiglitazone (1 μΜ). After eight days, treated cells were stained with Oil red O for morphological assessment by microscopy ( A ) and lipid contents was determined using an iMark Microplate Absorbance Reader ( B ). The results shown are representative of three independent experiments, and are presented as means ± SDs ( n = 3). * p

    Article Snippet: Oil red O was eluted by adding 200 μL of isopropanol on a rotary shaker for 5 min. Eluents were transferred to a 96-well plate and ODs were measured using a iMark Microplate Absorbance Reader (Bio-Rad Laboratories, Hercules, CA, USA) at 544 nm.

    Techniques: Staining, Microscopy, Microplate Reader Absorbance Measurement

    Recombinant expression of AvBD2 protein and antiviral activity of AvBD2 against NDV. (A) 12% SDS–PAGE analysis of His-tagged recombinant AvBD2 protein expressed in E . coli BL21 (DE3) cells. Lane 1: Supernatant; lane 2: Inclusion body; lane 3: Total protein of Rosetta containing AvBD2 without IPTG induction; lanes 4–6: Total protein of Rosetta containing AvBD2 at 2, 4, 6 h after induction with IPTG, respectively; lane 7: Purified recombinant AvBD2 protein; lane M: Protein marker. (B) 12% SDS–PAGE analysis of His-tagged AvBD2 protein expressed in Expi293 cells. Lane 1: purified AvBD2 with a His-tag on the C-terminal; lane 2: purified vector with a His-tag on the C-terminal; lane M: protein molecular weight marker. (C) Protection of CEFs from NDV infection by using the CCK-8 assay. Red curve , NDV (1 MOI) and AvBD2 protein (3.75–120 ng/μl) were mixed for 1.5 h at 37°C. Cells were washed and then incubated with the NDV-AvBD2 protein mixture for another 1.5 h at 37°C. Green curve , NDV (1 MOI) and equal volume of PB were mixed and then incubated with CEFs for 1.5 h at 37°C. Cells were washed and incubated with AvBD2 protein and equal volume of DMEM without serum for another 1.5 h. Purple curve , AvBD2 protein (3.75–120 ng/μl) and equal volume of DMEM without serum were mixed to incubate with CEFs for 1.5 h at 37°C. Cells were washed and then the mixtures of NDV (1 MOI) and equal volume of PB were added and incubated with CEFs for another 1.5 h at 37°C. Then, the cells were washed, and 2% DMEM were added and incubated for 48 h at 37°C. Then CCK-8 solution (5 mg/ml, 10 μl/well) was added to the plate. The absorbance at 450 nm was measured using a microplate reader after the incubation at 37°C for 1.5 h. The vector was used as control for each group. Cells without virus-protein mixture were also used as a negative control. Cells only infected with the virus served as a positive control. The experiment was performed in triplicate.

    Journal: Frontiers in Microbiology

    Article Title: Induction of Avian β-Defensin 2 Is Possibly Mediated by the p38 MAPK Signal Pathway in Chicken Embryo Fibroblasts After Newcastle Disease Virus Infection

    doi: 10.3389/fmicb.2018.00751

    Figure Lengend Snippet: Recombinant expression of AvBD2 protein and antiviral activity of AvBD2 against NDV. (A) 12% SDS–PAGE analysis of His-tagged recombinant AvBD2 protein expressed in E . coli BL21 (DE3) cells. Lane 1: Supernatant; lane 2: Inclusion body; lane 3: Total protein of Rosetta containing AvBD2 without IPTG induction; lanes 4–6: Total protein of Rosetta containing AvBD2 at 2, 4, 6 h after induction with IPTG, respectively; lane 7: Purified recombinant AvBD2 protein; lane M: Protein marker. (B) 12% SDS–PAGE analysis of His-tagged AvBD2 protein expressed in Expi293 cells. Lane 1: purified AvBD2 with a His-tag on the C-terminal; lane 2: purified vector with a His-tag on the C-terminal; lane M: protein molecular weight marker. (C) Protection of CEFs from NDV infection by using the CCK-8 assay. Red curve , NDV (1 MOI) and AvBD2 protein (3.75–120 ng/μl) were mixed for 1.5 h at 37°C. Cells were washed and then incubated with the NDV-AvBD2 protein mixture for another 1.5 h at 37°C. Green curve , NDV (1 MOI) and equal volume of PB were mixed and then incubated with CEFs for 1.5 h at 37°C. Cells were washed and incubated with AvBD2 protein and equal volume of DMEM without serum for another 1.5 h. Purple curve , AvBD2 protein (3.75–120 ng/μl) and equal volume of DMEM without serum were mixed to incubate with CEFs for 1.5 h at 37°C. Cells were washed and then the mixtures of NDV (1 MOI) and equal volume of PB were added and incubated with CEFs for another 1.5 h at 37°C. Then, the cells were washed, and 2% DMEM were added and incubated for 48 h at 37°C. Then CCK-8 solution (5 mg/ml, 10 μl/well) was added to the plate. The absorbance at 450 nm was measured using a microplate reader after the incubation at 37°C for 1.5 h. The vector was used as control for each group. Cells without virus-protein mixture were also used as a negative control. Cells only infected with the virus served as a positive control. The experiment was performed in triplicate.

    Article Snippet: In brief, CEFs in different multiplicity of infection (MOI) (0.01, 0.1, 1, 10, and 100) at different time points (24, 36, and 48 h post-infection, hpi) was added to the CCK-8 solution (5 mg/ml, 10 μl/well) before incubation at 37°C for 1 h. Absorbance was then measured at a wavelength of 450 nm by using a microplate reader (model 680; Bio-Rad Laboratories, Hercules, CA, United States).

    Techniques: Recombinant, Expressing, Activity Assay, SDS Page, Purification, Marker, Plasmid Preparation, Molecular Weight, Infection, CCK-8 Assay, Incubation, Negative Control, Positive Control

    Biological characterization of NDV-F48E9 infection in CEFs. (A) Survival rate of NDV infected CEFs in different MOI at different time points. CCK-8 was used to detect cell viability. Absorbance was measured at a wavelength of 450 nm by using a microplate reader (model 680; Bio-Rad Laboratories, Hercules, CA, United States). Protective rate was described as previous reports ( Pauwels et al., 1988 ; Gong et al., 2010 ). Each group has three replicates. The difference between the same MOI at three different infection time points is represented by ∗ p

    Journal: Frontiers in Microbiology

    Article Title: Induction of Avian β-Defensin 2 Is Possibly Mediated by the p38 MAPK Signal Pathway in Chicken Embryo Fibroblasts After Newcastle Disease Virus Infection

    doi: 10.3389/fmicb.2018.00751

    Figure Lengend Snippet: Biological characterization of NDV-F48E9 infection in CEFs. (A) Survival rate of NDV infected CEFs in different MOI at different time points. CCK-8 was used to detect cell viability. Absorbance was measured at a wavelength of 450 nm by using a microplate reader (model 680; Bio-Rad Laboratories, Hercules, CA, United States). Protective rate was described as previous reports ( Pauwels et al., 1988 ; Gong et al., 2010 ). Each group has three replicates. The difference between the same MOI at three different infection time points is represented by ∗ p

    Article Snippet: In brief, CEFs in different multiplicity of infection (MOI) (0.01, 0.1, 1, 10, and 100) at different time points (24, 36, and 48 h post-infection, hpi) was added to the CCK-8 solution (5 mg/ml, 10 μl/well) before incubation at 37°C for 1 h. Absorbance was then measured at a wavelength of 450 nm by using a microplate reader (model 680; Bio-Rad Laboratories, Hercules, CA, United States).

    Techniques: Infection, CCK-8 Assay