Journal: Journal of visualized experiments : JoVE

Article Title: Murine Model of Controlled Cortical Impact for the Induction of Traumatic Brain Injury

doi: 10.3791/60027

Figure Lengend Snippet: Materials

Article Snippet: High Speed Rotary Micromotor KiT0 , Foredom Electric Company , K.1070 , .

Techniques: Injection, Sterility

Journal: Advanced Drug Delivery Reviews

Article Title: Orally ingestible medical devices for gut engineering

doi: 10.1016/j.addr.2020.05.004

Figure Lengend Snippet: Examples of passive and active microdevices for oral drug delivery and as advanced in vitro cell model. 3A) Pharmacokinetic profile showing the enhanced in vivo bioavailability of acyclovir using planar microdevices (inset) compared to an oral solution with the same concentration of acyclovir. Reprinted from with permission. 3B) Schematic of the printing process of bottom-up fabricated enteric devices for oral delivery. The polymer dispersion was ejected onto the silicon wafer by a picoliter dispenser, where after evaporation of the solvent forms the device body. The same dispenser was used to print drug formulation into each device and a second polymer was applied on top to seal the device before removal from the silicon wafer. Adapted from with permission. 3C) Human gut-on-a-chip microdevice. (i) Photograph of the microdevice, where blue and red dyes fill the upper and lower channels, respectively. (ii) Cross-sectional schematic of the device showing how suction to side channels (grey arrows) applies peristalsis-like mechanical constrictions and fluid flow (white arrows) generates shear stress. (iii) Micrograph showing intestinal basal crypt (red arrow) and villi (white arrow) formed by human Caco-2 cells grown for ~100 h in the microdevice. (iv) Confocal immunofluorescence image showing a horizontal cross-section of intestinal villi similar to the ones shown in (iii). Scale bars represent 50 μm unless indicated otherwise on C. Reprinted from with permission. 3D) Self-propelled microrockets for targeted drug delivery in the stomach. (i) SEM image of a full DOX/poly (aspartic acid)/Fe-Zn microrocket and (ii) energydispersive X-ray spectroscopy mappings of Zn inside the microrocket. (iii) Superimposed fluorescent images of the whole stomachs of mice collected 30 min after administration of ultrapure water and (iv) DOX/poly (aspartic acid)/Pt microrockets and (v) DOX/poly (aspartic acid)/Fe-Zn microrockets. (vi) Histological evaluation of gastric tissue 24 h after administration of poly (aspartic acid)/Fe-Zn microrockets and (vii) water. Reprinted with permission from Zhou et al., Self-propelled and targeted drug delivery of poly(aspartic acid)/iron-zinc microrocket in the stomach, ACS Nano, 13. Copyright 2019 American Chemical Society. 3E) Biomimetic micromotors for delivery of antigens for oral vaccination. (i) Schematic of the formulation concept; after oral ingestion of micromotors, the coating is dissolved in the small intestine, which activates the motor to provide enhanced retention and stimulation. (ii) Images of the GI tract of mice 6 h after oral administration of labeled static microparticles or (iii) micromotors. (iv) Data showing a significantly higher level of IgA titers against α-toxin after administration of micromotors compared to static microparticles. Reprinted with permission from Wei et al., Biomimetic micromotor enables active delivery of antigens for oral vaccination, Nano Letters, 19. Copyright 2019 American Chemical Society. 3F) Micromotors for local treatment of stomach infection in vivo . (i) Schematic of the preparation of micromotors; a dispersion of Mg microparticles is dispersed on a glass slide followed by TiO 2 atomic layer deposition and coating with drug-loaded PLGA and chitosan. (ii) Time-lapse images of the propulsion of the micromotors after 2, 4 and 6 min in simulated gastric fluid (pH ~1.3). (iii) Retention of the micromotors visualized with bright-field and fluorescence overlay images of freshly removed mouse stomachs 0 h after oral gavage of ultrapure water as control and 2 h after oral gavage of micromotors. Shared under a Creative Commons Attribution 4.0 International License with copyrights reserved with the authors . (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Reprinted with permission from Wei et al., Biomimetic micromotor enables active delivery of antigens for oral vaccination, Nano Letters, 19.

Techniques: In Vitro, In Vivo, Concentration Assay, Polymer, Dispersion, Evaporation, Solvent, Drug Formulation, Shear, Immunofluorescence, Spectroscopy, Formulation, Labeling, Infection, Fluorescence, Control

Journal: Advanced Drug Delivery Reviews

Article Title: Orally ingestible medical devices for gut engineering

doi: 10.1016/j.addr.2020.05.004

Figure Lengend Snippet: Material composition and biocompatibility of micro- and macro-scale ingestible devices for GI manipulation discussed in the present review.

Article Snippet: Reprinted with permission from Wei et al., Biomimetic micromotor enables active delivery of antigens for oral vaccination, Nano Letters, 19.

Techniques: Capsules, MTT Assay, In Vitro, Ex Vivo, In Vivo, Papanicolaou Stain, Vaccines, Infection, Injection, Membrane, Sampling