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Image Search Results
Journal: Cells
Article Title: Recapitulation of Retinal Damage in Zebrafish Larvae Infected with Zika Virus
doi: 10.3390/cells11091457
Figure Lengend Snippet: ZIKV infects one-cell stage embryo. The infection ( n = 98) was performed by microinjecting embryos at 0 hpf with approximately 2 or 3 nL of ZIKV BR (1 × 10 7 PFU/mL, 2 a.p.) utilizing a M205C stereomicroscope coupled with an Injectman ® 4 microinjector. Another group ( n = 83) was left without injection and formed the negative control group. Embryos were incubated in E2 0.5× medium at 28 °C, and viral RNA extracted from entire larvae was used for measurements via real-time quantitative PCR (qRT-PCR) after 3, 6, 12, 24, 48, and 72 hpf of the Zika viral load ( A ). Then, 72 hpf-infected larvae were sampled for qRT-PCR analysis of ( B ) IFNφ1, IFNφ3, and IFNφ4. cDNA was used in qRT-PCR reaction using primers specific for zebrafish, IL-1, TNF, IL-6, IL-8, and IL-34 ( C ), and iNOSa, iNOSb, NOX1, and NOX2 ( D ). The relative expression was normalized to the expression of EF-1a or GAPDH, and it is expressed as fold induction relative to the expression level in the control group (dotted line). For the analysis of gene expression, genes with fold change ≥1.5 were considered differentially expressed. ZIKV infection led to 14 and 16% of mortality ( E ), and a reduction of 17% of the head size was observed in ZIKV-injected larvae ( F ). Each bar represents the mean ± SEM. * p < 0.05 compared with the negative control group.
Article Snippet: Embryos at 0 hpf with 1 cell (one-cell stage embryo) mounted in the grooves of an agarose-coated plate (#16500100, Invitrogen, Carlsbad, CA, USA, EUA) were injected using an M205C stereomicroscope with ZIKV BR samples (1 × 10 7 PFU/mL) using a microneedle (#5242952008 femtotips 930000043 with 0.5–0.7 μm Eppendorf, Hamburg, Germany) coupled with an Injectman ® 4 pneumatic
Techniques: Infection, Injection, Negative Control, Incubation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing