Journal: Life Science Alliance

Article Title: Themis controls T cell activation, effector functions, and metabolism of peripheral CD8 + T cells

doi: 10.26508/lsa.202302156

Figure Lengend Snippet: (A) Schematic layout of the microfluidic device to perform 3D co-culture of B16 melanoma cells and T cells from Themis cWT or cKO OT-I mice treated with tamoxifen (TX). The central channel (in purple) represents the tumor region, whereas the lateral channels (in cyan) provide nutrients and allow for CTL injection. (B) Zoomed-in schematic of the microfluidic system. Target melanoma cells were embedded in an extracellular matrix-like hydrogel and inserted in the central microchannel flanked by two lateral channels containing cell culture medium and CTL. Live and dead B16 melanoma cells are shown in green and red, respectively. CTL are shown in blue. (C) Scatter plot of the percentage of dead B16–OVA cells after 24 h from the injection of OT-I Themis cWT or cKO TX CTL in the fluidic microchannel. **** P < 0.0001. (D) Representative images of the 3D tumor region after 24 h from the injection of CTL (in blue) into the system. Live and dead B16–OVA cells are shown in green and red, respectively. (E) Scatter plot of the percentage of dead B16-Q4R7 cells after 24 h from the injection of OT-I Themis cWT or cKO TX CTL in the fluidic microchannel. * P = 0.0231, **** P < 0.0001. (F) Representative images of the 3D tumor region after 24 h from the injection of CTL (in blue) into the system. Live and dead B16-Q4R7 cells are shown in green and red, respectively. (G) Scatter plot of the percentage of dead B16-OVA and B16-Q4R7 cells after 24 h from the injection of OT-I Themis cWT CTL in the fluidic microchannel. **** P < 0.0001. (H) Scatter plot of the percentage of dead B16-OVA and B16-Q4R7 cells after 24 h from the injection of OT-I Themis cKO TX CTL in the fluidic microchannel. **** P < 0.0001. (I) Scatter plot of the number of OT-I Themis cWT and cKO TX CTL infiltrated after 24 h in the 3D tumor region with B16-OVA. * P = 0.0369. (J) Scatter plot of the number of OT-I Themis cWT and cKO TX CTL infiltrated after 24 h in the 3D tumor region with B16-Q4R7. ** P = 0.0019. Mean ± SD values are shown. Each data point in the graphs represent the value for one region of interest. Data are from n = 5 regions of interest per device, n = 3 devices per condition and n = 2 independent experiments. Samples were compared by one-way ANOVA with multiple comparisons (C, E) and by unpaired two-tailed t test (G, H, I, J).

Article Snippet: Collagen solution was prepared and inserted in microfluidic devices (AIM Biotech) following a previously described protocol ( ).

Techniques: Co-Culture Assay, Injection, Cell Culture, Two Tailed Test

Journal: Nature Communications

Article Title: Ionizable polymeric micelles (IPMs) for efficient siRNA delivery

doi: 10.1038/s41467-024-55721-w

Figure Lengend Snippet: Three IOs with PEG-PLA were prepared by microfluidic chip to obtain IPM. si HSP47 and si HMGB1 were delivered to treat hepatic fibrosis by targeting activated hepatic stellate cells with the addition of FAPi-PEG-PLA in the formulation. Meanwhile, attenuated ABC phenomenon of IPM was observed.

Article Snippet: The aqueous phase and organic phase were mixed at a flow rate of 3:1 using a microfluidic device (NEXSTAR NANO2, C10-1).

Techniques: Formulation