Journal: Dental materials : official publication of the Academy of Dental Materials

Article Title: Evaluation of Biofilm Formation on Novel Copper-Catalyzed Azide-Alkyne Cycloaddition (CuAAC)-Based Resins for Dental Restoratives

doi: 10.1016/j.dental.2018.01.011

Figure Lengend Snippet: Evaluation of bioburden on novel CuAAC-based and BisGMA-TEGDMA-based composites using non-disruptive (A) and disruptive analyses (B). Nondisruptive luciferase analysis (A) of S. mutans firefly biofilm grown on CuAAC 60% composite discs (containing AZ-2 diazide, trialkyne (a mole ratio of 1:1 to N3:alkyne), with 2 mole percentage of CuCl2[PMDETA] per functionality, 2 mole percentage of CQ and 60-weight percentage of alkyne-functionalized microfillers (Schott, 0.4 µm)) and on BisGMA-TEGDMA 60% composite discs (n=12) (containing BisGMA-TEGDMA (70:30 weight ratio) with 2-weight percentage of CQ having 60-weight percentage of methacrylated microfillers (Schott, 0.4 µm)) indicated a statistically significant reduction (p >0.05) in luminescence, indicating a reduced bioburden on CuAAC composite discs. Disruptive analysis (B) of CuAAC 60% composite discs and BisGMA-TEGDMA 60% composite discs also showed a significant reduction (p <0.05), having nearly a 2-log reduction in bioburden on CuAAC based composites.

Article Snippet: Nondisruptive luciferase analysis (A) of S. mutans firefly biofilm grown on CuAAC 60% composite discs (containing AZ-2 diazide, trialkyne (a mole ratio of 1:1 to N 3 :alkyne), with 2 mole percentage of CuCl 2 [PMDETA] per functionality, 2 mole percentage of CQ and 60-weight percentage of alkyne-functionalized microfillers (Schott, 0.4 µm)) and on BisGMA-TEGDMA 60% composite discs (n=12) (containing BisGMA-TEGDMA (70:30 weight ratio) with 2-weight percentage of CQ having 60-weight percentage of methacrylated microfillers (Schott, 0.4 µm)) indicated a statistically significant reduction (p >0.05) in luminescence, indicating a reduced bioburden on CuAAC composite discs.

Techniques: Bioburden Testing, Luciferase

Journal: PLoS ONE

Article Title: Intrahepatic Vascular Anatomy in Rats and Mice—Variations and Surgical Implications

doi: 10.1371/journal.pone.0141798

Figure Lengend Snippet: Comparison of previous anatomical studies and this study.

Article Snippet: Additional four portal veins were injected with Microfil ® (Flow Tech Inc., USA).

Techniques: Comparison, In Vivo, Ex Vivo, Dissection, Preserving, Functional Assay

Journal: Thrombosis and Haemostasis

Article Title: Endothelial β1 Integrin-Mediated Adaptation to Myocardial Ischemia

doi: 10.1055/s-0040-1721505

Figure Lengend Snippet: Growth of blood vessels into the infarcted myocardium, LV function, and cardiac survival after permanent LAD occlusion require endothelial Itgb1 expression . ( A–D, H–K ) Representative bright-field images of Microfil-perfused mouse hearts to visualize the coronary vasculature 14 days after permanent LAD occlusion. Mice were treated with (A, B) ctrl-AB and (C, D) β1-B-AB. Alternatively, tamoxifen-induced (H, I) control and (J, K) Itgb1 iECKO mice were used. ( E–G, L–N ) Quantification of LV myocardial function represented by (E, L) the end-diastolic volume, (F, M) the end-systolic volume, and (G, N) the LV ejection fraction at 0, 1, 7, and 14 days after LAD occlusion in wild-type mice treated with (E–G) ctrl-AB versus β1-B-AB or in (L–N) control versus Itgb1 iECKO mice. Reported values are means ± SEM. (E–G) Ctrl-AB: n = 5; β1-B-AB: n = 5; (L–N) control: n = 7; Itgb1 iECKO n = 5. Statistical significance was determined using unpaired two-tailed Student's t -test. * p -Values: (N) 7 d = 0.01; 14 d = 0.03. ( O–R ) Representative bright-field images of an Itgb1 iECKO mouse heart with myocardial rupture as (O, P) overview or as (Q, R) transversal sections. Arrows indicate the rupture. In panels (Q) and (R), the LV and RV are labeled. ( S ) Quantification of survival in a Kaplan–Meier curve in control versus Itgb1 iECKO mice. Control 0 d: n = 10, 7 d: n = 8; Itgb1 iECKO 0 d: n = 8, 1 d: n = 7, and upon 4 d: n = 5. Of note, one Itgb1 iECKO mouse died after 0 d measurement of cardiac function, but before permanent LAD occlusion, and therefore was excluded from the Kaplan–Meier curve. Only mice which received a permanent LAD occlusion are shown. Statistical significance was determined using the log-rank (Mantel–Cox) test. LAD, left anterior descending artery; LV, left ventricular; SEM, standard error of mean.

Article Snippet: To image the entire coronary vasculature, hearts were washed and retrograde-perfused via the thoracic aorta with Microfil compound (Flow Tech Inc.), previously described but modified.

Techniques: Expressing, Control, Two Tailed Test, Labeling