Journal: medRxiv

Article Title: Development and Validation of a Mobile Laboratory Workflows for Wastewater and Environmental Surveillance with Application in Sub Saharan Africa

doi: 10.64898/2026.04.01.26349919

Figure Lengend Snippet: Benchmarking of Multiplex PCR metabarcoding and ONT-16S Barcoding kit. ( A ) Microbe community composition obtained from ONT-16S (left panels) and Multiplex PCR metabarcoding (right panels) sequencing of ML-extracted (ML) and ZymoBIOMICS-extracted (ZP) samples. ( B ) Venn diagrams depicting unique and shared bacterial taxa across extraction methods and sequencing approaches. The diagram shows the percentage of bacterial features detected with >1 read in all three replicates for the two extraction methods: ML (mobile laboratory workflow) and ZP (ZymoBIOMICS kit). Left: Oxford Nanopore 16S kit; Right: library preparation using a multiplex primer set. ( C ) Venn diagrams of bacterial features shared between library preparation methods. Features with ≥1 read in all three replicates are shown for the 16S kit (Oxford Nanopore) and the Multiplex primer set. Left: Mobile laboratory (ML) protocol; Right: ZymoBIOMICS (ZP) protocol

Article Snippet: DNA was extracted from 24h composite wastewater samples (after prior filtration through 0.22 μm nitrocellulose membranes, WhatmanTM 25 mm) and the ZymoBIOMICS Microbial Community Standard (Zymo Research, USA) using the ML-NA protocol ( ).

Techniques: Multiplex Assay, Sequencing, Extraction

Journal: Applied and Environmental Microbiology

Article Title: Quantitative metagenomics using a portable protocol

doi: 10.1128/aem.02179-25

Figure Lengend Snippet: Evaluation of DNA extraction methods on the microbial community in mixed liquor sludge samples (ML). ( A ) Purity and quantity and ( B ) DNA fragment size distribution for DNA extracted using different protocols. The line, bar, and bubble plots indicate the average purity, DNA yield, and fragment distribution, respectively. The star, circle, and filled triangle represent the mean, median, and peak of DNA fragments by molarity, respectively. ( C ) Alpha diversity (Chao1 index), ( D ) beta diversity, and ( E ) composition of the microbial community for prokaryotes using 16S rRNA gene amplicon sequencing. Beta diversity is measured using an NMDS2 plot based on Bray-Curtis distance.

Article Snippet: DNA extracted from ZymoBIOMICS Microbial Community Standard (Even Distribution) (Zymo Even Cell, ) and gut microbiome standard (Zymo Gut, ) (Zymo Research Corporation, CA, USA), ML, SE, and AL using the optimized DNA extraction protocol (see “A portable DNA extraction strategy was validated across varying microbial loads and sample types,” below) was used as the sample DNA.

Techniques: DNA Extraction, Amplification, Sequencing

Journal: Animal Microbiome

Article Title: Multi-omics characterization of blood metabolites and cervical microbiota associated with estrus in simmental cattle

doi: 10.1186/s42523-026-00571-8

Figure Lengend Snippet: Bacterial community structure in the cervical microbiota. ( A ) OTU distribution. ( B ) α diversity indices. ( C ) PCoA clustering. ( D ) Dominant phyla ( Firmicutes , Bacteroidetes ). ( E ) Genus-level composition. ( F ) Clustered heatmap of the dominant genera. ( G ) LEfSe-identified bacterial biomarkers (LDA > 2). Data from 16 S rRNA sequencing ( n = 6/group)

Article Snippet: Genomic DNA was extracted from cervical swabs using the FastPure Microbiome DNA Isolation Kit (Vazyme, Nanjing, China) following the manufacturer’s instructions.

Techniques: Sequencing

Journal: Animal Microbiome

Article Title: Multi-omics characterization of blood metabolites and cervical microbiota associated with estrus in simmental cattle

doi: 10.1186/s42523-026-00571-8

Figure Lengend Snippet: Fungal community structure in the cervical microbiota. ( A ) OTU distribution. ( B ) α diversity indices. ( C ) PCoA clustering. ( D ) Dominant phyla ( Ascomycota , Basidiomycota ). ( E ) Genus-level composition. ( F ) Clustered heatmap of the dominant genera. ( G ) LEfSe-identified fungal biomarkers (LDA > 2). Data from ITS sequencing ( n = 6/group)

Article Snippet: Genomic DNA was extracted from cervical swabs using the FastPure Microbiome DNA Isolation Kit (Vazyme, Nanjing, China) following the manufacturer’s instructions.

Techniques: Sequencing

Journal: Animal Microbiome

Article Title: Multi-omics characterization of blood metabolites and cervical microbiota associated with estrus in simmental cattle

doi: 10.1186/s42523-026-00571-8

Figure Lengend Snippet: Correlations between reproductive hormones and cervical microbiota. ( A ) Heatmap showing Spearman correlations between serum hormone concentrations (E2, PROG) and relative abundances of bacterial phyla. ( B ) Correlations between hormones and fungal genera ( Bacteroides , Prevotellaceae _UCG-003, p < 0.05)

Article Snippet: Genomic DNA was extracted from cervical swabs using the FastPure Microbiome DNA Isolation Kit (Vazyme, Nanjing, China) following the manufacturer’s instructions.

Techniques: