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Image Search Results
Journal: Molecular Medicine Reports
Article Title: circRNA-0006896-miR1264-DNMT1 axis plays an important role in carotid plaque destabilization by regulating the behavior of endothelial cells in atherosclerosis
doi: 10.3892/mmr.2021.11950
Figure Lengend Snippet: Identification of serum-Exos. (A) Flow chart of exosome isolation and circRNA analysis. (B) Morphology of serum-Exos visualized under a transmission electron microscope. Scale bar, 2.0 µm (C) Western blot analysis of the exosome-specific markers TSG101, CD63 and CD9 in SA-Exos and UA-Exos. (D) Nanoparticle tracking analysis of exosome size distribution. (E) Diameter and (F) concentration of serum-Exos in the SA and UA groups. Data are presented as the mean ± SD. ***P<0.001, and *P<0.05. n=3 in each group. Serum-Exos, serum exosomes; circRNA, circular RNA; SA, stable plaque atherosclerosis; UA, unstable/vulnerable plaque atherosclerosis.
Article Snippet: The
Techniques: Isolation, Transmission Assay, Microscopy, Western Blot, Concentration Assay
Journal: Molecular Medicine Reports
Article Title: circRNA-0006896-miR1264-DNMT1 axis plays an important role in carotid plaque destabilization by regulating the behavior of endothelial cells in atherosclerosis
doi: 10.3892/mmr.2021.11950
Figure Lengend Snippet: circRNA microarray expression data in serum-Exos (A) Box plots displaying the variations in circRNA expression after normalization. (B) Volcano plot of circRNAs. The vertical lines correspond to 1.2-fold up and down, respectively, and the horizontal line represents the P-value cut-off of 0.05. Statistically significant differentially expressed circRNAs are represented by red symbols. (C) Scatter plot of circRNA expression. Symbols above the upper green line or below the lower green line represent circRNAs with a change in expression between the SA-Exos and US-Exos groups >1.2-fold. (D) Hierarchical clustering of circRNA expression across the SA-Exos and UA-Exos groups. Gene expression profiles are shown in rows. Red indicates upregulated expression of circRNAs, and green indicates downregulated expression of circRNAs. Exos, exosomes; circRNA, circular RNA; SA, stable plaque atherosclerosis; UA, unstable/vulnerable plaque atherosclerosis.
Article Snippet: The
Techniques: Microarray, Expressing, Gene Expression
Journal: Molecular Medicine Reports
Article Title: circRNA-0006896-miR1264-DNMT1 axis plays an important role in carotid plaque destabilization by regulating the behavior of endothelial cells in atherosclerosis
doi: 10.3892/mmr.2021.11950
Figure Lengend Snippet: Validation of expression patterns for circRNAs and construction of the ceRNA network. (A) RT-qPCR validation of the expression patterns of six circRNA candidates. (B) RT-qPCR validation of circRNA-0006896 expression in patients with SA or UA. (C) circRNA-0006896 ceRNA network diagram. Yellow diamonds represent the circRNAs, blue triangles correspond to the target miRNAs, and red circles represent miRNA-targeted mRNAs. RT-qPCR was performed in triplicate. Data are presented as the mean ± SD. ***P<0.001 and **P<0.01. Exos, exosomes; circRNA, circular RNA; miRNA, microRNA; SA, stable plaque atherosclerosis; UA, unstable/vulnerable plaque atherosclerosis; RT-qPCR, reverse transcription-quantitative PCR.
Article Snippet: The
Techniques: Biomarker Discovery, Expressing, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction
Journal: Molecular Medicine Reports
Article Title: circRNA-0006896-miR1264-DNMT1 axis plays an important role in carotid plaque destabilization by regulating the behavior of endothelial cells in atherosclerosis
doi: 10.3892/mmr.2021.11950
Figure Lengend Snippet: Correlation between circRNA-0006896 expression and biochemical parameters in AS patients with SA or UA.
Article Snippet: The
Techniques: Expressing
Journal: Molecular Medicine Reports
Article Title: circRNA-0006896-miR1264-DNMT1 axis plays an important role in carotid plaque destabilization by regulating the behavior of endothelial cells in atherosclerosis
doi: 10.3892/mmr.2021.11950
Figure Lengend Snippet: circRNA-006896-miR1264-DNMT1 axis in HUVECs. (A) RT-qPCR validation of the expression patterns of six circRNA-006896 targeted-miRNAs in SA-Exos or UA-Exos-treated HUVECs. (B) Predicted formation of duplexes at the miR1264 binding sites in circRNA-0006896 is indicated. (C) A dual-luciferase reporter gene assay was carried out to verify the binding site. HUVECs treated with miR-1264 mimic or control miRNA were co-transfected with psiCHECK2 constructs containing the WT or mutant circRNA-0006896 sites. (D) Confocal microscopy analysis of serum-Exos phagocytosis by HUVECs. (E) Colocalization of circRNA-0006896 with miR1264 in HUVECs Fluorescence in situ hybridization was used to evaluate the cellular localization of circRNA-0006896 and miR-1264. Nuclei were stained with DAPI. Relative expression levels of (F) circRNA-0006896, (G) miR-1264 and (H) DNMT1 in the mock and serum-Exos-treated HUVECs. Scale bar, 100 µm. Data are presented as the mean ± SD. ***P<0.001, **P<0.01, ### P<0.001 vs. SA-Exos. Serum-Exos, serum exosomes; circRNA, circular RNA; miRNA, microRNA; HUVEC, human umbilical vein endothelial cell; DNMT1, DNA methyltransferase 1; WT, wild-type; Mut, mutant; SA, stable plaque atherosclerosis; UA, unstable/vulnerable plaque atherosclerosis.
Article Snippet: The
Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Binding Assay, Luciferase, Reporter Gene Assay, Control, Transfection, Construct, Mutagenesis, Confocal Microscopy, Fluorescence, In Situ Hybridization, Staining
Journal: Molecular Medicine Reports
Article Title: circRNA-0006896-miR1264-DNMT1 axis plays an important role in carotid plaque destabilization by regulating the behavior of endothelial cells in atherosclerosis
doi: 10.3892/mmr.2021.11950
Figure Lengend Snippet: Highly expressed circRNA-0006896 encapsulated in UA-serum-Exos increases the proliferation and migration of HUVECs. (A) Protein levels of DNMT1, SOCS3, P-STAT3 and STAT3 were determined by western blot analysis. (B) MTT assay. (C) Wound healing assay. (D) Transwell assay. Scale bar, 100 µm. Data are presented as the mean ± SD. ***P<0.001, **P<0.01 vs. mock group; ### P<0.001, ## P<0.01 vs. SA-Exos. Exos, exosomes; HUVEC, human umbilical vein endothelial cell; DNMT1, DNA methyltransferase 1; SOCS3, suppressor of cytokine signaling 3; signal transducer and activator of transcription 3; P, phosphorylated; SA, stable plaque atherosclerosis; UA, unstable/vulnerable plaque atherosclerosis; OD, optical density.
Article Snippet: The
Techniques: Migration, Western Blot, MTT Assay, Wound Healing Assay, Transwell Assay