Journal: Genes and Immunity

Article Title: Transcriptome characterization of immune suppression from battlefield-like stress

doi: 10.1038/gene.2012.49

Figure Lengend Snippet: Comparison of transcript levels determined by two different assays and comparison of transcript levels with plasma protein levels. ( a ) Correlation of real-time QPCR and cDNA microarray data: real-time PCR reactions for each gene were carried out with three or more replicates. Trizol RNA isolation and cDNA microarrays were used. Significance levels: *0.001⩽ q <0.05, **1.0E-5⩽ q <0.001, *** q <1.0E-5— q stands for P -values after FDR correction. ( b ) Plasma protein concentrations (ELISA) compared with transcript levels (oligo and cDNA microarray): plasma concentrations of PRL, IGF1 and IGF2, TNFα and enzymatic activity of superoxide dismutase 1 (SOD1) assays were performed in triplicate on the plasma of 9 of the 10 soldiers who completed RASP. The IGF-I depletion is consistent with the finding of other investigators who measured its plasma concentration on similar subjects (*0.01⩽ P <0.05, **0.001⩽ P <0.01, *** P <0.001).

Article Snippet: Expression profiles of miRs were assayed using Agilent's human miRNA v3 microarrays (Agilent Technologies Inc.) consisting of 15k targets representing 961 miRs.

Techniques: Microarray, Real-time Polymerase Chain Reaction, Isolation, Enzyme-linked Immunosorbent Assay, Activity Assay, Concentration Assay

Journal: Genes and Immunity

Article Title: Transcriptome characterization of immune suppression from battlefield-like stress

doi: 10.1038/gene.2012.49

Figure Lengend Snippet: Expression of immune response genes in leukocytes exposed ex vivo to SEB. Leukocytes isolated from whole blood were treated with SEB (∼10 6 cells ml −1 in RPMI 1640 and 10% human AB serum at a final concentration of 100 ng ml −1 SEB). Total RNA was isolated using Trizol and expression levels were profiled using cDNA microarrays. Shown here are the 151 RASP-suppressed immune response genes that passed Welch's test and FDR correction ( q <0.05). ( a ) Lanes left to right: pre-RASP samples not exposed to SEB (control), pre-RASP samples exposed to SEB, post-RASP samples not treated with SEB, post-RASP samples exposed to SEB. For comparative visualization purpose, expression values of the other groups were transformed against the pre-RASP control samples (black lane). Heat map of the same data without transformation is given in the supplement . ( b ) Expression values in SEB exposed leukocytes (in both the pre- and post-RASP conditions) were compared with the corresponding SEB untreated groups (pre-RASP control and post-RASP stressed groups). ( c ) Heat map of the 151 immune response genes in SEB treated groups (in both pre- and post-RASP leukocytes) clustered after subtraction of the corresponding baseline responses (cluster after subtraction of their expressions in the corresponding untreated groups shown in Figure 4b). clearly shows poor response of post-RASP leukocytes towards SEB exposure compared with pre-RASP leukocytes.

Article Snippet: Expression profiles of miRs were assayed using Agilent's human miRNA v3 microarrays (Agilent Technologies Inc.) consisting of 15k targets representing 961 miRs.

Techniques: Expressing, Ex Vivo, Isolation, Concentration Assay, Transformation Assay

Journal: Molecular Cancer

Article Title: Reversal of chemosensitivity and induction of cell malignancy of a non-malignant prostate cancer cell line upon extracellular vesicle exposure

doi: 10.1186/1476-4598-12-118

Figure Lengend Snippet: EV-mediated transfer of proteins via Kinexus phospho-protein microarray analysis in DU145 cells co-cultured with PrEC EVs

Article Snippet: To determine the proteins that are involved in or might be responsible for “phenotypic switching”, we utilized Kinexus phospho-protein microarray analysis (Vancouver, BC) in the DU145 cells co-cultured with PrEC EVs.

Techniques: Microarray

Journal: BMC Genomics

Article Title: MicroRNA-34c is associated with emphysema severity and modulates SERPINE1 expression

doi: 10.1186/1471-2164-15-88

Figure Lengend Snippet: Demographics of the TPCH-KCO set (n = 29 patients) used in microRNA microarrays

Article Snippet: Cells of lung origin (BEAS-2B and HFL1) were profiled using mRNA microarrays (Illumina HumanHT-12 V3) after in vitro manipulation.

Techniques: