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microarray analyses mastercycler personal  (Eppendorf AG)
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Eppendorf AG microarray analyses mastercycler personal
The distribution of mitochondrial location analyses can be skewed. (A) Example of distribution of the Cy5/Cy3 fluorescence ratios (Rf) that can be obtained from a standard global gene expression experiment. Both repressed and activated genes are expected; the distribution is similar to a normal distribution. (B) Example of distribution of Rf that can be obtained from <t>microarray</t> experiments comparing total RNAs and mitochondria-associated RNAs. Only RNAs enriched in the Cy5 channel are expected: the distribution is skewed by enriched RNA.
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The distribution of mitochondrial location analyses can be skewed. (A) Example of distribution of the Cy5/Cy3 fluorescence ratios (Rf) that can be obtained from a standard global gene expression experiment. Both repressed and activated genes are expected; the distribution is similar to a normal distribution. (B) Example of distribution of Rf that can be obtained from microarray experiments comparing total RNAs and mitochondria-associated RNAs. Only RNAs enriched in the Cy5 channel are expected: the distribution is skewed by enriched RNA.

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Yeast Mitochondrial Transcriptomics

doi: 10.1007/978-1-59745-365-3_35

Figure Lengend Snippet: The distribution of mitochondrial location analyses can be skewed. (A) Example of distribution of the Cy5/Cy3 fluorescence ratios (Rf) that can be obtained from a standard global gene expression experiment. Both repressed and activated genes are expected; the distribution is similar to a normal distribution. (B) Example of distribution of Rf that can be obtained from microarray experiments comparing total RNAs and mitochondria-associated RNAs. Only RNAs enriched in the Cy5 channel are expected: the distribution is skewed by enriched RNA.

Article Snippet: 2.4 Labeled Complementary DNA Synthesis for Microarray Analyses Mastercycler personal (Eppendorf).

Techniques: Fluorescence, Gene Expression, Microarray