Journal: Research in Pharmaceutical Sciences

Article Title: Antidiarrheal activities of isovanillin, iso-acetovanillon and Pycnocycla spinosa Decne ex.Boiss extract in mice

doi:

Figure Lengend Snippet: Antidiarrheal activity of P. spinosa hydroalcoholic extract, isovanillin, iso-acetovanillon and propantheline on mice with MgSO 4 induced diarrhoea (0.5 ml, 10% solution) in comparison with that of corresponding vehicle treated controls. Incident of diarrhoea were assessed as number of wet defecation following MgSO4 administration. Data are expressed as mean ± SEM, n=10 for each group. *P

Article Snippet: In the current study, we have used castor oil and MgSO4 for inducing diarrhoea in mice to examine antidiarrheal effects of isovanallin and iso-acetovanillon in comparison with that of propantheline as a standard drug.

Techniques: Activity Assay, Mouse Assay

Journal: PLoS ONE

Article Title: Insights into the Molecular Basis of L-Form Formation and Survival in Escherichia coli

doi: 10.1371/journal.pone.0007316

Figure Lengend Snippet: Comparison of L-form E. coli and classical E. coli morphologies. A E. coli colony on L-form induction media (LIM) exhibiting typical “fried egg” morphology. B Classical E. coli colony on Brain Heart Infusion (BHI) agar. C E. coli colony on BHI+ 10% Sucrose and 0.125% MgSO4 (BHI+ sucrose control media). D E. coli penicillin G mutant colony on BHI+ Pen G. E Phase contrast of rod-shaped E. coli cells within a classical colony. Scale bar, 3 µm. F Phase contrast of coccoid cells within E. coli L-form colony agar squash. Scale bar, 3 µm. G Individual coccoid cells in soft agar LIM. Scale bar, 10 µm. H Transmission electron microscopy (TEM) photo of a coccoid cell within an L-form colony. Scale bar, 200 nm. All images are by Hoffman modulation and scale bars are 100 µm unless specified.

Article Snippet: Undiluted along with serial tenfold dilutions of cells were spread (100 µl) or spotted (10 µl) onto L-form induction media (LIM) which consisted of brain heart infusion broth (BHI) Becton Dickinson (BD) supplemented with 1% agar (BD), 10% sucrose, 0.125% MgSO4 , and 6000 µg (10,000 units )/ml of Penicillin G (Sigma).

Techniques: Mutagenesis, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy