Journal: Advanced Science

Article Title: VDLIN: A Deep Learning‐Based Platform for Methylcobalamin‐Inspired Immunomodulatory Compound Screening

doi: 10.1002/advs.202413775

Figure Lengend Snippet: Methylcobalamin (MCB) suppressed LPS‐induced inflammatory responses in RAW 264.7 cells. A) MCB (50 µmol/L) treatment suppressed Il1b expression induced by LPS (1 µg/mL) at multiple time points, including 0, 1, 3, 6, 9, 12, and 24 h, indicating a time‐dependent inhibitory effect. B) MCB reduced LPS (1 µg/mL)‐induced Il1b expression in a concentration‐dependent manner, with significant suppression observed at concentrations of 2, 5, 10, 20, and 50 µmol/L after 12 h of treatment, as determined by RT‐qPCR analysis. C‐D) MCB markedly downregulated the expression of pro‐inflammatory cytokines ( Il1b , Il6 , and Tnf ), two key inflammatory enzymes ( Nos2 and Ptgs2 ), and the type I interferon Ifnb1 following 12 h of LPS stimulation and treatment with 20 µmol/L MCB, as determined by RT‐qPCR analysis. E) Immunoblot analysis of Nos2 and Ptgs2 protein expression levels following 12‐h treatment with DMSO, LPS, LPS‐MCB, or MCB, with GAPDH as the internal loading control. F) MCB significantly reduced the mortality rate in mice (n = 10 per group) following LPS challenge (20 mg/kg). RT‐qPCR data were presented as means ± SEM from three independent experiments. Statistical significance was determined by one‐way ANOVA with Bonferroni's multiple comparisons test (B–D), paired‐samples t‐test (A), or the log‐rank test (F). * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: ABclonal supplied active recombinant mouse CSF‐2/GM‐CSF protein (catalog no. RP01206), while Lipopolysaccharides (LPS) (catalog no. HY‐D1056) and Methylcobalamin (catalog no. HY‐B0586) were sourced from MedChemExpress.

Techniques: Expressing, Concentration Assay, Quantitative RT-PCR, Western Blot, Control

Journal: Reproductive Medicine and Biology

Article Title: Effects of antioxidant co‐supplementation therapy on spermatogenesis dysfunction in relation to the basal oxidation–reduction potential levels in spermatozoa: A pilot study

doi: 10.1002/rmb2.12450

Figure Lengend Snippet: Changes in semen parameters and ORP/10 6 sperm in both treatment groups. Statistical assessments were performed not only on changes within the treatment group (Wilcoxon signed‐rank sum test), but also on post‐treatment results between the two groups (Wilcoxon rank sum test)

Article Snippet: Participants were allocated at a balanced one‐to‐one ratio to either the antioxidant co‐supplementation therapy group with 80 mg/day vitamin C, 40 mg/day vitamin E, 150 mg/day coenzyme Q10, and 220 mg/day flaxseed oil (two tablets of SO support II; Partners, Yokohama, Japan) or methylcobalamin therapy group with 1500 μg/day methylcobalamin (three tablets of Mecobalamin; Sawai Pharmaceutical, Osaka, Japan)., The participants underwent their assigned therapy for three months with monthly visits to outpatient clinics for compliance checks.

Techniques: Concentration Assay

Journal: Reproductive Medicine and Biology

Article Title: Effects of antioxidant co‐supplementation therapy on spermatogenesis dysfunction in relation to the basal oxidation–reduction potential levels in spermatozoa: A pilot study

doi: 10.1002/rmb2.12450

Figure Lengend Snippet: Changes in semen parameters via treatment in the low‐ORP group. Statistical assessments were performed not only on changes within the group (Wilcoxon signed‐rank sum test), but also on post‐treatment results between the two treatment groups (Wilcoxon rank sum test)

Article Snippet: Participants were allocated at a balanced one‐to‐one ratio to either the antioxidant co‐supplementation therapy group with 80 mg/day vitamin C, 40 mg/day vitamin E, 150 mg/day coenzyme Q10, and 220 mg/day flaxseed oil (two tablets of SO support II; Partners, Yokohama, Japan) or methylcobalamin therapy group with 1500 μg/day methylcobalamin (three tablets of Mecobalamin; Sawai Pharmaceutical, Osaka, Japan)., The participants underwent their assigned therapy for three months with monthly visits to outpatient clinics for compliance checks.

Techniques: Concentration Assay

Journal: Reproductive Medicine and Biology

Article Title: Effects of antioxidant co‐supplementation therapy on spermatogenesis dysfunction in relation to the basal oxidation–reduction potential levels in spermatozoa: A pilot study

doi: 10.1002/rmb2.12450

Figure Lengend Snippet: Changes in semen parameters via treatment in the high‐ORP group. Statistical assessments were performed not only on changes within the group (Wilcoxon signed‐rank sum test), but also on post‐treatment results between the two treatment groups (Wilcoxon rank sum test)

Article Snippet: Participants were allocated at a balanced one‐to‐one ratio to either the antioxidant co‐supplementation therapy group with 80 mg/day vitamin C, 40 mg/day vitamin E, 150 mg/day coenzyme Q10, and 220 mg/day flaxseed oil (two tablets of SO support II; Partners, Yokohama, Japan) or methylcobalamin therapy group with 1500 μg/day methylcobalamin (three tablets of Mecobalamin; Sawai Pharmaceutical, Osaka, Japan)., The participants underwent their assigned therapy for three months with monthly visits to outpatient clinics for compliance checks.

Techniques: Concentration Assay

Journal: Reproductive Medicine and Biology

Article Title: Effects of antioxidant co‐supplementation therapy on spermatogenesis dysfunction in relation to the basal oxidation–reduction potential levels in spermatozoa: A pilot study

doi: 10.1002/rmb2.12450

Figure Lengend Snippet: Area under the curve (AUC)‐receiver operating characteristic (ROC) of the pre‐treatment ORP group in predicting the improvement in sperm concentration by (A) the antioxidant co‐supplementation or (B) methylcobalamin treatments

Article Snippet: Participants were allocated at a balanced one‐to‐one ratio to either the antioxidant co‐supplementation therapy group with 80 mg/day vitamin C, 40 mg/day vitamin E, 150 mg/day coenzyme Q10, and 220 mg/day flaxseed oil (two tablets of SO support II; Partners, Yokohama, Japan) or methylcobalamin therapy group with 1500 μg/day methylcobalamin (three tablets of Mecobalamin; Sawai Pharmaceutical, Osaka, Japan)., The participants underwent their assigned therapy for three months with monthly visits to outpatient clinics for compliance checks.

Techniques: Concentration Assay

Journal: Reproductive Medicine and Biology

Article Title: Effects of antioxidant co‐supplementation therapy on spermatogenesis dysfunction in relation to the basal oxidation–reduction potential levels in spermatozoa: A pilot study

doi: 10.1002/rmb2.12450

Figure Lengend Snippet: Changes in ORP (mV/10 6 sperm/ml) via treatment in sperm concentration‐improved and not‐improved groups

Article Snippet: Participants were allocated at a balanced one‐to‐one ratio to either the antioxidant co‐supplementation therapy group with 80 mg/day vitamin C, 40 mg/day vitamin E, 150 mg/day coenzyme Q10, and 220 mg/day flaxseed oil (two tablets of SO support II; Partners, Yokohama, Japan) or methylcobalamin therapy group with 1500 μg/day methylcobalamin (three tablets of Mecobalamin; Sawai Pharmaceutical, Osaka, Japan)., The participants underwent their assigned therapy for three months with monthly visits to outpatient clinics for compliance checks.

Techniques: Concentration Assay