Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Simplified process flow diagram of the reference methane SCP production process, based on .

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Basis of calculation for the energy requirements of methane SCP production.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Energy requirements of methane SCP production per step in kWh over the dry mass of product.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques: Centrifugation

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Range of the share of global resources required to fulfill the minimum global human protein requirements, while accounting for 12% food waste.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Expected ramp-up speed of methane SCP production in terms of the global caloric human requirements fulfilled over time. The results shown reflect the use of the budget of similar industries, including regular and fast construction speeds.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Expected ramp-up speed of methane SCP production in terms of the global protein human requirements fulfilled over time, for different values of the protein content of the SCP product. The results shown reflect the use of the budget of similar industries, including regular- and fast-construction speeds.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Breakdown of the contributions to the wholesale production cost incurred per unit of methane SCP produced.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques: Produced

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Methane Single Cell Protein: Potential to Secure a Global Protein Supply Against Catastrophic Food Shocks

doi: 10.3389/fbioe.2022.906704

Figure Lengend Snippet: Retail cost of methane SCP for different cost scenarios in U.S. dollars per kilogram of dry SCP product.

Article Snippet: Key players currently pioneering the mass production of methane SCP are Calysta Inc., Unibio A/S, Circe Biotechnologie GmbH, and String Bio Pvt Ltd.

Techniques:

Journal: Chemical & Biomedical Imaging

Article Title: Recombinant–Chemosynthetic Biosensors for Probing Cell Surface Signaling of Red Blood Cells and Other Cells

doi: 10.1021/cbmi.4c00067

Figure Lengend Snippet: Figure 2. (A) Optimization of the cell labeling protocol in HeLa cells was performed under three different conditions: (left) cells are in MEM/ DFBS/PS containing HeLa media and purified sortase A and GCaMP6s-LPDTG proteins are in 1% PBS; (middle) cells and purified sortase A and GCaMP6s-LPDTG proteins are in 1% PBS; (right) cells are in 1% PBS and purified sortase A and GCaMP6s-LPDTG proteins are in elution buffer. (B) HeLa cells labeled with ExtraCal showed a reduction in ExtraCal fluorescence upon EDTA addition and its fluorescence intensity was increased upon addition of HBSS containing Ca2+ (n = 25 cells from 3 repeats). The plot shows fluorescence change of ExtraCal upon EDTA followed by HBSS-With- Ca2+ addition over the course of 6 min. The error bars represent SD (standard error of standard deviation). Scale bar = 5 μm. EDTA, ethylenediaminetetraacetic acid; HBSS, Hanks’ balanced salt solution.

Article Snippet: EDTA Disodium salt dihydrate (Fisher Scientific), HBSS-With-Ca2+ (Gibco), Ca2+ free HBSS (Gibco), ATP (Chem-Impex International, Inc.)), Rhod-2 AM (Cayman Chemical), YM-254890 (Cayman Chemical), oxyrase (Oxyrase, Inc.), MgCl2 hexahydrate (SigmaAldrich), PMSF (Research Products International), Lysozyme (Gold Biotechnology), Luciferin (Gold Biotechnology), and EZ-Run Prestained Rec Protein Ladder (Fisher Scientific).

Techniques: Labeling, Purification, Fluorescence, Standard Deviation